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http://dx.doi.org/10.9713/kcer.2014.52.1.98

The Effect of pH and Temperature on Lysozyme Separation in Ion-exchange Chromatography  

Ko, Kwan-Young (Department of Chemical Engineering, Chungnam National University)
Kim, In-Ho (Department of Chemical Engineering, Chungnam National University)
Publication Information
Korean Chemical Engineering Research / v.52, no.1, 2014 , pp. 98-105 More about this Journal
Abstract
Lysozyme amounts to 0.3% in egg white and functions as an agent of cell lysis and activator of tissue reconstruction. Ion exchange chromatography is the most useful method of separation among affinity chromatography, ion exchange chromatography, and ultra-filtration. The aim of present study is to find the optimum pH and temperature for the separation of lysozyme in egg white within cation exchange gel filled glass column. And we compared results of experiments with those of simulations. Phosphate buffer was used, and pH and temperature were varied as 5~7 and $25{\sim}40^{\circ}C$ respectively. RP-HPLC was the tool for the retention time identification and quantitative analysis of lysozyme. OriginPro 8 measured the peak area of lysozyme chromatogram and quantified the eluted lysozyme. Largest amount of lysozyme was separated under the conditions of pH 5 and T $25^{\circ}C$.
Keywords
Lysozyme; Ion Exchange Chromatography; Aspen Simulation;
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Times Cited By KSCI : 3  (Citation Analysis)
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