Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)
- Volume 9 Issue 2
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- Pages.62-67
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- 1989
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- 2287-5824(pISSN)
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- 2287-5832(eISSN)
Studies on the Interspecific and Intergeneric Hybridization in Herbage Grasses I. Effects of hybrid embryo age on callus formation and plant regeneration
화본과목초의 종.속간 잡종에 관한 연구 I. 교잡* 일령에 따른 Callus형성과 식물체재분화
Abstract
A breeding program in progress at Suweon Livestock Experiment Station, RDA, involves the hybridization of italian ryegrass (Lm) and several leading forage grasses in an effort to combine the nutritive, productivity and palatability qualities of Lm with the adaptive and cold resistance qual; .les of several leading grasses. In order to study the fate of the hybridization between remotely related species, immatured hybrid embryos were cultured on media. The emasculated plants of Lm were Sikem and Tetrone. Reweille, 2n=14, of perennial ryegrass (Lp.), Forager, 2n=42, of tall fescue(Fa), First, 2n=14,of meadow fescue(Fp), Potomac, 2n=28, of orchardgrass(Dg), and Richmond, 2n=42, of timothy(Ph.p) were used as pollinators. Embryos were isolated on 4, 8, 12, 16, and 20 days after pollination and cultured them on modified Murashige and Skoog media. Calluses and plantlets have been obtained after 8 days old embryos crossing between
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