Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Effect of elicited by methyl jasmonate on the saponin contents of Codonopsis lanceolata

Elicitor처리가 더덕사포닌 함량에 미치는 영향

  • Kim, Ji-Ah (Division of Forest Biotechnology, Korea Forest Research Institute) ;
  • Bae, Kee-Hwa (Nakdonggang National Institute of Biological Resources) ;
  • Choi, Yong-Eui (Department of Forest Resources, Kangwon National University)
  • 김지아 (국립산림과학원 산림생명공학과) ;
  • 배기화 (국립낙동강 생물자원관) ;
  • 최용의 (국립강원대학교 산림자원학과)
  • Received : 2015.09.01
  • Accepted : 2015.09.22
  • Published : 2015.09.30
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Abstract

The roots of Codonopsis lanceolata (Campanulaceae) contain several kinds of triterpenoid saponin with high medicinal values, which have been used in traditional medicines. This study investigates the impacts of methyl jasmonate (MeJA) - adding time on the saponin synthesis and the hairy root growth of C. lanceolata. A significant decrease in major saponin (lancemaside of three kinds) content of hairy roots was observed with MeJA treatments. Contents of lancemaside A, B and E decreased about 15% more than non-treated hairy roots. In contrast, minor saponin (foetidissimoside A and aster saponin Hb) accumulation was about 15% higher than the non-treated hairy roots. These results suggest that MeJA treatment could be used in the production of teriterpene saponins.

더덕은 예로부터 약용으로 사용 되어 왔으며, 더덕의 뿌리에는 약용으로 가치가 높은 여러 종류의 triterpenoid 사포닌이 포함되어 있다. 본 연구에서는 더덕의 모상근 생육과 methyl jasmonate (MeJA)처리에 의한 사포닌 합성의 효과를 연구하였다. 더덕 모상근에 MeJA를 처리한 결과 주사포닌인 lancemaside A, B, E의 축적은 MeJA 무처리 모상근 보다 약 15% 정도 감소하였다. 반면 마이너사포닌(foetidissimoside A와 aster saponin Hb)의 함량은 무처리 모상근 보다 약15% 정도로 증가하였다. 이 결과를 통해 MeJA처리가 triterpene 사포닌의 생산조절을 위해 사용될 수 있는 것으로 판단된다.

Keywords

References

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