Korean Journal of Veterinary Service (한국동물위생학회지)

The Korean Society of Veterinary Service (한국동물위생학회)
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Correlation between the Salmonella seroprevalence on farms and the isolation rate from slaughtered pigs and antimicrobial resistance from the isolates

양돈장 살모넬라 혈청 양성율과 도축돈 살모넬라균 분리율의 상관관계 및 분리 균주의 항생제 내성

  • Received : 2014.07.31
  • Accepted : 2014.12.10
  • Published : 2014.12.30
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Abstract

Salmonella spp. are one of the most common bacteria that causes heavy losses in swine industry and have implications for public health. In this study, the correlation between Salmonella seroprev-alence on farms and the isolation rate from slaughtered pigs was analyzed and the antimicrobial resistance of the isolated Salmonella spp. was investigated. A total of 3,001 serum samples for ELISA were collected from 17 farms during two consecutive years (2012-2013). The mean values of ELISA OD% for each 8 age groups were as follows; gilt 27.83 (n=472), sow 23.75 (n=367), 150 days (d) of pig 16.53 (n=278), 130 d 11.87 (n=366), 100 d 9.46 (n=378), 20 d 9.17 (n=394), 70 d 6.56 (n=382), 40 d 3.72 (n=364). From July 2013 to January 2014, a total of 53 (8.0%) Salmonella strains were isolated from 665 slaughtered pigs shipped from those 17 farms. The mean values of ELISA OD% for each age groups serum samples that were collected in the second half of 2013 showed a positive correlation at 100 d (0.61, P<0.05), 130 d (0.45, P<0.1) with the isolation rate of Salmonella spp. in the slaughtered pigs. All the isolates were identified by a real-time PCR and tested for antimicrobial susceptibility. As a result, the predominant serovar was S. Typhimurium (52.8%) and there were 15 strains showing their own antimicrobial resistance pattern. All strains were susceptible to amoxicillin, cefepime, ciprofloxacin and some of them were resistant to streptomycin and tetracycline (60%), ampicillin (53.3%), chloramphenicol (33.3%), respectively.

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References

  1. 국립수의과학검역원. 2008. 동물질병 표준검사법. 국립수의과 학검역원 예규 제 65호.
  2. 농림축산식품부. 2013. 2012년도 축산 항생제내성균 감시체계 구축 보고서
  3. APHIS (Animal and Plant Health Inspection Service). 2009. Salmonella on U.S. swine sites-prevalence and antimicrobial susceptibility.
  4. Bahnson PB, Kim JY, Weigel RM, Miller GY, Troutt HF. 2005. Associations between on-farm and slaughter plant detection of Salmonella in market-weight pigs. J Food Prot 68: 246-250. https://doi.org/10.4315/0362-028X-68.2.246
  5. Choi WZ, Jung JH, Won HK, Kang ZW, Hahn TW. 2008. Serotypes and genotypes of Salmonella isolates from slaughtered pigs. Kor J Vet Serv 31: 1-16.
  6. CLSI. Clinical and Laboratory Standards Institute. 2014. Performance standards for antimicrobial susceptibility testing; Twenty-fourth informational supplement. M100-S24. Wayne, Pa, USA.
  7. EFSA (European Food Safety Authority). 2006. Risk assesment and mitigation options of Salmonella in pig production. EFSA Journal 4: 1-131.
  8. EFSA (European Food Safety Authority). 2008. Report of the Task Force on Zoonoses Data Collection on the analysis of the baseline survey on the prevalence of Salmonella in slaughter pigs, Part A, EFSA Journal 135: 1-111.
  9. Futagawa-Saito K, Hiratsuka S, Kamibeppu M, Hirosawa T, Oyabu K, Fukuyasu T. 2008. Salmonella in healthy pigs: Prevalence, serotype diversity and antimicrobial resistance observed during 1998-1999 and 2004-2005 in Japan. Epidemiol Infect 136: 1118-1123.
  10. Hurd HS, McKean JD, Griffith RW, Wesley IV, Rostagno MH. 2002. Salmonella enterica infections in market swine with and without transport and holding. Appl Environ Microbiol 68: 2376-2381. https://doi.org/10.1128/AEM.68.5.2376-2381.2002
  11. Hah DY, Ji DH, Jo SR, Park AR, Jung EH, Pakr DY, Lee KC, Yang JW, Kim JS, Kim HJ, Jung JH, Song IH, Kim AR, Lee JY, Kim YH. 2011. Prevalence of the antimicrobial resistance and resistance associated gene in Salmonella spp. isolated from pigs and cattle in slaughterhouse. Korean J Vet Serv 34: 45-54. https://doi.org/10.7853/kjvs.2011.34.1.045
  12. Jung HK, Lee SS, Kim CY, Sunwoo SY, Lyoo YS. 2011. Serovars distribution and antimicrobial resistance patterns of Salmonella spp. isolated from the swine farms and slaughter houses. Korean J Vet Res 51: 123-128.
  13. Kim JY, Bahnson PB, Troutt HF, Isaacson RE, Weigel RM and Miller GY. 1999. Salmonella prev-alence in market weight pigs before and after shipment to slaughter ISECSP: Production Epidemiology 139.
  14. Kim EM, Kim HK, Park SJ, Lee CS, Luo Y, Moon HJ, Yang JS, Park BK. 2007. Prevalence and antimicrobial resistance patterns of Salmonella spp. Isolated from different aged pigs in Korea. Korean J Vet Res 47: 395-398.
  15. Kim YH, Kwon IK, Han JH. 2010. Seroprevalence of swine salmonellosis in Korean swine herds. J Food Sci Ani Resour 30: 62-65. https://doi.org/10.5851/kosfa.2010.30.1.62
  16. Kim SR, Nam HM, Jang GC, Kim A, Kang MS, Chae MH, Jung SC, Kang D, Kim J, Korean Veterinary Antimicrobial Resistance Monitoring Group, Lim SK. 2011a. Antimicrobial resistance in Salmonella isolates from food animals and raw meats in Korea during 2010. Kor J Vet Publ Hlth 35: 246-254.
  17. Kim HB, Baek H, Lee SJ, Jang YH, Jung SC, Kim A, Choe NH. 2011b. Prevalence and antimicrobial resistance of Salmonella spp. and Escherichia coli isolated from pigs at slaughterhouses in Korea. African J Microbiol Res 5: 823-830. https://doi.org/10.5897/AJMR10.850
  18. Larsen ST, McKean JD, Hurd HS, Rostagno MH, Griffith RW, Wesley IV. 2003. Impact of commercial preharvest transportation and holding on the prevalence of Salmonella enterica in cull sows. J Food Prot 66: 1134-1138. https://doi.org/10.4315/0362-028X-66.7.1134
  19. Lee WW, Jung BY, Kim HT, Chung KT, Lee GR, Kim KH, Lee DS, Kim YH. 2003. Prevalence and antimicrobial susceptibility of Salmonella isolated from Korean slaughter pigs. Korean J Vet Serv 26: 313-321.
  20. MAFF (Ministry of Agriculture, Fisheries and Food) on behalf of the Agricultural Departments of England and Scotland. 2000. Code of practice for the prevention and control of Salmonella on pig farms.
  21. Nielsen B, Alban L, Stege H, Sorensen LL, Mogelmose V, Bagger J, Dahl J, Baggesen DL. 2001. A new Salmonella surveillance and control programme in Danish pig herds and slaughterhouses. Berl Munch Tierarztl Wochenschr 114: 323-326.
  22. Raul CM, Nemat A, Manuel C, and Kris R. 2008. Survey on Salmonella prevalence in slaughter pigs from Saskatchewan. Can Vet J. Aug 49: 793-796.
  23. Rostagno MH, Wesley IV, Trampel DW, Hurd HS. 2006. Salmonella prevalence in market-age turkeys on-farm and at slaughter. Poult Sci 85: 1838-1842. https://doi.org/10.1093/ps/85.10.1838
  24. Steinbach G, Kroell U. 1999. Salmonella infections in swine herds-epidemiology and importance for human diseases. Dtsch Tierarztl Wochenschr 106: 282-8.
  25. Steven AC, Alison EB, Ronald WG. 2012. Salmonellosis. pp. 821-833. In: Jeffrey J. Zimmerman, Locke A, Karriker, Alejandro Ramirez, Kent J. Schwartz, Gregory W. Stevenson(ed.). Disease of Swine. 10th ed. A John Wiley & Sons. Ames, Iowa, USA.