Applied Biological Chemistry
- Volume 30 Issue 1
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- Pages.88-94
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- 1987
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- 2468-0834(pISSN)
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- 2468-0842(eISSN)
Extraction and Purification of Ginseng Oligopeptides with Antilipolytic Activities
Antilipolytic Activity를 보유하는 인삼 Oligopeptide의 추출 및 정제
- Kim, Su-Ill (Department of Agricultural Chemistry, College of Agriculture, Seoul National University) ;
- Na, Jee-Yeong (Department of Agricultural Chemistry, College of Agriculture, Seoul National University) ;
- Jo, Do-Hyun (A-Jou University) ;
- Lee, Chun-Yung (Kyung Hee University)
- Published : 1987.03.30
Abstract
To investigate ginseng oligopeptides with biological activities, the water extract was purified by ultra-filtration, gel filtration, ion-exchange and thin layer chromatography. Ultra-filtered water extract exhibited antilipolytic activity, inhibiting epinephrine-induced lipolysis in the isolated fat cells of rat epididymal adipose tissue. The filtrate was separated into 3 fractions by Sephadex G-25 gel filtration. Peptides were found only in the first fraction(S-FI). Saponine and sugars were also detected in tie fraction. S-FI fraction resolved further into 6 fractions by Dowex 50 ion-exchange chromatography. The sugar and saponine depleted fraction(P-F2) from the second chromatography showed antilipolytic activity. The P-F2 fraction revealed 6 spots on TLC. The 6 spots were isolated by TLC and identified as peptides.
인삼 성분중 생리적 활성을 가지고 있는 oligopeptide를 검출하고 분리하기 위하여 물 추출액을 ultra-filtration 한 후 gel filtration, ion-exchange chromatography 및 thin layer chromatography 등을 행하였다. 인삼 물 추출액으로부터 고분자 물질을 제거한 ultra-filtrate는 epinephrine에 의해 유도된 fat cell의 lipolysis를 저해하는 antilipolytic activity를 보유하고 있었으며 Sephadex G-25 gel filtration에 의해 3개 fraction으로 나뉘어졌다. 이중 첫번째 fraction(S-FI)만이 peptide 성분을 함유하고 있었으며 saponin 및 당도 검출되었다. S-FI fraction은
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