Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Screening and Purification of Superoxide Dismutase Producing Marine Bacterium Using Photochemically Generated Superoxide Ion

광화학적으로 제조된 Superoxide Radical을 이용한 Superoxide Dismutase를 생산하는 해양미생물의 탐색 및 효소정제

  • 조기웅 (한국해양연구원 해양천연물연구실)
  • Published : 2002.06.01
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Abstract

A marine bacterium producing superoxide dismutase, strain number B446, was screened with nitrite quantitation method using hydroxy amine and photochemically generated superoxide ion, and the superoxide dismutase was purified through 35-75% ammonium sulfate precipitation, DEAE-Sephadex A-25 ion exchange chromatography, Sephadex G-200 gel filtration chromatography, and High-Q anion exchange chromatography to a yield of 6% and purification fold of 32.3.

해양세균으로부터 superoxide dismutase를 생산하는 균주를 광화학적 superoxide ion 형성 및 nitrite정량방법으로 검색하여 고활성 균주 B-446을 확보하고 본 균주로부터 35-75% ammonium sulfate precipitation, DEAE-Sephadex A-25 ion exchange chromatography, Sephadex C-200 gel filtration chromatography, High-Q anion exchange chromatography그리고 HPLC-GPC를 이용하여 수율 6%, 정제도 32.3 배의 정제된 SOD를 얻었으며 정제 과정중 효소활성 분석에 본 광화학적 superoxide ion생성 방법으로 사용하였다.

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References

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