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Isolation and Characterization of Methyl Jasmonate -Inducible Genes in Chinese Cabbage  

Park, Yong-Soon (Division of Life Sciences, College of Natural Sciences, Chungbuk National University)
Cho, Tae-Ju (Division of Life Sciences, College of Natural Sciences, Chungbuk National University)
Publication Information
Animal cells and systems / v.7, no.4, 2003 , pp. 337-343 More about this Journal
Abstract
Methyl jasmonate (MeJA) is a signal molecule in the activation of defense responses in plants. In this study, we isolated 15 MeJA-inducible genes by subtractive hybridization. These genes encode two myrosinase-binding proteins, five lipase-like proteins, a polygalacturonase inhibitor, a putative chlorophyll-associated protein, a terpene synthase, a dehydroascorbate reductase, an ascorbate oxidase, a cysteine protease, an O-methyltransferase, and an epithiospecifier protein. Northern analysis showed that most of the Chinese cabbage genes are barely expressed in healthy leaves, but are strongly induced by MeJA treatment. We also examined whether these MeJA-inducible genes were activated by ethethon, BTH, and Pseudomonas syringae pv. tomato (Pst), a nonhost pathogen of Chinese cabbage. The results showed that none of the MeJA-inducible genes was strongly induced by ethephon or by BTH. The genes encoding lipase-like proteins and a myrosinase-binding protein were weakly induced by Pst. Other MeJA-inducible genes were not activated at all by the pathogen.
Keywords
Brassica rapa; Chinese cabbage; Methyl jasmonate-inducible gene;
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1 Mackey D, Belkhadir Y, Alonso JM, Ecker JR, and Dangl JL (2003) Arabidopsis RIN4 is a target of the type III virulence effector AvrRpt2 and modulates RPS2-mediated resistance. Cell 112: 379-389   DOI   ScienceOn
2 McDowell JM and Dangl JL (2000) Signal transduction in the plant immune response. Trends Biochem Sci 25: 79-82   DOI   ScienceOn
3 Krueger J, Thomas CM, Golstein C, Dixon MS, Smoker M, Tang S, Mulder L, and Jones JDG (2002) A tomato cysteine protease required for Cf-2-dependent disease resistance and suppression of autonecrosis. Science 296: 744-747   DOI   ScienceOn
4 Lambrix V, Reichelt M, Mitchell-Olds T, Kliebenstein DJ, and Gershenzon J (2001) The Arabidopsis epithiospecifier protein promotes the hydrolysis of glucosinolates to nitriles and influences Trichoplusia ni herbivory. Plant Cell 13: 2793-2807   DOI
5 Axtell MJ and Staskawicz BJ (2003) Initiation of RPS2-specified disease resistance in Arabidopsis is coupled to the AvrRpt2-directed elimination of RIN4. Cell 112: 369-377   DOI   ScienceOn
6 Jakab G, Manrique A, Zimmerli L, Metraux J-P, and Mauch-Mani B (2003) Molecular characterization of a novel lipase-like pathogen-inducible gene family of Arabidopsis. Plant Physiol 132: 1-10   DOI
7 Lavid N, Wang J, Shalit M, Guterman I, Bar E, Beuerle T, Menda N, Shafir S, Zamir D, Adam Z, Vainstein A, Weiss D, Pichersky E, and Lewinsohn E (2002) O-methyltransferases involved in the biosynthesis of volatile phenolic derivatives in rose petals. Plant Physiol 129: 1899-1907   DOI   ScienceOn
8 Kim M, Lim C-J, and Kim D (2002) Transcription of Schizosaccharomyces pombe thioltransferase-1 in response to stress conditions. J Biochem Mol Biol 35: 409-413   DOI
9 Fodor J, Gullner G, Adam AL, Barna B, Komives T, and Kiraly Z (1997) Local and systemic responses of antioxidants to tobacco mosaic virus infection and to salicylic acid in tobacco: role in systemic acquired resistance. Plant Physiol 114: 1443-1451   DOI
10 Goerlach J, Volrath S, Knauf-Beiter G, Hengy G, Beckhove U, Kogel K-H, Oostendorp M, Staub T, Ward E, Kessmann H, and Ryals J (1996) Benzothiadiazole, a novel class of inducers of systemic acquired resistance, activates gene expression and disease resistance in wheat. Plant Cell 8: 629-643   DOI   ScienceOn
11 Pieterse CMJ and van Loon LC (1999) Salicylic acid-independent plant defence pathways. Trends Plant Sci 4: 52-58   DOI   ScienceOn
12 Yao C, Conway WS, Ren R, Smith D, Ross GS, and Sams CE (1999) Gene encoding polygalacturonase inhibitor in apple fruit is developmentally regulated and activated by wounding and fungal infection. Plant Mol Biol 39: 1231-1241   DOI   ScienceOn
13 Erksson S, Andreasson E, Ekbom B, Graner G, Pontoppidan B, Taipalensuu J, Zhang J, Rask L, and Meijer J (2002) Complex formation of myrosinase isoenzymes in oilseed rape seeds are dependent on the presence of myrosinase-binding proteins. Plant Physiol 129: 1592-1599   DOI   ScienceOn
14 Walz C, Juenger M, Schad M, and Kehr J (2002) Evidence for the presence and activity of a complete antioxidant defence system in mature sieve tube. Plant J 31: 189-197   DOI   ScienceOn
15 Whalen MC, Innes RW, Bent AF, and Staskawicz BJ (1991) Identification of Pseudomonas syringae pathogens of Arabidopsis and a bacterial locus determining avirulence on both Arabidopsis and soybean. Plant Cell 3: 49-59   DOI   ScienceOn
16 Taipalensuu J, Falk A, Ek B, and Rask L (1997) Myrosinase-binding proteins are derived from a large wound-inducible and repetitive transcript. Eur J Biochem 243: 605-611   DOI   ScienceOn
17 Takasaki T, Hatakeyama K, Suzuki G, Watanabe M, Isogai A, and Hinata K (2000) The S receptor kinase determines self-incompatibility in Brassica stigma. Nature 403: 913-916   DOI   ScienceOn
18 Delaney T, Uknes S, Vernooij B, Friedrich L, Weymann K, Negrotto D, Gaffney T, Gut-Rella M, Kessmann H, Ward E, and Ryals J (1994) A central role of salicylic acid in plant disease resistance. Science 266: 1247-1250   DOI   ScienceOn
19 Thompson JD, Higgins DO; and Gibson TJ (1994) CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673-4680   DOI
20 Sanmartin M, Drogoudi PA, Lyons T, Pateraki I, Barnes J, and Kanellis AA (2003) Over-expression of ascorbate oxidase in the apoplast of transgenic tobacco results in altered ascorbate and glutathione redox states and increased sensitivity to ozone. Planta 216: 918-928   DOI
21 Ryang S-H, Chung S-Y, Lee S-H, Cha J-S, Kim HY, and Cho T-J (2002) Isolation of pathogen-induced Chinese cabbage genes by subtractive hybridization employing selective adaptor ligation. Biochem Biophys Res Commun 299: 352-359   DOI   ScienceOn
22 De Vries S, Hoge H and Bisseling T (1988) Isolation of total and polysomal RNA from plant tissues. In: Gelvin SB and Schilperoot RA (ed), Plant Molecular Biology, Kluwer Academic Publishers, Dordrecht, pp B6/1-5
23 Dangl JL and Jones JDG (2001) Plant pathogens and integrated defence responses to infection. Nature 411: 826-833   DOI   ScienceOn
24 Brader G, Tas E, and Paiva ET (2001) Jasmonate-dependent induction of indole glucosinolates in Arabidopsis by culture filtrates of the nonspecific pathogen Erwinia carotovora. Plant Physiol 126: 849-860   DOI
25 Powell AL, van Kan J, ten Have A, Visser J, Greve LC, Bennett AB, and Labavitch JM (2000) Transgenic expression of pear PGIP in tomato limits fungal colonization. Mol Plant Microbe Interact 13: 942-950   DOI   ScienceOn
26 Ryals JA, Neuenschwander UH, Willits MG, Molina A, Steiner HY, and Hunt MD (1996) Systemic acquired resistance. Plant Cell 8: 1809-1819   DOI   ScienceOn
27 Min H-J, Park S-S, and Cho T-J (2001) A simple and efficient subtractive cloning method. J Biochem Mol Biol 34: 59-65
28 Nuernberger T and Scheel D (2001) Signal transmission in the plant immune response. Trends Plant Sci 6: 372-379   DOI   ScienceOn
29 Cheong J-J and Choi YD (2003) Methyl jasmonate as a vital substance in plants. Trends Genet 19: 409-413   DOI   ScienceOn
30 Brick DJ, Brumlik MJ, Buckley JT, Cao JX, Davies PC, Misra S, Tranbarger T J, and Upton C (1995) A new family of lipolytic plant enzymes with members in rice, Arabidopsis and maize. FEBS Lett 377: 475-480   DOI   ScienceOn
31 Benedict CR, Lu J-L, Pettigrew OW, Liu J, Stipanovic RD, and Williams HJ (2001) The cyclization of farnesyl diphosphate and nerolidyl diphosphate by a purified recombinant ${\delta}$-cadinene synthase. Plant Physiol 125: 1754-1765   DOI
32 Bent AF (1996) Plant disease resistance genes: function meets structure. Plant Cell 8: 1757-1771   DOI   ScienceOn
33 Li L, Popko JL, Zhang XH, Osakabe K, Tsai CJ, Joshi CP, and Chiang VL (1997) A novel multifunctional O-methyltransferase implicated in a dual methylation pathway associated with lignin biosynthesis in loblolly pine. Proc Natl Acad Sci USA 94: 5461-5466   DOI
34 Osbourn AE (1996) Preformed antimicrobial compounds and plant defense against fungal attack. Plant Cell 8: 1821-1831   DOI   ScienceOn
35 Park Y-S, Min H-J, Ryang S-H, Oh K-J, Cha J-S, Kim HY, and Cho T-J (2003) Characterization of salicylic acid-induced genes in Chinese cabbage. Plant Cell Rep 21: 1027-1034   DOI
36 Lee K-A and Cho T-J (2003) Characterization of a salicylic acid-and pathogen-induced lipase-like gene in Chinese cabbage. J Biochem Mol Biol 36: 433-441   DOI
37 Bashan Y, Sharon E, Okon Y, and Henis Y (1981) Scanning electron and light microscopy of infection and symptom development in tomato leaves infected with Pseudomonas tomato. Physiol Plant Pathol 19: 139-144   DOI