• Applied Biological Chemistry
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• v.19 no.3
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• pp.121-129
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• 1976

A high concentration of myo-Inositol in rat's milk was observed (61-91mg. of myo-Inositol per 100g of milk) by gas-liquid chromatographic method, using a 3% SE-52 column. Feeding experiments showed that approximately 85% of myo-Inositol in milk was from dietary origin: the rest was considered to be synthesized by 1L-myo-Inositol-1-phosphate lyase. Results suggested that the biosynthesis was not sufficiently high to permit the maintenance of its myo-Inositol level in milk. However, study $using(^{14}C)-glucose$ injection into lactating female rats confirmed biosynthesis of myo-Inositol from glucose in mammary gland. This biosynthesis reached a maximum within an hour after $(^{14}C)-glucose$ injection intraperitoneally as lactose biosynthesis did. Study using $(^3H)-myo-Inositol$ confirmed that most of the myo-Inositol in milk was transported from blood plasma myo-Inositol against a concentration gradient. About four hours after the beginning of the injection of $(^{14}C)-glucose$, the specific radioactivity of myo-Inositol in milk was 8% of that of glucose in the blood. When $(^3H)-myo-Inositol$ was injected, the specific radioactivity of myo-Inositol in milk was about 26% of that of blood six hours after injection.

• Journal of Life Science
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• v.19 no.10
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• pp.1456-1462
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• 2009

D-chiro-Inositol, which is the isomer of myo-inositol, is a well known drug for the treatment of type II diabetes. The methylated form of D-chiro-inositol, D-pinitol and D-chiro-inositol are synthesized when the plants are exposed to the abiotic stresses such as drought, salinity and low temperature as osmoprotectants. In soybean, myo-inositol is converted to ononitol by O-methyltransferase, and ononitol is converted to D-pinitol by ononitol epimerase and finally converted to D-chiro-inositol by demethylase. However there have been some reports that in buckwheat, myo-inositol can be converted to D-chiro-inositol directly. This study was conducted to determine the changes of soluble cyclitols in buckwheat seedlings after exposure to salt and drought stresses by GC-FID. The results indicated that myo-inositol may be the precursor of D-chiro-inositol biosynthesis.

• Applied Biological Chemistry
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• v.13 no.3
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• pp.197-205
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• 1970

Inositols are cyclohexanehexol and they have been known to be nine stereomers. Scyllo-inositol, epi-inositol and muco-inositol could be synthesized from myo-inositol. Scyllo-inositol and epi-inositol were obtained by oxidation and reduction process from myo-inositol. Myo-inositol and epi-inositol were oxidized by treatment, in solution, with dilute hydrogen peroxide. In all cases, only axial hydroxyl groups were oxidized and monoketons were obtained. Reduction of myo-inosose-2 with sodium boron hydride was carried out in $pH2{\sim}3.$ The reduction products were equatorial alcohol but: reduction of DL-epi-inosose-2 by catalytic reduction produced axial alcohol obtained. Inositol could be esterified. Hexa-O-(p-hydroxy benzoyl)-esters of myo-inositol, scyllo-inositol, epi-inositol and muco-inositol were synthesized and their antimicrobial action on microbes were tested for application to food industry. As the results, it was found that the activities of muco-inositol ester was more vigorous than others.

• Reproductive and Developmental Biology
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• v.28 no.2
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• pp.95-99
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• 2004

This study was carried out to assess whether the addition of myo-inositol to maturation medium could improve porcine oocyte maturation in vitro. Oocytes were cultured for the first 22 h in Witten's medium containing 10IU/$m\ell$ PMSG, 10 IU/$m\ell$ HCG supplemented with or without myo-inositol. Subsequently, they were cultured for additional 22 h in Witten's medium without hormone supplemented with or without myo-inositol. When the porcine oocytes were cultured in maturation medium containing myo-inositol, the proportion of metaphase II oocytes 44h after culture was higher in the myo-inositol group(P<0.05). To study effects of cumulus cell on the maturation induced by myo-inositol, we examined the maturation status of cumulus-enclosed or cumulus-denuded porcine follicular oocytes. The rates of maturation were significantly higher in the cumulus-enclosed oocytes(P<0.05). However, the maturation rates of cumulus-denuded oocytes cultured in medium containing myo-inositol were higher than those of control group(P<0.05). Our results suggest that myo-inositol may affect meiotic progression of porcine follicular oocytes and supplementation of myo-inositol in maturation medium may be useful for the in vitro maturation of porcine follicular oocytes.

• Journal of Life Science
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• v.15 no.2 s.69
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• pp.197-201
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• 2005

Studies with diabetic mammalian systems showed that chiro-inositol administration decreased blood glucose levels. We investigated which foodstuffs contain large amounts of chiro-inositol by surveying vegetables, edible plants and other staples in an effort to explore the nutritional or therapeutic supplements of chiro-inositol for diabetic patients. In the course of our investigation, we found that soybean and soybean derivatives have high chiro-inositol levels (upto 20 mg/g). The purified chiro-inositol from the soybean was then tested for reducing hyperglycemia by administrating the chiro-inositol in streptozotocin-treated diabetic rats. The results showed that the intragastric administration of 50 mg chiro-inositol/kg BW lowered hyperglycemia by $40\%$ and that the effect was sustained for approximately 12 hr.

• Korean Journal of Food Science and Technology
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• v.5 no.4
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• pp.249-257
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• 1973

In the previous work, as we have reported on the reduction of penta-O-acetyl-myo-inosose-2 and penta-O-acetyl-DL-epi-inosose-2, axial and equatorial alcohol have been produced. The synthesis of various inositol 5-nitro-2-furoate are described. The esters have been obtained by reaction of 5-nitro-2-furoylchloride with inositol stereomers in chloroform at low temperature. The ester have been characterized by paper chromatography and other determination. For the applicable purpose of food industry, their antimicrobial activities on microbes were tested, and it was found that the antimicrobial activity of muco-inositol ester was superior to the others, and considered that result would be related to the stereochemical structure.

• Proceedings of the Korean Society of Developmental Biology Conference
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• 2003.10a
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• pp.95-95
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• 2003

Inositol은 세포의증식 및 정보전달과정에 관여하는 phosphatidylinositol (PI)의 구성성분으로서 중요한 세포내 기능을 수행한다. P1는 세포내에서 특이적 인산화효소에 의하여 Pl4-phosphate(PIP), Pl4,5-phosphate($PIP_2$)로 변환되며 PIP$_2$는 phospholipase C(PLC)에 의하여 세포내 second messengers인 1,2-diacylglycerol (DAG)와 inositol 1,4,5-triphosphate ($IP_3$)로 변환된다. 이렇게 생산된 DAG와 IP3는 각각 protein kinase C의 활성과 $Ca^{2++}$의 동원에 관여하여 다양한 세포내 신호전달에 관여하는 것으로 보고되고 있다. 또한 mouse에서 $IP_3$의 작용에 의한 $Ca^{2++}$의 상승은 난모세포의 성숙분열이 촉진되고 돼지 난포세포에 있어서도 PI대사가 일어나고 있는 것으로 보고되고 있다. 본 연구에서는 돼지 미성숙 난모세포의 성숙과 단위발생에 미치는 inositol의 영향을 확인하기 위하여 실시하였다. inositol의 농도가 난포란의 성숙에 미치는 영향을 검토하기 위하여 난포란을 각각 $150 \mu mole$, $250 \mu mole$, $350 \mu mole$, inositol을 포함하는 Whitten's 배양액에서 44시간 성숙시킨 결과 $93.57 \pm 4.21, 93.91 \pm 2.71, 92.96 \pm 3.58%$가 성숙되어 대조구의 $87.10 \pm 4.21$보다 유의적(P<0.05)으로 차이를 나타내었다. 난포란의 등급에 따른 inositol의 영향을 확인하기 위하여 형태적으로 난구세포가 치밀한 난포란과 난구세포가 치밀하지 않은 난포란을 $250 \mu mole$ inositol을 포함하는 Whitten's 배양액에서 성숙을 유도한 결과 난구세포가 치밀한 난포란과 난구세포가 치밀하지 않은 난포란에서 inositol을 첨가하였을 때 성숙율은 각각 $95.35 \pm 2.22와 63.55 \pm 8.12$로 inositol을 첨가하지 않은 대조구보다($89.21 \pm 3.69와 48.56 \pm 8.99$) 유의적인 차이를 보였다. 난구세포가 inositol에 의한 성숙에 미치는 영향을 확인하기 위하여 난구세포를 제거한 난포란을 inositol을 포함하는 배지에서 성숙을 유도한 결과 inositol을 첨가하지 않은 처리구보다 양호한 성숙율을 보였다.

• Journal of environmental and Sanitary engineering
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• v.18 no.2
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• pp.60-66
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• 2003

This study was carried out to elucidate the preventive mechanism of molybdenum on lead-induced neuropathy, An animal model of lead neuropathy was induced by feeding diet containing lead to Sprague-Dawley rat for three weeks. Four weeks aged Sprague-Dawley rats were divided into four groups : normal control group, 10ppm-lead treated group, 1mg/kg-molybdenum treated group, 10ppm-lead and 1mg/kg-molybdenum treated group. The parameters on neuropathy were examined by measuring concentration of myo-inositol and myo-inosito uptake in sciatic nerve. In the lead-treated rats, myo-inositol concentration and myo-inositol uptake rate were reduced by from 54% to 33% respectively. This deficit results from that myo-inositol uptake system which is carrier mediated and sodium-potassium dependent was inhibited by the lead treatment. However, the molybdenum administration significantly eliminated the impairment and maintained myo-inositol concentration to about 82% of normal level. These results suggest that lead-induced neurotoxicity was significantly reduced by administration of molybdenum and the mechanism might be partly normalization of myo-inositol uptake system in sciatic nerve.

• Journal of environmental and Sanitary engineering
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• v.11 no.2
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• pp.21-26
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• 1996

In our previous studies, we reported that lead intoxicated nerve cell by inhibition of the Na$^{+}$-K$^{+}$ ATPase activity and reduction of myo-inositol in nerve cell. As the second series of experiments, in order to understand toxic mechanism of lead for nerve cell, the characteristics of myo-inositol transport system and the effect of lead on its system have been studied in the sciatic nerves of control and lead-treated rats. A lead intoxicated animal model was induced by feeding diet containing lead to Sprague-Dawley rat for two weeks. Four weeks aged Sprague-Dawley rats were divided into three group : normal control group, 10ppm-lead treated group, 100ppm-lead treated group. All rats were sacrified at the end of two weeks. The rate o myo-inositol transport by sciatic nerve isolated from lead-treated rat was significantly decreased compared with that of control rat. This deficit results from that myo-inositol transport system which is carrier mediated and sodium-potassium dependent was inhibited by the lead treatment (both 10ppm and 100ppm) due to increase of the Km value without affecting Vmax value for myo-inositol carrier. These observations suggest that the toxic mechanism of lead on nerve myo-inositol transport system might be a change of affinity without change of maximum transport velocity for carrier.

• Journal of Aquaculture
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• v.19 no.4
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• pp.225-230
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• 2006

A long-term (26 weeks) feeding experiment was conducted to examine the essentiality and requirement of inositol in diets for olive flounder because no information is available in the species. Five casein-gelatin based semi-purified diets were formulated to contain four different levels of myo-inositol (0, 0+antibiotic, 400, 800, and 1600 mg/kg, designated as M0, M0+, M400, M800, and M1600, respectively). One (M0+) of the control diets contained tetracycline hydrochloride (0.4%, wt/wt) as an antibiotic to inhibit biosynthesis of inositol by micro-organism in intestine of fish. Olive flounder at the early juvenile stage (initial body weight 1.22 g) were randomly distributed into fifteen 35 L tanks (48 fish/tank) and fed with one of the experimental diets (3 replicates per diet). At the end of the feeding trial, the weight gain, feed intake, specific growth rate, and protein efficiency ratio of fish fed diets containing higher levels of myo-inositol (M800 & M1600) were significantly higher than those of fish fed the other diets (P<0.05). Feed conversion ratio, survival, hematocrits, and hemoglobin of fish fed experimental diets were not significantly different among all the fish groups. Whole body compositions of fish were not different except for lipid content. The lipid content was significantly different between M0 and M400 diet groups. These results indicate that juvenile olive flounder requires dietary supplementation of myo-inositol in diets for normal growth and its optimum level seems to be approximately 800 mg myo-inositol/kg diet.