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Purification and Characterization of Extracellular Protease form Psychrotrophic Antarctic Bacteria  

조기웅 (한국해양연구원 해양천연물 연구실)
방지헌 (한국해양연구원 해양천연물 연구실)
홍혜원 (한국해양연구원 해양천연물 연구실)
박승일 (한국해양연구원 해양천연물 연구실)
이윤호 (한국해양연구원 해양천연물 연구실)
Publication Information
Korean Journal of Microbiology / v.38, no.4, 2002 , pp. 254-259 More about this Journal
Abstract
A psychrotrophic bacterium was isolated from Antarctic marine sediment and identified as Shewanella sp. species based on the biochemical properties and 16S rRNA sequence, and designated as Shewanella sp. L93. Extracellular protease produced by this strain was purified through ammonium sulfate precipitation, High-Q column chromatography, first gel permeation chromatography, BioScale Q2 ion exchange chromatography and second gel permeation chromatography, and basic properties of this enzyme were investigated.
Keywords
antarctica; extracellular protease; marine bacteria;
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