• Korean Journal of Microbiology
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• v.38 no.2
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• pp.81-85
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• 2002

A marine bacterium producing superoxide dismutase, strain number B446, was screened with nitrite quantitation method using hydroxy amine and photochemically generated superoxide ion, and the superoxide dismutase was purified through 35-75% ammonium sulfate precipitation, DEAE-Sephadex A-25 ion exchange chromatography, Sephadex G-200 gel filtration chromatography, and High-Q anion exchange chromatography to a yield of 6% and purification fold of 32.3.

• The Korean Journal of Food And Nutrition
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• v.7 no.1
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• pp.23-28
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• 1994

Soybean $\beta$-amylase was purified by DEAE-cellulose ion exchange chromatography, Sephadex G-100 gel chromatography, CM Sephadex C-50 ion exchange chromatography and CM Sephadex C-50 ion exchange rechromatography The purified enzyme showed 1, 020 unit/mg of specific activity. The purified enzyme was identified as homogenious by disc PAGE and analysis of reaction product.

• KSBB Journal
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• v.15 no.1
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• pp.76-79
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• 2000

Complex, ill-defined mixtures of natural origin are often used as nutrients in the production of biological products through microbial fermentation. Product yields are affected by variation in these natural products. Yeast extract is a typical example of these natural products. Since it is a mixture of amino acids, peptides and nucleic acids, its composition is not well characterized. In this study, we investigated the properties of thiamine hydrochloride, riboflavin and pyridoxine hydrochlride in yeast extract by using a gel filtration chromatography, ion exchange chromatography and high performance liquid chromatography. Yeast extract solution was fractionated by gel filtration chromatography and ion exchange chromatography, and then, each fraction was analyzed by using a high performance liquid chromatography.

• Bioindustry News
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• v.13 no.2
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• pp.13-17
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• 2000

새로운 분리공정으로써 크로마토그래피를 이용하고자 하는 노력들이 활발하게 진행되어지고 있는 가운데, 기존의 전통적인 크로마토그래피를 더욱 발전시킨 시도들이 행하여지고 있다. 여기에 부합하여 약 20여년 전부터 연구되기 시작한 Displacement ion-exchange chromatography는 현재 활발한 연구가 진행되어지고 있으며, 전형적인 preparative chromatography에 비해 많은 장점을 가지고 있다. Displcement ionexchange chromatography는 displacer를 사용하여, 고농도의 원료 물질을 효율적으로 단시간내에 처리할 수 있는 공정으로 기존의 preparative chromatography의 문제점이었던 꼬리끌기 현상과 희시현상을 대폭 감소시킬 수 있게 되었다. 따라서, displacement ion-exchange chromatography 공정에서의 가장 큰 과제인 비선형 등온선 영역에서의 거동과 분리공정 조작선의 결정 및 고성능의 displacer에 대한 연구가 요구되어지고 있으며, 본 총론에서는 이온교환 크로마토그래피 공정에서 displacement ion exchange chromatography를 이용한 단백질의 분리에 대하여 고찰하고자 한다.

• 한국생물공학회:학술대회논문집
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• 2005.04a
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• pp.343-347
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• 2005

The polysaccharides were extracted from fruiting body, mycelia, and cell-free broth of Agaricus blazei Murill. The crude polysaccharides were obtained by the ethanol addtion. They were further purified using ion-exchange chromatography and gel chromatography. Ion-exchange chromatography using DEAE-cellulose column separated neutral and acidic polysaccharides. Neutral polysaccharides were then purified with gel filtration chromatography. For single peak obtained from gel filtration chromatography was molecular weight was measured with Sepharose CL-6B. The same procedure with acidic polysaccharides were performed to get the purified polysaccharides.

• Journal of the Korean Chemical Society
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• v.11 no.2
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• pp.56-59
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• 1967

Mixtures of amines can be separated by elution chromatography on a chelating resin, Dowex A-1 loaded with nickel ions based on ligand exchange. Aqueous ammonia is used as the eluent. The method has proved particulary effective for separating aromatic amines.

• Korean Chemical Engineering Research
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• v.52 no.1
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• pp.98-105
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• 2014

Lysozyme amounts to 0.3% in egg white and functions as an agent of cell lysis and activator of tissue reconstruction. Ion exchange chromatography is the most useful method of separation among affinity chromatography, ion exchange chromatography, and ultra-filtration. The aim of present study is to find the optimum pH and temperature for the separation of lysozyme in egg white within cation exchange gel filled glass column. And we compared results of experiments with those of simulations. Phosphate buffer was used, and pH and temperature were varied as 5~7 and $25{\sim}40^{\circ}C$ respectively. RP-HPLC was the tool for the retention time identification and quantitative analysis of lysozyme. OriginPro 8 measured the peak area of lysozyme chromatogram and quantified the eluted lysozyme. Largest amount of lysozyme was separated under the conditions of pH 5 and T $25^{\circ}C$.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.39 no.5
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• pp.384-390
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• 2006

To improve the extractability of carnosine and the levels of pro-oxidants such as iron in eel (Anguilla japonica) extracts, we examined the effects of extraction time, temperature, ion exchange chromatography and ultrafiltration (UF). The respective protein and total iron were reduced approximately 55 and 60% at 60$^{\circ}C$, 63 and 70% at 80$^{\circ}C$, 68 and 76% at 100$^{\circ}C$ and 82 and 48% with ion exchange chromatography, respectively, compared to the untreated extract. However, there was no significant difference in the carnosine levels in the eel extracts. Ultrafiltration reduced the protein content of the extract by 52% compared with the untreated extract. UF reduced the protein contents of the samples from 60, 80, and 100% heat treatment and ion exchange chromatography treatment by 27, 50, 46 and 47%, respectively. UF reduced the total iron contents of the identical four treatments by 14, 22, 23, and 43%, respectively, while UF increased the carnosine by 23, 17, 20, and 6%, respectively.

• Korean Journal of Microbiology
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• v.38 no.4
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• pp.254-259
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• 2002

A psychrotrophic bacterium was isolated from Antarctic marine sediment and identified as Shewanella sp. species based on the biochemical properties and 16S rRNA sequence, and designated as Shewanella sp. L93. Extracellular protease produced by this strain was purified through ammonium sulfate precipitation, High-Q column chromatography, first gel permeation chromatography, BioScale Q2 ion exchange chromatography and second gel permeation chromatography, and basic properties of this enzyme were investigated.

• Applied Biological Chemistry
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• v.30 no.1
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• pp.88-94
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• 1987

To investigate ginseng oligopeptides with biological activities, the water extract was purified by ultra-filtration, gel filtration, ion-exchange and thin layer chromatography. Ultra-filtered water extract exhibited antilipolytic activity, inhibiting epinephrine-induced lipolysis in the isolated fat cells of rat epididymal adipose tissue. The filtrate was separated into 3 fractions by Sephadex G-25 gel filtration. Peptides were found only in the first fraction(S-FI). Saponine and sugars were also detected in tie fraction. S-FI fraction resolved further into 6 fractions by Dowex 50 ion-exchange chromatography. The sugar and saponine depleted fraction(P-F2) from the second chromatography showed antilipolytic activity. The P-F2 fraction revealed 6 spots on TLC. The 6 spots were isolated by TLC and identified as peptides.