• Title/Summary/Keyword: Intensity Approach

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Determination of Horseradish Peroxidase (HRP) using an Enhanced Chemiluminescence Assay (증강 화학발광 기법을 이용한 horseradish peroxidase(HRP)의 검량)

  • Kim, Wongee;Kim, Keunhan;Lee, Seungmok
    • Journal of Korean Society on Water Environment
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    • v.25 no.1
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    • pp.84-89
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    • 2009
  • Our nation's water resources remain susceptible to contamination by phenolic agrichemicals. These compounds can be toxic to a variety of organisms including humans. Their disposal is restricted in many countries with strict limits for acceptable concentrations in drinking water. Enzyme-mediated in situ stabilization has been advocated as an approach for the treatment of phenolic compounds in soils and groundwater. This study reports the development of a new approach to quantify the activity of the HRP enzyme in aqueous systems. The method is based on the coupled processes of energy transfer and enhanced chemiluminescence using a luminol-$H_2O_2$-HRP system. In this study, the effects of solution pH, ionic strength and aqueous concentrations of HRP, $H_2O_2$ and enhancer were evaluated on the p-iodophenol-enhanced, HRP-catalyzed chemiluminescence reaction intensity in Tris-HCl buffer. All assay components were found to affect the maximum chemiluminescene intensity. The calibration curve for HRP showed the linear relationship with maximum light intensity.

Analysis of Stress Intensity Factors for Interacting Two Growing Cracks (2개의 성장 균열들의 상호작용에 관한 응력확대계수 해석)

  • 박성완
    • Journal of the Korean Society of Manufacturing Technology Engineers
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    • v.9 no.5
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    • pp.47-57
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    • 2000
  • In this study, a fundamental approach to make clear the mechanism of the mutual interference and coalescence of stress fields in the vicinity of two crack tips on the process of their slow growth, using boundary element method. Automatic generation of quadratic discontinuous elements along both of the crack boundaries which can be defined by an arbitrary piece-wise straight geometry. The direction of the crack-extension increment is predicted by the maximum principal stress criterion, corrected to account for the discreteness of the crack extension. Along the computed direction, the crack is extended one increment. Automatic incremental crack-extension analysis with no remeshing, computation of the stress intensity factors by J-integral. Numerical stress intensity factors for two growing cracks in plane-homogeneous regions were determined.

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Computation of stress Intensity Factors of Hollow Cylinder with Three Dimension Inclination Cracks (3차원 경사크랙을 가진 중공축의 응력확대계수산정)

  • 이종선
    • Journal of the Korean Society of Manufacturing Technology Engineers
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    • v.8 no.1
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    • pp.21-27
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    • 1999
  • In this study, stress intensity factors KI, KII, KIII are existing at the same time to a hollow cylindrical bar of three dimension inclination crack. In order to investigate by experimentally the effect of the inclination angle $\psi$ of crack, artificial inclination cracks in the circumferential direction are put in the surface of a hollow cylindrical bar made by the epoxy-resin. Experimentally, stress analysis methods of stress intensity factors were proposed. But, suitable method are the caustic method and the photoelastic stress freezing method. The mixed mode of KI, and KII, were determined by the photoelastic method of the classical approach method and the FORTRAN language program of the used smallest square method.

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Adaptive Iterative Depeckling of SAR Imagery (반복 적응법에 의한 SAR 잡음 제거)

  • Lee, Sang-Hoon
    • Proceedings of the KSRS Conference
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    • 2007.03a
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    • pp.126-129
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    • 2007
  • In this paper, an iterative MAP approach using a Bayesian model based on the lognormal distribution for image intensity and a GRF for image texture is proposed for despeckling the SAR images that are corrupted by multiplicative speckle noise. When the image intensity is logarithmically transformed, the speckle noise is approximately Gaussian additive noise, and it tends to a normal probability much faster than the intensity distribution. The MRF is incorporated into digital image analysis by viewing pixel type s as states of molecules in a lattice-like physical system defined on a GRF. Because of the MRFGRF equivalence, the assignment of an energy function to the physical system determines its Gibbs measure, which is used to model molecular mteractions. The proposed adaptive iterative method was evaluated using simulation data generated by the Monte Carlo method. In the extensive experiments of this study, the proposed method demonstrated the capability to relax speckle noise and estimate noise-free intensity.

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Traffic Sign Recognition Considering the Intensity of Illumination (조도를 고려한 표지판 인식)

  • Cha, Yeon-Hwa;Chun, Chang-Mook;Kwon, Tae-Bum;Kang, Sung-Chul
    • The Journal of Korea Robotics Society
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    • v.6 no.2
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    • pp.173-181
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    • 2011
  • Recognition of traffic signs helps an unmanned ground vehicle to decide its behavior correctly, and it can reduce traffic accidents. However, low cost traffic sign recognition using a vision sensor is very difficult because the signs are exposed to various illumination conditions. This paper proposes a new approach to solve this problem using an illuminometer which detects the intensity of illumination. Using the intensity of illumination, the recognizer adjusts the parameters for image processing. Therefore, we can reduce the loss of information such as the shape and color of traffic signs. Experimental results show that the proposed method is able to improve the performance of traffic sign recognition in various weather and lighting conditions.

Text Region Extraction of Natural Scene Images using Gray-level Information and Split/Merge Method (명도 정보와 분할/합병 방법을 이용한 자연 영상에서의 텍스트 영역 추출)

  • Kim Ji-Soo;Kim Soo-Hyung;Choi Yeong-Woo
    • Journal of KIISE:Software and Applications
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    • v.32 no.6
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    • pp.502-511
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    • 2005
  • In this paper, we propose a hybrid analysis method(HAM) based on gray-intensity information from natural scene images. The HAM is composed of GIA(Gray-intensity Information Analysis) and SMA(Split/Merge Analysis). Our experimental results show that the proposed approach is superior to conventional methods both in simple and complex images.

A Projection-based Intensity Correction Method of Phased-Array Coil Images (위상 배열 코일 영상에서의 밝기 비균등성을 projection에 기반하여 수정하는 방법)

  • Yun SungDae;Chung Jun-Young;Han YeJi;Park HyunWook
    • Investigative Magnetic Resonance Imaging
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    • v.9 no.1
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    • pp.36-42
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    • 2005
  • Purpose : To develop a novel approach to calculate the sensitivity profiles of the phased array coil for use in non-uniform intensity correction. Materials and Methods : The proposed intensity correction method estimates the sensitivity profile of the coil to extract intensity variations that represent the scanned image. The sensitivity profile is estimated by fitting a non-linear curve to various angles of projections through the imaged object in order to eliminate the high-frequency image content. Filtered back projection is then used to compute the estimates of the sensitivity profile of each coil. The method was applied both to phantom and brain images from 8-channel phased-array coil and 4-channel phased-array coil, respectively. Results : Intensity-corrected images from the proposed method have more uniform intensity than those from the commonly used 'sum-of-squares' approach. By using the proposed correction method, the intensity variation was reduced to $6.1\%$ from $13.1\%$, acquired from the 'sum-of-squares'. Conclusion : The proposed method is more effective at correcting the intensity non-uniformity of the phased-array surface-coil images than the conventional 'sum-of-squares' method.

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A Method of Deriving an Intensity Mapping Function by Using The Variational Technique (변분법을 이용한 명암도 변환 함수 획득 방법)

  • Kim, Jun-Hyung;Noh, Chang-Kyun;Ko, Sung-Jea
    • Journal of the Institute of Electronics Engineers of Korea SP
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    • v.48 no.5
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    • pp.10-15
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    • 2011
  • Histogram equalization is an effective method to enhance the contrast of the image. However, it can result in unwanted artifacts such as excessive contrast enhancement and noise amplification. These artifacts can be reduced by modifying an intensity mapping function which is generated by histogram equalization. In this paper, we present a variational approach to the modification of the intensity mapping function. We define a functional whose minimization produces a modified intensity mapping function. Experimental results show that the intensity mapping function obtained by the proposed method can enhance the contrast of the image without visual artifacts.

T-stress solutions for cracks in rectangular plates with multiple holes

  • Yu, Jackie;Wang, Xin;Tan, Choon-Lai
    • Structural Engineering and Mechanics
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    • v.26 no.5
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    • pp.557-568
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    • 2007
  • The elastic T-stress is increasingly being recognized as an important second parameter to the stress intensity factor for fracture and fatigue assessments. In this paper, the mutual or M-contour integral approach is employed in conjunction with the Boundary Element Method (BEM) to determine the numerical T-stress solutions for cracks in plates with multiple holes. The problems investigated include plates of infinite width with multiple holes at which single or double, symmetric cracks have grown from. Comparisons of these results are also made with the corresponding solutions of finite plates with a single hole. For completeness, stress intensity factor solutions for the cracked geometries analyzed are presented as well. These results will be useful for failure assessments using the two-parameter linear elastic fracture mechanics approach.

Color Segmentation robust to Illumination Variations based on Statistical Methods of Hue and Saturation including Brightness (밝기 변화를 고려한 색상과 채도의 확률 모델에 기반한 조명변화에 간인한 컬러분할)

  • Kim, Chi-Ho;You, Bum-Jae;Kim, Hagbae
    • The Transactions of the Korean Institute of Electrical Engineers D
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    • v.54 no.10
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    • pp.604-614
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    • 2005
  • Color segmentation takes great attentions since a color is an effective and robust visual cue for characterizing one object from other objects. Color segmentation is, however, suffered from color variation induced from irregular illumination changes. This paper proposes a reliable color modeling approach in HSI (Hue-Saturation-Intensity) rotor space considering intensity information by adopting B-spline curve fitting to make a mathematical model for statistical characteristics of a color with respect to brightness. It is based on the fact that color distribution of a single-colored object is not invariant with respect to brightness variations even in HS (Hue-Saturation) plane. The proposed approach is applied for the segmentation of human skin areas successfully under various illumination conditions.