• Horticultural Science & Technology
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• v.28 no.4
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• pp.711-714
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• 2010

A new cultivar 'Daemang' ($Hibiscus$ hybrid 'Daemang') originated from the interspecific cross between $Hibiscus$ $sinosyriacus$ 'Seobong' and $Hibiscus$ $syriacus$ 'Namwon' to improve the flower quality in 2001. 'Daemang' was preliminarily selected as 'R-143' in 2003 for its stable flower quality with long red eye spot and named in 2006. The tree habit shows vigorous growth and is upright, so it can be used as a specimen tree or street tree. Characteristic tests such as leaf shape, leaf size, flower characteristics, flowering, and capsule size were conducted from 2004 to 2006. The characteristics succeed after grafting. 'Daemang' had pink color flower with red eye spot. The width of flower is 15.2 cm. Petal length and width are 8.0 cm and 6.4 cm, respectively. Leaves are 9.49 cm long and 8.72 cm wide. After the plant characteristics evaluation for 3 years (2004~2006), it was registered as a variety 'Daemang' in 2008.

• Korean Journal of Breeding Science
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• v.43 no.3
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• pp.180-183
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• 2011

'Yeomyeong', a new welsh onion (Allium fistulosum L.) variety, is developed by the Bioenergy Crop Research Center, NICS, RDA in 2004. This variety, as interspecific $F_1$ hybrid, is developed by a cross between onion (Allium. cepa L.) and welsh onion (A. fistulosum L). The first cross was conducted in 2003 between MOS8, onion male sterile line and G2, welsh onion inbred line. Horticultural and yield characteristics of this hybrid $F_1$ line was investigated in greenhouse for 2 years from 2004 to 2005 with fall cropping cultivation. It has a single pseudostem plant type, anthocyanin-colored pseudostem, and male sterile umbel. It showed intermediate plant type of the maternal parents in overall plant characteristics. 'Yeomyeong' is fall sawing variety and has higher plant height and pseudostem length than that of check variety 'Gumjang'. The yield potential of this variety was about 130.1MT/ha in greenhouse in spring harvseting season. This variety would be adaptable to the fall sowing cultivation in green house.

• The Journal of the Institute of Internet, Broadcasting and Communication
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• v.17 no.1
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• pp.1-11
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• 2017

Recently that require digital signatures handwritten for personal use customer information and agree to the Terms of Service agreement or a general sign up. Signature system including an existing handwritten signature are a problem, which may be a platform-dependent, as well as the environment in which to perform the signature vary according to device Status of presence because it is being utilized is implemented on the service platform itself within each company. In this paper, we designed and implemented an integrated system handwritten signature as possible using a cross-browser way to store the handwritten two-dimensional coordinates based on the jQuery it is interspecific directly integrated browser environment. iOS, Android, was tested in an integrated web browser in heterogeneous environments, including PC, it was confirmed that all handwritten signature function is working properly.

• Proceedings of the Korean Society of Crop Science Conference
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• 2017.06a
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• pp.23-23
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• 2017

Rice (Oryza sativa L.) is the world's most important cereal crop. In crop plant, chlorophyll content and leaf senescence could affect grain filling and yield. We analyzed a QTL associated with chlorophyll content and delayed leaf senescence using high chlorophyll near isogenic line (HC-NIL). HC-NIL derived from a cross between Oryza sativa cv. Hwaseong as a recurrent parent and wild species O. grandiglumis as a donor parent showed higher chlorophyll content than Hwaseong. To identify QTL associated with chlorophyll content, 58 $F_3$ and 38 $F_4$ lines were developed from a cross between HC-NIL and Hwaseong. For QTL analysis, simple sequence repeat (SSR) markers were used for genotyping and one-way ANOVA was conducted. A QTL for chlorophyll content (qCC2) was detected in chromosome 2 and explained 24.63% of phenotypic variation. The senescence effect of the qCC2 was examined in dark-induced incubation (DII). Detached leaves from Hwaseong and HC-NIL were incubated on 3mM MES buffer (pH 5.8) at $27^{\circ}C$ under complete dark condition. After 3 days of incubation, the Hwaseong leaves turned yellow, but the HC-NIL leaves were green. HC-NIL has higher chlorophyll content with delayed senescence than Hwaseong. These results indicated that qCC2 is associated with stay-green phenotype. To know whether the qCC2 is responsible for leaf functionality, ion leakage test and Fv/Fm measurement were performed. Both experiment results showed that differences were observed between Hwaseong and HC-NIL but it was not statistically significant. These results might suggest that the qCC2 is possibly related to chlorophyll content and non-functional stay-green phenotype.

• Korean Journal of Breeding Science
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• v.42 no.4
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• pp.357-364
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• 2010

In a previous study, we mapped 12 QTLs for 1,000 grain weight (TGW) in the 172 $BC_2F_2$ lines derived from a cross between Oryza sativa ssp. Japonica cv. Hwaseongbyeo and O. rufipogon. These QTLs explained 5.4 - 11.4% of the phenotypic variance for TGW. Marker-aided selection combined with backcrosses was employed to develop QTL-NILs for each QTL. $BC_2F_2$ lines with each target QTL were backcrossed to Hwaseongbyeo twice and then allowed to self to produce $BC_4F_5$ populations. SSR markers linked to TGW were employed to select QTL-NILs with the respective target QTL. Six QTL-NILs with the recurrent parent, Hwaseongbyeo were evaluated for nine traits for three years from 2007 and 2009. Differences were observed between each of the 6 QTL-NILs and Hwaseongbyeo in TGW. In addition to TGW, these QTL-NILs displayed differences in other agronomic traits possibly indicating a tight linkage of genes controlling these traits. The direction of the QTL for TGW in 6 QTL-NILs was consistent as in the $BC_2F_2$ lines from the same cross. Difference in TGW between each of the QTL-NILs and Hwaseongbyeo was associated with the difference in one or two grain shape traits; grain length, grain width, and grain thickness. SSR markers linked to the QTL for TGW will facilitate selection of the grain shape character in a breeding program to diversify grain shape and provide the foundation for map-based gene isolation. Also, the QTL-NILs developed in this report and the progenies from crosses between the QTL-NILs will be useful in clarifying epistatic interactions among QTLs for TGW.

• Korean Journal of Breeding Science
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• v.42 no.1
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• pp.100-108
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• 2010

This study was carried out to confirm the chromosome constitution and homoeologous recombination of progenies derived from various cross combination using tetraploid OA interspecific hybrid originated from mitotic chromosome doubling. Based on the chromosome analysis of progenies crossed reciprocally, there were only triploid progenies when crossed with diploid Asiatics as male or female parent. While only tetraploid progenies were produced when crossed tetraploid Asiatics or tetraploid OA hybrid with tetraploid OA hybrid, respectively. However, two types of progenies, that is, diploid and triploid plants, were produced from cross combinations between diploid Oriental hybrid and tetraploid OA hybrid. From the GISH analysis of OA hybrid, it was confirmed that diploid $F_1$ OA hybrid was consisted of 24 chromosomes (12 Oriental and 12 Asiatics) showing authentic OA hybrid. On the other hand, it was notified that triploid plants (3x=36) were consisted of 24 Asiatics lily chromosomes and 12 Oriental lily chromosomes by analysis of backcross progenies derived from either $A{\times}OA$ or $OA{\times}A$ crosses. In cross between tetraploid OA and OA, all the progenies were tetraploid with equal number of chromosomes without any homoeologous recombination, i.e. each 24 chromosomes of Oriental and Asiatics. In 2x-4x ($O{\times}OA$) cross combination, some progenies had 2x=24 chromosomes originated from only Oriental hybrid, and other progenies had 3x=36 chromosomes derived from 24 chromosomes of Oriental hybrid and 12 chromosomes of Asiatic hybrid. Only tetraploid Asiatics chromosomes without any Oriental one were produced in all the progenies from 4x-4x ($AA{\times}OA$) cross combination.

• Fisheries and Aquatic Sciences
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• v.5 no.2
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• pp.87-96
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• 2002

The physico-chemical properties of fresh milt of marbled sole, Limanda yokohamae, brown sole, Limanda herzensteini, starry flounder, Platichthys stellatus and olive flounder, Paralichthys olivaceus among flatfishes, and the fine structure of their cryopresserved spermatozoa were investigated. The highest concentration of sperm among these four species was $3.60\pm1.35\times10^{10}/mL$ in marbled sole. Osmolality and pH of seminal plasma in four flatfish species were approximately 330 mOsm/kg and 7.6-8.1, respectively. Seminal plasma compositions showed interspecific differences. The sperm heads of marbled sole, brown sole and starry flounder were ellipsoidal and that of olive flounder was round. The numbers of mitochondria of these four species were eight in marbled sole, seven in brown sole and starry flounder, and six in olive flounder. Cross-sectional view of flagellum showed typical 9+ 2 structure in all species. Most of sperms cryopreserved with a proper method had no visible ultrastructural changes after freeze-thawing, compared with the fresh sperm, but in a few cases, swelling of their heads and midpiece regions were observed.

• Korean Journal of Ichthyology
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• v.7 no.1
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• pp.71-78
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• 1995

Cobitis sinensis - longicorpus complex were commonly found in the upper stream of the Nakdong River in Korea and consisted of mostly with diploid and triploid karyotype forms. Among them, the triploid females of C. sinensis - longicorpus were artificially crossed with diploid males from C. sinensis and diploid males from C. longicorpus, respectively. The progenies from each cross produced the diploid individuals that possessed the paternal characters in their karyotypes and their body color patterns. Based on this results, we can assume that the triploid females, C. sinensis - longicorpus have a peculiar reproductive mode eliminating an uneven genome by the primary meiotic division and then producing a haploid ovum by secondary meiosis.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.52 no.3
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• pp.274-282
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• 2019

Female Siberian sturgeon Acipenser baerii were crossed with male Russian sturgeon A. gueldenstaedtii and the development, ontogenetic behavior, and phototactic characteristics of the resultant hybrid prolarvae were examined. The fertilization rate of the hybrid group was similar to that of Siberian sturgeon (the maternal species), and the overall developmental characteristics of hybrid embryos were not significantly different from those of either parental species. The time window from first hatch to the completion of hatching was wider in Russian sturgeon than in Siberian and hybrid sturgeon groups. The prolarval viability of the hybrid group was similar to that of the maternal species, and significantly higher than that of the paternal species. Hybrid prolarvae displayed ontogenetic behavioral patterns that were quite similar to those of Siberian sturgeon. In an illumination preference test, hybrid prolarvae displayed significant positive phototaxis, similar to the maternal species, and clearly distinct from the negative phototactic character of the paternal species. Taken together, our data indicate that the prolarval traits of hybrid sturgeon from this cross more closely resemble those of the maternal than the paternal species.

• Journal of The Korean Society of Grassland and Forage Science
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• v.9 no.2
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• pp.62-67
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• 1989

A breeding program in progress at Suweon Livestock Experiment Station, RDA, involves the hybridization of italian ryegrass (Lm) and several leading forage grasses in an effort to combine the nutritive, productivity and palatability qualities of Lm with the adaptive and cold resistance qual; .les of several leading grasses. In order to study the fate of the hybridization between remotely related species, immatured hybrid embryos were cultured on media. The emasculated plants of Lm were Sikem and Tetrone. Reweille, 2n=14, of perennial ryegrass (Lp.), Forager, 2n=42, of tall fescue(Fa), First, 2n=14,of meadow fescue(Fp), Potomac, 2n=28, of orchardgrass(Dg), and Richmond, 2n=42, of timothy(Ph.p) were used as pollinators. Embryos were isolated on 4, 8, 12, 16, and 20 days after pollination and cultured them on modified Murashige and Skoog media. Calluses and plantlets have been obtained after 8 days old embryos crossing between $Lm{\times}Fa$, after 12 days embryos crossing between $Lm{\times}Lp$, Dg, and Ph.p, and after 16 days embryos crossing between $Lm{\times}Fp$. Both callus and shoot formation occurred on 6 % or less of the plated embryos of $Lm{\times}Fp$, Dg, and Ph.p. Embryoderived callus forming shoots have been obtained from 4 days old embryo crossing between $Lm{\times}Dg$ which has not successfully been done anywhere as far as we know. It means that hybrid plants of species without crossability can be obtained through the use of immatured embryo culture. Some of plated embryos developed directly shoots from embryos and the others shoot-forming callus. Cross between related species showed a high frequency of directly shoot formation from plated embryos and cross between remotely related species a high frequency of callus formation.