Reproductive and Developmental Biology

The Korean Society of Animal Reproduction (한국동물번식학회)
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Effect of Myo-Inositol on In Vitro Maturation of Porcine Oocytes

Myo-inositol이 돼지 난모세포의 체외성숙에 미치는 영향

  • 조인식 (전남대학교 농업생명과학대학, 동물자원학부) ;
  • 한효원 (전남대학교 농업생명과학대학, 동물자원학) ;
  • 이상미 (전남대학교 농업생명과학대학, 동물자원학) ;
  • 박효영 (전남대학교 농업생명과학대학, 동물자원학) ;
  • 정영희 (전남대학교 농업생명과학대학, 동물자원학) ;
  • 문승주 (전남대학교 농업생명과학대학, 동물자원학) ;
  • 강승률 (농촌진흥청 난지농업연구) ;
  • 강만종 (전남대학교 농업생명과학대학, 동물자원학부)
  • Published : 2004.06.01
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Abstract

This study was carried out to assess whether the addition of myo-inositol to maturation medium could improve porcine oocyte maturation in vitro. Oocytes were cultured for the first 22 h in Witten's medium containing 10IU/$m\ell$ PMSG, 10 IU/$m\ell$ HCG supplemented with or without myo-inositol. Subsequently, they were cultured for additional 22 h in Witten's medium without hormone supplemented with or without myo-inositol. When the porcine oocytes were cultured in maturation medium containing myo-inositol, the proportion of metaphase II oocytes 44h after culture was higher in the myo-inositol group(P<0.05). To study effects of cumulus cell on the maturation induced by myo-inositol, we examined the maturation status of cumulus-enclosed or cumulus-denuded porcine follicular oocytes. The rates of maturation were significantly higher in the cumulus-enclosed oocytes(P<0.05). However, the maturation rates of cumulus-denuded oocytes cultured in medium containing myo-inositol were higher than those of control group(P<0.05). Our results suggest that myo-inositol may affect meiotic progression of porcine follicular oocytes and supplementation of myo-inositol in maturation medium may be useful for the in vitro maturation of porcine follicular oocytes.

본 연구는 미성숙돼지 난모세포의 체외 성숙에 있어서 myo-inositol의 영향을 알아보기 위하여 실시하였다. 미성숙 돼지 난모세포을 myo-inositol을 포함하는 또는 포함하지 않은 체외 성숙배지에서 44시간 체외 성숙을 유도하였을 때 성숙율은 myo-inositol을 첨가한 성숙배지에서 성숙시킨 실험구에 유의하게 높았다(P<0.05). Myo-inositol 에 의한 성숙율 향상에 있어서 난구세포의 영향을 알아보기 위하여 난구세포의 치밀도에 따라 분류하여 미성숙 난모세포을 myo-inositol을 포함하는 체외 성숙배지에서 배양하였을 때 난구세포가 치밀한 미성숙 난모세포에서가 더 높은 성숙율을 나타내었다(P<0.05). 그러나 난구세포가 치밀하지 않은 미성숙 난모세포도 myo-inositol을 포함하는 성숙배지에서 배양하면 성숙율은 대조구에 비하여 유의하게 높았다(P<0.05). 이러한 결과는 돼지 난모세포의 체외 성숙배지에 myo-inositol의 첨가는 체외 성숙율을 향상시킬 수 있음을 나타내고 있다.

Keywords

References

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