• Journal of Power Electronics
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• v.17 no.4
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• pp.905-913
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• 2017

To enhance the zero-voltage switching (ZVS) range and power density of the phase-shift full-bridge (PSFB) ZVS converters used in geophysical exploration, an additional resonant inductor is used as a leakage inductance and a blocking capacitor which is equivalent to interlayer capacitance is integrated into a novel integrated inductor-capacitor-transformer (L-C-T). The leakage inductance and equivalent interlayer capacitance of the novel integrated L-C-T are difficult to determine by conventional methods. To address this issue, this paper presents accurate and efficient methods to compute the leakage inductance and equivalent interlayer capacitance. Moreover, the accuracy of this methodology, which is based on electromagnetic energy and Lebedev's method, is verified by an experimental analysis and a finite element analysis (FEA). Taking the problems of the novel integrated L-C-T into consideration, the losses of the integrated L-C-T are analyzed and the temperature rise of the integrated L-C-T is determined by FEA. Finally, a PSFB ZVS converter prototype with the novel integrated L-C-T is designed and tested.

• Journal of Microbiology and Biotechnology
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• v.23 no.12
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• pp.1791-1801
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• 2013

Diketopiperazine is produced by various organisms, including bacteria, fungi, and animals, and has been suggested as a novel signal molecule involved in the modulation of genes with various biological functions. Vibrio vulnificus, which causes septicemia in humans, produces cyclo($\small{L}$-phenylalanine-$\small{L}$-proline) (cFP). To understand the biological roles of cFP, the effect of the compound on the expression of the total mRNA in V. vulnificus was assessed by next-generation sequencing. Based on the transcriptomic analysis, we classified the cFP-regulated genes into functional categories and clustered them according to the expression patterns resulted from treatment with cFP. From a total of 4,673 genes, excepting the genes encoding tRNA in V. vulnificus, 356 genes were up-regulated and 602 genes were down-regulated with an RPKM (reads per kilobase per million) value above 3. The genes most highly induced by cFP comprised those associated with the transport and metabolism of inorganic molecules, particularly iron. The genes negatively regulated by cFP included those associated with energy production and conversion, as well as carbohydrate metabolism. Noticeably, numerous genes related with biofilm formation were modulated by cFP. We demonstrated that cFP interferes significantly with the biofilm formation of V. vulnificus.

• Journal of Electrochemical Science and Technology
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• v.13 no.4
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• pp.431-437
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• 2022

As one of the most hazardous and deadliest pathogens, Listeria monocytogenes (LM) posed various serious diseases to the human being, thus designing effective strategy for its detection is of great significance. In this work, by preparing Ti₃C₂T_x MXenes nanoribbon (Ti₃C₂T_xR) as carrier and selecting thionine (Th) acted simultaneously as signal probe and functional monomer, a LM pathogen-imprinted polymers (PIP) integrated probe electrochemical sensor was design to monitor LM for the first time, that was carried out through the electropolymerization of Th on the Ti₃C₂T_xR/GCE surface in the existence of LM. Upon eluting the templates from the LM imprinted cavities, the fabricated PIP/Ti₃C₂T_xR/GCE sensor can rebound LM cells effectively. By recording the peak current of Th as the response signal, it can be weakened when LM cell was re-bound to the LM imprinted cavity on PIP/Ti₃C₂T_xR/GCE, and the absolute values of peak current change increase with the increasement of LM concentrations. After optimizing three key parameters, a considerable low analytical limit (2 CFU mL^-1) and wide linearity (10-10⁸ CFU mL^-1) for LM were achieved. In addition, the experiments demonstrated that the PIP/Ti₃C₂T_xR sensor offers satisfactory selectivity, reproducibility and stability.

• JSTS:Journal of Semiconductor Technology and Science
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• v.4 no.1
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• pp.41-44
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• 2004

The trend of decreasing the minimal structure sizes in microelectronics is still being continued. Therefore in its roadmap the Semiconductor Industries Association predicts a printed minimum MOS-transistor channel length of 10 nm for the year 2018. Although the resolution of optical lithography still dramatically increases, there are known and proved solutions for structure sizes significantly below 50 nm up to now. In this work a new method for the fabrication of extremely small MOS-transistors with a channel length and width below 50 nm with low demands to the used lithography will be explained. It's a further development of our deposition and etchback technique which was used in earlier research to produce transistors with very small channel lengths down to 30 nm, with a scaling of the transistor's width. The used technique is proved in a first charge of MOS-transistors with a channel area of W=200 nm and L=80 nm. The full CMOS compatible technique is easily transferable to almost any other technology line and results in an excellent homogeneity and reproducibility of the generated structure size. The electrical characteristics of such small transistor will be analyzed and the ultimate limits of the technique will be discussed.

• Journal of the Korean Mathematical Society
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• v.53 no.5
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• pp.1133-1148
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• 2016

In this paper, we first discuss a representation of solutions to the initial value problem and the initial-boundary value problem for discrete evolution equations $${\sum\limits^l_{n=0}}c_n{\partial}^n_tu(x,t)-{\rho}(x){\Delta}_{\omega}u(x,t)=H(x,t)$$, defined on networks, i.e. on weighted graphs. Secondly, we show that the weight of each link of networks can be uniquely identified by using their Dirichlet data and Neumann data on the boundary, under a monotonicity condition on their weights.

• Molecules and Cells
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• v.28 no.2
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• pp.111-117
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• 2009

The CHRM3 gene is a member of the muscarinic acetylcholine receptor family that plays important roles in the regulation of fundamental physiological functions. The evolutionary mechanism of exon-acquisition and alternative splicing of the CHRM3 gene in relation to transposable elements (TEs) were analyzed using experimental approaches and in silico analysis. Five different transcript variants (T1, T2, T3, T3-1, and T4) derived from three distinct promoter regions (T1: L1HS, T2, T4: original, T3, T3-1: THE1C) were identified. A placenta (T1) and testis (T3 and T3-1)-dominated expression pattern appeared to be controlled by different TEs (L1HS and THE1C) that were integrated into the common ancestor genome during primate evolution. Remarkably, the T1 transcript was formed by the integration event of the human specific L1HS element. Among the 12 different brain regions, the brain stem, olfactory region, and cerebellum showed decreased expression patterns. Evolutionary analysis of splicing sites and alternative splicing suggested that the exon-acquisition event was determined by a selection and conservation mechanism. Furthermore, continuous integration events of transposable elements could produce lineage specific alternative transcripts by providing novel promoters and splicing sites. Taken together, exon-acquisition and alternative splicing events of CHRM3 genes were shown to have occurred through the continuous integration of transposable elements following conservation.

• Proceedings of the KIPE Conference
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• 2003.07a
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• pp.36-39
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• 2003

The push pull forward converter is a very suitable circuit for low output voltage, high output current applications with a wide input voltage range. All the magnetic components (output inductor, transformer, input filter) can be integrated into a single core. The integrated magnetics can reduce the number of the magnetic components. Developed the push pull forward converter rating are of 36 $\~$72V input and 3.3V/30A output. In this converter, the efficiency was measured by $76.4\%$ at full load and 82.95$\%$ at full load. The maximum efficiency is up to 83.$\%$ at 200kHz switching frequency, l1A output.

• BMB Reports
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• v.49 no.2
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• pp.111-115
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• 2016

Caffeine has been proposed to have several beneficial effects on obesity and its related metabolic diseases; however, how caffeine affects adipocyte differentiation has not been elucidated. In this study, we demonstrated that caffeine suppressed 3T3-L1 adipocyte differentiation and inhibited the expression of CCAAT/enhancer binding protein (C/EBP)α and peroxisome proliferator-activated receptor (PPAR)γ, two main adipogenic transcription factors. Anti-adipogenic markers, such as preadipocyte secreted factor (Pref)-1 and Krüppel-like factor 2, remained to be expressed in the presence of caffeine. Furthermore, 3T3-L1 cells failed to undergo typical mitotic clonal expansion in the presence of caffeine. Investigation of hormonal signaling revealed that caffeine inhibited the activation of AKT and glycogen synthase kinase (GSK) 3 in a dose-dependent manner, but not extracellular signal-regulated kinase (ERK). Our data show that caffeine is an anti-adipogenic bioactive compound involved in the modulation of mitotic clonal expansion during adipocyte differentiation through the AKT/GSK3 pathway.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.56 no.3
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• pp.331-340
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• 2023

To estimate the biological reference points, suitable for fisheries management of sandfish Arctoscopus japonicas in the East Sea of Korea, we simulated the yield-per-recruit (Y/R) from age 0 to 6 (0-2,555 days). The stimulation was based on two instantaneous natural mortality conditions: size-dependent (M_t, d^-1) and constant (M_cons, d^-1); Subsequently, the biological reference points of the two mortality conditions was compared. M_t decreased from 0.0075 d^-1 to 0.0018 d^-1 depending on growth, and M_cons remained constant at 0.0011 d^-1 for all ages. Our Y/R model showed that the maximum yield of M_cons was 14 times higher than that of the M_t. The length at first capture to maximize the harvest at the F_0.1 points of the two natural mortality conditions was L_c,t=10.2 cm (TL) and L_c,cons=17 cm (TL). We concluded that M_t was more suitable for estimating M than M_cons; this is because L_c,t showed minimal difference from the current fishing regulations (11 cm, TL), and M_t reflected more biological characteristics than M_cons. We suggest that 10.2 cm and 0.8 as the suitable length at first capture and corresponding age, respectively for efficient fisheries management of sandfish.

• Journal of Korean Society on Water Environment
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• v.37 no.1
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• pp.47-54
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• 2021

Aerobic granular sludge (AGS) can be classified as a type of self-immobilized microbial aggregates measuring more than 0.2 mm. It offers the option to simultaneously remove COD, N, and P that occur in different zones inside a granule. Also, AGS is characterized by high precipitability, treatability with high organic loading, and high tolerance to low temperature. In this study, a sequencing batch reactor inoculated with AGS (AGS-SBR) is a new advanced wastewater treatment process that was proven to grow AGS with integrated nutrient removal and low C/N ratio. A pilot plant, AGS-SBR with a capacity of 225 ㎥/d was installed at an S sewage treatment plant in Gyeonggi-do. The results of the operation showed that the water quality of the effluent indicated that the value of BOD5 was 1.5 mg/L, CODMn was 11.4 mg/L, SS was 6.2 mg/L, T-N was 13.2 mg/L, and T-P was 0.197 mg/L, and all of these values reliably satisfied an effluent standard (I Area). In winter, the T-N treatment efficiency at a lower temperature of less than 11℃ also showed reliability to meet the effluent standard of the I Area (20 mg/L or less). Analysis of microbial community in AGS showed a higher preponderance of beneficial microorganisms involved in denitrification and phosphorus accumulation compared with activated sludge. The power consumption and sludge disposal cost were reduced by 34.7% and 54.9%, respectively, compared to the domestic SBR type sewage treatment plant with a processing capacity of 1,000 ㎥/d or less.