Journal of Aquaculture (한국양식학회지)

The Korean Society of Fisheries and Aquatic Science (한국양식학회)
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Myo-inositol Requirement in Diets for Juvenile Olive Flounder (Paralichthys olivaceus)

넙치 치어의 사료 내 myo-inositol 요구량

  • Lee, Bong-Joo (Faculty of Applied Marine Science, Cheju National University) ;
  • Lee, Kyeong-Jun (Faculty of Applied Marine Science, Cheju National University) ;
  • Pham, Minh-Anh (Faculty of Applied Marine Science, Cheju National University) ;
  • Lee, Sang-Min (Faculty of Marine Bioscience and Technology, Kangnung National University)
  • 이봉주 (제주대학교 해양과학대학) ;
  • 이경준 (제주대학교 해양과학대학) ;
  • 팜민안 (제주대학교 해양과학대학) ;
  • 이상민 (강릉대학교 해양생명공학부)
  • Published : 2006.11.25
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Abstract

A long-term (26 weeks) feeding experiment was conducted to examine the essentiality and requirement of inositol in diets for olive flounder because no information is available in the species. Five casein-gelatin based semi-purified diets were formulated to contain four different levels of myo-inositol (0, 0+antibiotic, 400, 800, and 1600 mg/kg, designated as M0, M0+, M400, M800, and M1600, respectively). One (M0+) of the control diets contained tetracycline hydrochloride (0.4%, wt/wt) as an antibiotic to inhibit biosynthesis of inositol by micro-organism in intestine of fish. Olive flounder at the early juvenile stage (initial body weight 1.22 g) were randomly distributed into fifteen 35 L tanks (48 fish/tank) and fed with one of the experimental diets (3 replicates per diet). At the end of the feeding trial, the weight gain, feed intake, specific growth rate, and protein efficiency ratio of fish fed diets containing higher levels of myo-inositol (M800 & M1600) were significantly higher than those of fish fed the other diets (P<0.05). Feed conversion ratio, survival, hematocrits, and hemoglobin of fish fed experimental diets were not significantly different among all the fish groups. Whole body compositions of fish were not different except for lipid content. The lipid content was significantly different between M0 and M400 diet groups. These results indicate that juvenile olive flounder requires dietary supplementation of myo-inositol in diets for normal growth and its optimum level seems to be approximately 800 mg myo-inositol/kg diet.

본 연구는 넙치 치어에 있어서 수용성 비타민인 myo-inositol의 사료 내 적정 요구량을 평가하고, 결핍 시 어류에 미치게 되는 생리적 영향을 조사하고자 수행 되었다. 실험에 사용된 어류는 평균무게가 1.22 g인 넙치 초기치어를 사용하였으며, 총 15개의 35 L 원형수조에 각 수조당 48 마리씩(3반복구) 무작위 배치하였다. 반 정제 사료원을 기초로한 총 5개의 실험사료는 52%의 조단백질과 18.3 MJ/kg diet의 에너지함량을 갖도록 조성되었고 myo-inositol 함량을 각각 0, 0, 400, 800, 1600 mg/kg diet으로 기초사료에 첨가하였다(M0, M0+, M400, M800, M1600). 실험사료 중 M0+ 사료는 항생제인 tetracycline hydrochloride 및 (SIGMA, USA)를 실험사료에 0.4%로 첨가함으로써, myo-inositol이 넙치의 장내 미생물들에 의하여 합성되는지를 알아보기 위해 설계되었다. 26주간의 사료공급 실험결과, 사료 내 myo-inositol 첨가에 의한 어류의 성장률 결과들에서 유의적인 차이가 나타났으며, myo-inositol 결핍사료를 섭취한 실험어류에서 성장지연과 같은 결핍증상을 보였다. Hematocrit과 Hemoglobin을 측정한 혈액분석과 간 지방함량 분석에서는 실험구 사이에서 차이가 나타나지 않았으나, 전어체 지방분석 결과에서는 단지 M0와 M400 사이에서 유의적인 차이를 나타내었다. 초기 넙치 치어의 myo-inositol 요구량은 성장률을 기초로 한 broken-line regression model (Robbins, 1986)에서 반 정제사료를 기초로 하였을 때 800 mg/kg로 결정되었다.

Keywords

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