• Journal of Ginseng Research
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• 제24권2호
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• pp.74-78
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• 2000

인삼모상근의 생장과 ginsenosides의 함량을 높이기 위하여 생장조절제가 첨가되지 않은 1/2 MS 배지에 jasmonic acid와 methyl jasmonate의 농도와 처리시기를 달리하여 인삼모상근 KGHR울 세포주를 배양하였다. 인삼모상근 생장은jasmonic acid와 methyl jasmonate 모두 1$\mu\textrm{m}$ 농도에서 가장 양호하였으며 30$\mu\textrm{m}$ 이상 농도가 증가할수록 모상근생장이 감소하였다. 그러나 생장이 낮았던 jasmonic acid 10$\mu\textrm{m}$ 처리구와 methyl jasmonate 50$\mu\textrm{m}$에서 ginsenosides 함량과 생산성이 더 높았다. 배양시기별로 jasmonic acid와 methyl jasmonate의 처리 효과는 jasmonic acid는 배양 후4주, methyl jasmonate는 3주에 처리하는 것이 ginsenosides의 함량과 생산성을 높이는데 효과적이었다.

• 한국산림과학회지
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• 제99권3호
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• pp.331-336
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• 2010

생물반응기를 이용한 가시오갈피 부정근 배양시에 methyl jasmonate를 농도별 (0, 5, 10, 20, 40 및 80 ${\mu}M$)로 처리하여 부정근의 생장과 eleutheroside류 생산과의 관계를 조사하였다. Methyl jasmonate 처리농도별 부정근의 생장은 무처리구에서 5.4 g dry weight(DW)/L로 가장 높았으며 methyl jasmonate 농도가 증가할수록 부정근 생장은 감소하였다. 그러나 eleutheroside B의 함량은 methyl jasmonate 농도가 높을수록 증가하여 40 ${\mu}M$ 처리구에서 각각 359.9 ${\mu}g$/g DW로 생산량이 가장 많았다. 반면에 eleutheroside E와 $E_1$은 10 ${\mu}M$ 처리구에서 각각 798.1 ${\mu}g$/g DW, 19.7 ${\mu}g$/g DW로 생산량이 가장 많았다. 배지 1 L당 eleutheroside류의 총생산량은 10 ${\mu}M$ 처리구에서 3818.1 ${\mu}g$/L를 생산하여 가장 우수하였다. 20 ${\mu}M$의 methyl jasmonate 처리 후 15일간 eleutheroside류의 함량을 조사한 결과 eleutheroside B는 methyl jasmonate처리 후 12일째, eleutheroside E는 처리 후 3일째, eleutheroside $E_1$은 처리 후 9일째 각각 가장 많은 생산량을 보여 주었다.

• Molecules and Cells
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• 제27권1호
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• pp.75-81
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• 2009

The Arabidopsis gene AtLEC (At3g15356) gene encodes a putative 30-kDa protein with a legume lectin-like domain. Likely to classic legume lectin family of genes, AtLEC is expressed in rosette leaves, primary inflorescences, and roots, as observed in Northern blot analysis. The accumulation of AtLEC transcript is induced very rapidly, within 30 min, by chitin, a fungal wall-derived oligosaccharide elictor of the plant defense response. Transgenic Arabidopsis carrying an AtLEC promoter-driven ${\beta}$-glucuronidase (GUS) construct exhibited GUS activity in the leaf veins, secondary inflorescences, carpel heads, and silique receptacles, in which no expression could be seen in Northern blot analysis. This observation suggests that AtLEC expression is induced transiently and locally during developmental processes in the absence of an external signal such as chitin. In addition, mechanically wounded sites showed strong GUS activity, indicating that the AtLEC promoter responds to jasmonate. Indeed, methyl jasmonate and ethylene exposure induced AtLEC expression within 3-6 h. Thus, the gene appears to play a role in the jasmonate-/ethylene-responsive, in addition to the chitin-elicited, defense responses. However, chitin-induced AtLEC expression was also observed in jasmonate-insensitive (coi1) and ethylene-insensitive (etr1-1) Arabidopsis mutants. Thus, it appears that chitin promotes AtLEC expression via a jasmonate- and/or ethylene-independent pathway.

• Journal of Microbiology and Biotechnology
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• 제10권1호
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• pp.107-110
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• 2000

Elicited cells with methyl jasmonate continued to produce benzophenanthridine alkaloids throughout medium changes in suspension cultures of Eschscholtzia californica. Large increases in alkaloid production were observed by re-elitations with medium changes. The total alkaloid production increased during the successive elicitation steps reaching a maximum level on the 4th elicitation. The highest total alkaloid produced was 250 mg/I, which was 20fold higher than that of the single elicitation and 4-fold higher than that of the normal culture without elicitation. The large increases in alkaloid production in successive re-elicitations with medium changes are believed to be caused by the accumulation of the signal transduction compound, jasmonate.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권2호
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• pp.162-165
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• 2005

A series of fluorine and hydroxyl containing jasmonate derivatives, which were chemically synthesized in our institute, were investigated for their effects on the biosynthesis and heterogeneity of ginsenosides in suspension cultures of Panax notoginseng cells. Com-pared to the control (without addition of elicitors), $100{\mu}M$ of each of the jasmonate was added on day 4 to the suspension cultures of P. notoginseng cells. It was observed that, jasmonates greatly enhanced the ginsenoside content and the ratio of Rb group to Rg group (i.e. $(Rb_1\;+\;Rd)/(Rg_1\;+\;Re)$ in the P. notoginseng cells. Some of the synthetic jasmonates, such as pentafluoropropyl jasmonate (PFPJA), 2-hydroxyethyl jasmonate (HEJA) and 2-hydroxye-thoxyethyl jasmonate (HEEJA), could promote the ginsenoside content to $2.55\;\pm\;0.11,\;3.65\;\pm\;0.13\;and\;2.94\;\pm\;0.06$mg/100 mg DW, respectively, compared to that of $0.64\;\pm\;0.06$mg/100 mg DW for the control and $2.17\;\pm\;0.04$ mg/100 mg DW by the commercially available methyl jasmonate (MJA); and they could change the respective Rb:Rg ratio to $1.60\;\pm\;0.04,\;1.87\;\pm\;0.01\;and\;1.56\;\pm\;0.05$, compared to that of $0.47\;\pm\;0.01$ for the control and $1.42\;\pm\;0.06$ by MJA. The results suggest that suitable esterification of MJA with fluorine or hydroxyl group could in-crease the elicitation activity to induce plant secondary metabolism. The information obtained from this study is useful for hyper-production of heterogeneous products by plant cell cultures.

• 한국작물학회:학술대회논문집
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• 한국작물학회 1999년도 춘계 학술대회지
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• pp.116-117
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• 1999

• Journal of Plant Biotechnology
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• 제42권3호
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• pp.265-270
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• 2015

더덕은 예로부터 약용으로 사용 되어 왔으며, 더덕의 뿌리에는 약용으로 가치가 높은 여러 종류의 triterpenoid 사포닌이 포함되어 있다. 본 연구에서는 더덕의 모상근 생육과 methyl jasmonate (MeJA)처리에 의한 사포닌 합성의 효과를 연구하였다. 더덕 모상근에 MeJA를 처리한 결과 주사포닌인 lancemaside A, B, E의 축적은 MeJA 무처리 모상근 보다 약 15% 정도 감소하였다. 반면 마이너사포닌(foetidissimoside A와 aster saponin Hb)의 함량은 무처리 모상근 보다 약15% 정도로 증가하였다. 이 결과를 통해 MeJA처리가 triterpene 사포닌의 생산조절을 위해 사용될 수 있는 것으로 판단된다.

• Journal of Plant Biology
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• 제38권3호
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• pp.235-242
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• 1995

Effects of methyl jasmonate (MeJA) on ethylene production in tomato(Lycopersicon esculentum Mill.) hypocotyl segments and fruits were studied. Ethylene production in tomato hypocotyl segments was inhibited by the increasing concentratons of MeJA, and 450 $\mu$M of MeJA showed 50% inhibitory effect. Time course data indicate that this inhibitory effect of MeJA appeared after 3 h of incubation period and continued until 24 h. Inhibition of ethylene producton by MeJA was due to the decrease in 1-aminocyclopropane-1-carboxylic acid(ACC) synthase activity. However, MeJA treatment had no effect on ACC oxidase activity and the accumulaton of ACC oxidase mRNAs. MeJA also inhibited auxin-induced ethylene production by decreasing in ACC synthase activity. In contrast, MeJA stimulated ethylene production in tomato fruits. When 30 $\mu$L/mL MeJA was treated in a gaseous state, ethylene production doubled and this stimulating effect continued until 4 days. To investigate the mechanisms of MeJA on ethylene production, ACC synthase and ACC oxidase activities were examined after MeJA treatment. MeJA increased the activities of both ACC synthase and ACC oxidase, and induced ACC oxidase mRNA accumulation. These data suggest that MeJA plays distinct roles in the ethylene production in different tomato tissues. It is possible that MeJA affects differently the mechanisms of signal transuction leading to the ethylene biosynthesis.

• The Plant Pathology Journal
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• 제33권4호
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• pp.402-409
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• 2017

Cyclic dipeptides (CDPs) are one of the simplest compounds produced by living organisms. Plant-growth promoting rhizobacteria (PGPRs) also produce CDPs that can induce disease resistance. Bacillus vallismortis strain BS07 producing various CDPs has been evaluated as a potential biocontrol agent against multiple plant pathogens in chili pepper. However, plant signal pathway triggered by CDPs has not been fully elucidated yet. Here we introduce four CDPs, cyclo(Gly-L-Pro) previously identified from Aspergillus sp., and cyclo(L-Ala-L-Ile), cyclo(L-Ala-L-Leu), and cyclo(L-Leu-L-Pro) identified from B. vallismortis BS07, which induce disease resistance in Arabidopsis against Pseudomonas syringae infection. The CDPs do not directly inhibit fungal and oomycete growth in vitro. These CDPs require PHYTOALEXIN DEFICIENT4, SALICYLIC ACID INDUCTION DEFICIENT2, and NONEXPRESSOR OF PATHOGENESIS-RELATED PROTEINS1 important for salicylic acid-dependent defense to induce resistance. On the other hand, regulators involved in jasmonate-dependent event, such as ETHYLENE RECEPTOR1, JASMONATE RESPONSE1, and JASMONATE INSENSITIVE1, are necessary to the CDP-induced resistance. Furthermore, treatment of these CDPs primes Arabidopsis plants to rapidly express PATHOGENESIS-RELATED PROTEIN4 at early infection phase. Taken together, we propose that these CDPs from PGPR strains accelerate activation of jasmonate-related signaling pathway during infection.

• Animal cells and systems
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• 제7권4호
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• pp.337-343
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• 2003

Methyl jasmonate (MeJA) is a signal molecule in the activation of defense responses in plants. In this study, we isolated 15 MeJA-inducible genes by subtractive hybridization. These genes encode two myrosinase-binding proteins, five lipase-like proteins, a polygalacturonase inhibitor, a putative chlorophyll-associated protein, a terpene synthase, a dehydroascorbate reductase, an ascorbate oxidase, a cysteine protease, an O-methyltransferase, and an epithiospecifier protein. Northern analysis showed that most of the Chinese cabbage genes are barely expressed in healthy leaves, but are strongly induced by MeJA treatment. We also examined whether these MeJA-inducible genes were activated by ethethon, BTH, and Pseudomonas syringae pv. tomato (Pst), a nonhost pathogen of Chinese cabbage. The results showed that none of the MeJA-inducible genes was strongly induced by ethephon or by BTH. The genes encoding lipase-like proteins and a myrosinase-binding protein were weakly induced by Pst. Other MeJA-inducible genes were not activated at all by the pathogen.