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http://dx.doi.org/10.5657/fas.2002.5.2.087

Milt Properties of Four Flatfish Species and Fine Structure of Their Cryopreserved Spermatozoa  

Chang Yun Jeong (Department of Aquaculture, Pukyong National University)
Chang Young Jin (Department of Aquaculture, Pukyong National University)
Publication Information
Fisheries and Aquatic Sciences / v.5, no.2, 2002 , pp. 87-96 More about this Journal
Abstract
The physico-chemical properties of fresh milt of marbled sole, Limanda yokohamae, brown sole, Limanda herzensteini, starry flounder, Platichthys stellatus and olive flounder, Paralichthys olivaceus among flatfishes, and the fine structure of their cryopresserved spermatozoa were investigated. The highest concentration of sperm among these four species was $3.60\pm1.35\times10^{10}/mL$ in marbled sole. Osmolality and pH of seminal plasma in four flatfish species were approximately 330 mOsm/kg and 7.6-8.1, respectively. Seminal plasma compositions showed interspecific differences. The sperm heads of marbled sole, brown sole and starry flounder were ellipsoidal and that of olive flounder was round. The numbers of mitochondria of these four species were eight in marbled sole, seven in brown sole and starry flounder, and six in olive flounder. Cross-sectional view of flagellum showed typical 9+ 2 structure in all species. Most of sperms cryopreserved with a proper method had no visible ultrastructural changes after freeze-thawing, compared with the fresh sperm, but in a few cases, swelling of their heads and midpiece regions were observed.
Keywords
Flatfish; Marbled sole; Brown sole; Starry flounder; Olive flounder; Limanda yokohamae; Limanda herzensteini; Platichthys stellatus; Paralichthys olivaceus; Milt property;
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  • Reference
1 Stoss, J. and W. Holtz. 1981a. Cryopreservation of rainbowtrout (Salmo gairdnen) sperm. I. Effect of thawing solution, sperm density and interval between thawing and insemination. Aquaculture, 22, 97 - 104   DOI   ScienceOn
2 Stoss, J. and W. Holtz. 1981b. Cryopreservation of rainbowtrout (.Salmo gairdneri) sperm. II. Effect of pH and presence of a buffer in the diluent. Aquaculture, 25, 217-222   DOI   ScienceOn
3 Suquet, M., G. Dorange, M.H. Omnes, Y. Normant, A. LeRoux and C. Jauvel. 1993. Composition of the seminalfluid and ultrastructure of the spermatozoa of turbot(Scophthalmus maximus). J. Fish Biol., 42, 509-516   DOI   ScienceOn
4 Wang, Z. and L.W. Crim. 1997. Seasonal changes in thebiochemistry of seminal plasma and sperm motility inthe ocean pout, Macrozoarces americanus. Fish Physiol.Biochem., 16, 77-83   DOI   ScienceOn
5 Lin, J., L. Liu and K. Dabrowski. 1996. Characteristics of muskellunge spermatozoa I: Ultrastructure of spermatozoa and biochemical composition of semen. Trans. Am. Fish. Soc., 125, 187-194   DOI
6 Morisawa, M. 1985. Initiation mechanism of sperm motility at spawning in teleosts. Zool. Sci., 2, 605-615
7 Linhart, O., R. Billard and J.P. Proteau. 1993. Cryopreservation of European catfish (.Silurus glanis L.) spermatozoa. Aquaculture, 115, 347-359   DOI   ScienceOn
8 Mattei, X. 1991. Spermatozoon ultrastructure and its systematic implications in fishes. Can. J. Zool., 69, 3038-3055   DOI
9 Miyaki, K., K. Yoshikoshi and O. Tabeta. 1993. Scanning electron microscopic observation on cryopreserved spermatozoa in six species of puffer fishes. Nippon Suisan Gakkaishi, 59, 891p   DOI
10 Mohsawa, M., K. Suzuki and S. Morisawa. 1983a. Effects of potassium and osmolality on spermatozoa motility of salmonid fishes. J. Exp. Biol., 107, 10 5-113
11 Mohsawa, M., K. Suzuki, H. Shimizu, S. Morisawa and K. Yasuda. 1983b. Effects of osmolality and potassium on motility of spermatozoa from freshwater cyprinid fishes. J. Exp. Biol., 107, 95-103
12 Piironen, J. 1985. Variation in the properties of milt from thefinfish landlocked salmon (Salno salar m. sebago Girard) during a spawning season. Aquaculture, 48, 337-0   DOI   ScienceOn
13 Piironen, J. and H. Hyvarinen. 1983. Composition of the milt of some teleost fishes. J. Fish Biol., 22, 351-361   DOI
14 Stoss, J. 1983. Pish gamete preservation and spermatozoan physiology. Fish Physiol., 9B, 305-350
15 Gwo, J.C., H. Kurokura and R. Hirano. 1993. Cryopreservation of spermatozoa from rainbow trout, common carp, and marine puffer. Nippon Suisan Gakkaishi, 59, 777-782   DOI
16 Lahnsteiner, F. and R.A. Patzner. 1998. Sperm motility of the marine teleosts Boops boops, Diplodus sargus, Mullus barbatus and Trachurus mediterraneus. J. Fish Biol., 52, 726-742
17 Hwang, P.C. and D.R. Idler. 1969. A study of major cations,osmotic pressure and pH in seminal components of Atlantic salmon. J. Fish. Res. Bd. Can., 26, 413-419   DOI
18 Jamieson, B.G.M. 1991. Fish evolution and systematics: Evidence from spermatozoa, Cambridge University Press, New York, 319p
19 Kerby, J.H., J.D. Bayless and R.M. Harrell. 1985. Growth, survival, and harvest of striped bass produced with cryopreserved spermatozoa. Trans. Am. Fish. Soc., 114, 761-765   DOI
20 Lahnsteiner, F., B. Berger, T. Weismann and R. Patzner. 1996a. Changes in morphology, Physiology, metabolism and fertilization capacity of rainbow trout semen following cryopreservation. Prog. Fish-Cult., 58, 149-159   DOI   ScienceOn
21 Lahnsteiner, F., R.A. Patzner and T. Weismann. 1996b. Semen cryopreservation of salmonid fishes: Influence of handling parameters on the post-thaw fertilization rate. Aquaculture Res., 27, 659-671   DOI   ScienceOn
22 Lahnsteiner, F., T. Weismann and R.A. Patzner. 1996c. Cryopreservation of semen of the grayling (ThymaIIus thymallus) and the Danube salmon (.Hucho hucho). Aquaculture, 144, 265-274   DOI   ScienceOn
23 Lahnsteiner, F., T. Weismann and R.A. Patzner. 1992. Finestructural changes in spermatozoa of the grayling, Thy-mallus thymallus (Pisces: Teleostei) during routine cryo~preservation. Aquaculture, 103, 73-84   DOI   ScienceOn
24 Chao, N.H., W.C. Chao, K.C. Liu and I.C. Liao. 1987. Theproperties of tilapia sperm and its cryopreservation. J. Fish Biol., 30, 107-118   DOI
25 Chang, Y.J., H.K. Lim and K.H. Kho. 1995. Properties ofsemen and sperm motility in black seabream, Acanthopasrus schlegelj. J. Aquaculture, 8, 149-157 (in Korean)
26 Chang, Y.J., Y.J. Chang and H.K. Lim. 1998. Physico-chemical properties of milt and fine structure of cryopreserved :permatozoa in tiger puffer (Takifugu rubnpes).J. Korean Fish. Soc., 31, 353-358 (in Korean)
27 Chang, Y.J., H.K. Lim, Y.J. Chang, H.S. Kim and H.T. Huh.1999. Physico-chemical properties and cold storage of river puffer (Takifugu obscurus) milt. J. Korean Fish.Soc., 32, 243-246 (in Korean)
28 Ciereszko, A. and K. Dabrowski. 1993. Estimation of sperm concentration of rainbow trout, whitefish and yellowperch using a spectrophotometric technique. Aquaculture,109, 367-373   DOI   ScienceOn
29 Cruea, D.D. 1969. Some chemical and physical characteristicsof fish sperm. Trans. Am. Fish. Soc., 98, 785-788   DOI
30 Drokin, S., H. Stein and H. Brtscherer. 1998. Effect of cryopreservation on the fine structure of spermatozoa of rainbow trout (Oncorhynchus mykiss) and brown trout(Sahno trutta F. fario). Cryobiol., 37, 263-270   DOI   ScienceOn
31 Goodall, J.A., A.W. Blackshaw and M.F. Capra. 1989. Factors affecting the activation and duration of motility of the spermatozoa of the summer whiting (.Sillago ciliata). Aquaculture, 77, 243 - 250   DOI   ScienceOn
32 Bern, O. and R.R. Avtalion. 1990. Some morphological aspects of fertilization in tilapia. J. Fish Biol., 36, 375-381   DOI
33 Gwo, J.C. and C.R. Arnold. 1992. Cryopreservation of Atlantic croaker spermatozoa: Evaluation of morphological changes. J. Exp. Zool., 264, 444-453   DOI   ScienceOn
34 Baccetti, B., A.G. Burrini, G. Callaini, G. Gibertini, M. Mazzin. and S. Zerunian. 1984. Fish germinal cells. I. Comparative spermatology of seven cyprinids species. Gamete Res., 10, 373-396   DOI
35 Baynes, S.M. and A.P. Scott. 1987. Cryopreservation of rainbow trout spermatozoa: The influence of sperm quality, egg quality and extender composition on post-thaw fertility. Aquiaculture, 66, 53-67   DOI   ScienceOn
36 Billard, R. 1983. Ultrastructure of trout spermatozoa: Changesafter di ution and deep freezing. Cell Tissue Res., 228,205-218
37 Billard, R. and M.P. Cosson. 1992. Some problems related tothe assessment of sperm motility in freshwater fish. J. Exp. Zool., 261, 122-131   DOI   ScienceOn
38 Billard, R., A Cosson and L.W. Crim. 1993. Motility of freshand aged halibut sperm. Aquat. Living Resour., 6, 6775
39 Bouck, G.R. and J. Jacobson. 1976. Estimation of salmonid sperm concentration by microhematocrit technique.Trans. Am. Fish. Soc., 105, 534-535   DOI
40 Cabrita, E., R. Alvarez, L. Anel, K.J. Rana and M.P. Herraez.1998. Sublethal damage during cryopreservation of rainbow trout sperm. Cryobiol., 37, 245-253   DOI   ScienceOn