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Screening and Purification of Superoxide Dismutase Producing Marine Bacterium Using Photochemically Generated Superoxide Ion  

조기웅 (한국해양연구원 해양천연물연구실)
Publication Information
Korean Journal of Microbiology / v.38, no.2, 2002 , pp. 81-85 More about this Journal
Abstract
A marine bacterium producing superoxide dismutase, strain number B446, was screened with nitrite quantitation method using hydroxy amine and photochemically generated superoxide ion, and the superoxide dismutase was purified through 35-75% ammonium sulfate precipitation, DEAE-Sephadex A-25 ion exchange chromatography, Sephadex G-200 gel filtration chromatography, and High-Q anion exchange chromatography to a yield of 6% and purification fold of 32.3.
Keywords
marine bacteria; superoxide dismutase (SOD);
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