• 대한의생명과학회지
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• 제27권2호
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• pp.45-50
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• 2021

Coagulase-negative staphylococci (CoNS) are a typical group of microorganisms, and the recent advances in laboratory technology and medicine has dramatically modified their significance in medical practice. CoNS, which were previously classified as normal bacterial flora, have recently been reported to be associated with serious infectious diseases, such as surgical wound infection or periprosthetic joint infection. Representative CoNS include Staphylococcus epidermidis, S. haemolyticus, and S. saprophyticus, which are known to cause serious problems in biomaterial-based and prosthetic device infections, as well as to cause simple urinary tract infections in sexually active women. Over the last decade, the clinical isolation rate of CoNS has been increasing, and antibiotic resistance has also been occurring. This review aimed to investigate the incidence of CoNS infection and to use the results as basic data for the management of CoNS, with a focus on the isolation rate and antibiotic resistance in clinical surgery.

• 한국동물위생학회지
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• 제37권4호
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• pp.233-240
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• 2014

Salmonella spp. are one of the most common bacteria that causes heavy losses in swine industry and have implications for public health. In this study, the correlation between Salmonella seroprev-alence on farms and the isolation rate from slaughtered pigs was analyzed and the antimicrobial resistance of the isolated Salmonella spp. was investigated. A total of 3,001 serum samples for ELISA were collected from 17 farms during two consecutive years (2012-2013). The mean values of ELISA OD% for each 8 age groups were as follows; gilt 27.83 (n=472), sow 23.75 (n=367), 150 days (d) of pig 16.53 (n=278), 130 d 11.87 (n=366), 100 d 9.46 (n=378), 20 d 9.17 (n=394), 70 d 6.56 (n=382), 40 d 3.72 (n=364). From July 2013 to January 2014, a total of 53 (8.0%) Salmonella strains were isolated from 665 slaughtered pigs shipped from those 17 farms. The mean values of ELISA OD% for each age groups serum samples that were collected in the second half of 2013 showed a positive correlation at 100 d (0.61, P<0.05), 130 d (0.45, P<0.1) with the isolation rate of Salmonella spp. in the slaughtered pigs. All the isolates were identified by a real-time PCR and tested for antimicrobial susceptibility. As a result, the predominant serovar was S. Typhimurium (52.8%) and there were 15 strains showing their own antimicrobial resistance pattern. All strains were susceptible to amoxicillin, cefepime, ciprofloxacin and some of them were resistant to streptomycin and tetracycline (60%), ampicillin (53.3%), chloramphenicol (33.3%), respectively.

• 생약학회지
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• 제28권3호
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• pp.162-165
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• 1997

Repeated column chromatographic works of hexane fraction of Aster scaber afforded some volatile mixtures of two or three components, which process potent inhibitory activity(more than 90% of control at $50{\mu}g/ml$) to the resistance of multi-drug resistant Staphylococcus aureus SA2 when combined with chlorampenicol($50{\mu}g/ml$).

• 한국재료학회지
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• 제1권3호
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• pp.132-138
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• 1991

2충의 BPSG를 사용하는 서브마이크론 CMOS DRAM에 있어 전기적 특성에 관한 BPSG flow온도의 영향을 비교하였다. BPSG flow온도를 $850^{\circ}C/850^{\circ}C,\;850^{\circ}C/900^{\circ}C,\;900^{\circ}C/900^{\circ}C$의 3가지 다른 조합을 적용하여 문턱전압, 파괴전압, Isolation전압과 더불어 면저항과 접촉 저항을 조사하였다. $900^{\circ}C/900^{\circ}C$ flow의 경우 NMOS에서 문턱전압은 $0.8\mu\textrm{m}$ 미만의 채널길이에서 급격히 감소하나 PMOS 경우는 차이가 없었다. NMOS와 PMOS의 파괴전압은 각각 $0.7\mu\textrm{m}$와 $0.8\mu\textrm{m}$ 이하에서 급격히 감소하였다. 그러나 $850^{\circ}C/850^{\circ}C$ flow의 경우에는 NMOS와 PMOS모두 문턱전압과 파괴전압은 채널길이 $0.7\mu\textrm{m}$까지 감소하지 않았다. Isolation전압은 BPSG flow온도 감소에 따라 증가하였다. 면저항과 접촉 저항은 BPSG flow온도가 $900^{\circ}C$에서 $850^{\circ}C$로 감소됨에 따라 급격히 증가되었다. 이와 같은 결과는 열처리 온도에 따라 dopant의 확산과 활성화에 관련 있는 것으로 생각된다. 접촉 저항 증가에 대한 개선 방법에 대하여 고찰하였다.

• 한국토양비료학회지
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• 제45권4호
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• pp.593-601
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• 2012

Heavy metals and metalloids removal can be considered as one of the most important world challenges because of their toxicity and direct impact on human health. Many processes have been introduced but biological processes of remediation seem to offer the most suitable solution in terms of efficiency and low cost. Actinobacteria constitute one of the major microbial populations in soil, and this can be attributed to their adaptive morphological structure as well as their exceptional metabolic power. Among microbes, actinobacteria are morphologic intermediate between fungi and bacteria. Studies on microbial diversities in metal contaminated lands have shown that actinobacteria may constitute a dominantly active microbiota in addition to ${\alpha}$ Proteobacteria. Furthermore, isolation studies have shown metal removal mechanisms which are reminiscent of notable multiresistant strains, such as Cupriavidus metallidurans. Apart from members of genus Streptomyces, which produce more than 90% of commercialized antibiotics, and the nitrogen fixing Frankia, little attention has been given to other members of this phylum. This is because of difficult culture condition requirements and maintenance. In this review, we focused on specific isolation of actinobacteria and their potential applications in metal bioremediation and plant growth promotion.

• Journal of Microbiology
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• 제45권6호
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• pp.590-592
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• 2007

We report the isolation of Salmonella enterica serotype Typhimurium phage type DT104 (CCARM 8104) from swine in Korea. The CCARM 8104 isolate was resistant to nalidixic acid and showed reduced susceptibility to quinolones. The CCARM 8104 isolate had a missense mutation, Asp87Asn, in the quinolone resistance-determining region in gyrA and produced PSE-1. The CCARM 8104 isolate carried two different class 1 integrons, and the PSE-1 ${\beta}$-lactamase gene was inserted into a 1,200 bp class 1 integron. The presence of DT104 with pse-1 in an integron located in a plasmid and reduced susceptibility to quinolone in swine pose a significant threat of possible horizontal spread between swine and humans.

• 국제초고층학회논문집
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• 제3권3호
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• pp.173-183
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• 2014

Nakanoshima Festival Tower is a 200 m high-rise complex building which contains a renewed 2700-seat capacity concert hall known as "Festival Hall" and offices including headquarter of a news company. In order to build up an office tower on the hall which requires large open space, a giant truss system is employed. The giant trusses being composed of mega-trusses and belt-trusses support all the building weight above them and transfer the load to the outside of the hall. The building also requires high seismic resistance performance for a news company. Application of mid-story seismic isolation enables the building to satisfy high-level seismic resistance criteria.

• 한국마린엔지니어링학회:학술대회논문집
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• 한국마린엔지니어링학회 2001년도 추계학술대회 논문집(Proceeding of the KOSME 2001 Autumn Annual Meeting)
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• pp.57-61
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• 2001

In a main propulsion system of naval ship, DRME(Double Resilient Mounted Engine) is mostly adopted to ensure vibration isolation and shock resistance against underwater explosion weapon attack. In this paper, an analysis program for vibration and shock evaluation of DRME is presented. DRME is modelled as multi-rigid bodies with nonlinear mounts, and direct time integration method is introduced for shock analysis. The computed results are compared with those of foreign ones. Navy's proposed specifications are well satisfied with this program. This analysis program serves for the development of domestic DRME technology of naval ship.

• 약학회지
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• 제46권2호
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• pp.108-112
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• 2002

Drug resistance is one of the most significant impediments to successful chemotherapy of cancer. Multidrug-resistance (MDR) is characterized by decreased cellular sensitivity to anticancer agents due to the overexpression of P-glycoprotein. By employing a resistant subline of HCT15 to adriamycin (CL02), we undertook the screening for agents which were effective to multidrug-resistant cancer cells. As a result, a myxobacterial strain JW150 was selected for study since an activity against CL02 cells was discovered in the strain. Cytotoxicity-guided fractionation of the culture broth led to the isolation of cystothiazole A and melithiazole F. The producing organism was identified as Myxococcus stipitatus by taxonomic comparison with type strains of Myxococcus sp. as well as its morphological and physiological characteristics. Cystothiazole A and melithiazole F demonstrated potent cytotoxicity against certain human cancer cells with $IC_{50}$ values ranging from 0.03~ $0.72{\mu}{\textrm{g}}$/ml. Both compounds were interestingly as active against drug-resistant sublines CL02 and CP70 as against the corresponding parental cells.

• 대한수의학회지
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• 제56권1호
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• pp.37-40
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• 2016

A total of 131 Pasteurella (P.) multocida strains were isolated from the lungs of 1,064 pigs with respiratory clinical signs nationwide during 2009-2010 and 2013-2014. The strains of P. multocida comprised 77.1% serotype A and 22.9% serotype D. Analysis of a recent P. multocida outbreak in Korean pigs showed that the isolation rate of serotype D decreased annually. The incidence of antimicrobial resistance, as measured using minimal inhibitory concentration values, has decreased recently. Overall, further studies to characterize P. multocida isolated from pigs in Korea are needed to prevent P. multocida infection in the Korean swine industry