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Effect of Myo-Inositol on In Vitro Maturation of Porcine Oocytes  

조인식 (전남대학교 농업생명과학대학, 동물자원학부)
한효원 (전남대학교 농업생명과학대학, 동물자원학)
이상미 (전남대학교 농업생명과학대학, 동물자원학)
박효영 (전남대학교 농업생명과학대학, 동물자원학)
정영희 (전남대학교 농업생명과학대학, 동물자원학)
문승주 (전남대학교 농업생명과학대학, 동물자원학)
강승률 (농촌진흥청 난지농업연구)
강만종 (전남대학교 농업생명과학대학, 동물자원학부)
Publication Information
Reproductive and Developmental Biology / v.28, no.2, 2004 , pp. 95-99 More about this Journal
Abstract
This study was carried out to assess whether the addition of myo-inositol to maturation medium could improve porcine oocyte maturation in vitro. Oocytes were cultured for the first 22 h in Witten's medium containing 10IU/$m\ell$ PMSG, 10 IU/$m\ell$ HCG supplemented with or without myo-inositol. Subsequently, they were cultured for additional 22 h in Witten's medium without hormone supplemented with or without myo-inositol. When the porcine oocytes were cultured in maturation medium containing myo-inositol, the proportion of metaphase II oocytes 44h after culture was higher in the myo-inositol group(P<0.05). To study effects of cumulus cell on the maturation induced by myo-inositol, we examined the maturation status of cumulus-enclosed or cumulus-denuded porcine follicular oocytes. The rates of maturation were significantly higher in the cumulus-enclosed oocytes(P<0.05). However, the maturation rates of cumulus-denuded oocytes cultured in medium containing myo-inositol were higher than those of control group(P<0.05). Our results suggest that myo-inositol may affect meiotic progression of porcine follicular oocytes and supplementation of myo-inositol in maturation medium may be useful for the in vitro maturation of porcine follicular oocytes.
Keywords
Porcine oocyte; Myo-inositol; Inositol 1,4,5-triphosphate(IP3); 1,2-diacylglycerol(DAG);
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