• Journal of Applied Biological Chemistry
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• 제54권3호
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• pp.159-165
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• 2011

로즈마리 추출물의 phenol 함량은 25.7 mg/g으로 매우 높게 나타났다. Helicobacter pylori 에 대한 항균효과를 측정한 결과, clear zone이 $100{sim}200{\mu}/disc$의 phenol 농도에서 각각 11, 12, 14 mm의 clear zone을 형성하여 추출물의 농도가 증가할수록 H. pylori 에 대한 저해효과가 농도 의존적으로 높아지는 것을 확인 할 수 있었다. Sephadex LH-20과 MCI-gel CHP-20 column을 이용하여 normal phase type인 $ethanol{\rightarrow}H_2O$와 reverse phase type인 $H_2O{\rightarrow}methanol$로 용액의 농도를 증가시키면서 용출한 결과 H. pylori에 대한 탁월한 저해활성을 나타낸 5종류(compound A~E)의 단일 물질로 분리하였다. 5가지 각각의 물질은 H. pylori에 대한 항균력이 미약하였으나, 2종류 이상의 다중 물질의 항균 시너지 효과에 의해 각각 10~16 mm의 clear zone을 형성하여 우수한 항균력을 나타내었으며, fast atom bombardment (FAB)-Mass, $^1H$-NMR, $^{13}C$-NMR과 IR spectrum을 사용하여 구조 동정한 결과 protocatechuic acid, coumaric acid, caffeic acid, chlorogenic acid 및 rosmarinic acid 인 것으로 확인되었다.

• Preventive Nutrition and Food Science
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• 제3권4호
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• pp.324-328
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• 1998

We screened the methanol extracts from 133 plant species growing in Korea for antimicrobial activity against enterohemorrhagic Escherichia coli 0157 : H7. Those are selected from three plant grouping ; traditional medicinal herbs, edible plants, and flowers. They were tested by disk diffusion assay. From evaluation of the inhibition zone diameter of microbial growth, we found that the flower extract of Rhododendron Schilpenbachii Max had the most significant antimicrobial activity against this bacteria. Extracts from most of the vegetables and plants did not show antimicrobial activity except for the leaves of Ginkgo biloba L. and seeds of Prunus Dallicina L. did not show antimicrobial activity except for the leaves of Ginkgo biloba L. and the seeds of Prunus sallicina L.

• Advances in Traditional Medicine
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• 제10권1호
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• pp.44-49
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• 2010

An examination of the kinetic properties of UDP-glucose, (1,3)-$\beta$-glucans (callose) synthase, from mung bean seedlings (Sorbus commixta cortex) shows that these enzymes have a complex relationship with UDP-glucose and various effectors. Fluorescence assay showed that deoxynojirimycin increased the inhibitory effect of (1,3)-$\beta$-glucan synthase in a concentration-dependent manner. The inhibitory effect of sakuranetin (34.34%) was higher than that of deoxynojirimycin (80.63%). Disk diffusion method revealed that sakuranetin inhibited the growth of Candida albicans to a 1.5 mm inhibition zone. These results suggest that sakuranetin, isolated from Sorbus commixta cortex extract, can be used as stable antifungal material.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2001년도 추계학술발표대회
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• pp.791-795
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• 2001

This study was conducted to develop a new biomaterial to be used for skin whitening. The melanin elimination effect of chondroitin sulfate and phelinus linteus mushroom in rabbit back skin were evaluated. Rabbit dorsum was exposed to chronic UV irradiation(320nm) once daily for 30 days after initial melanin injection (100mg/kg). And then, chondroitin sulfate and phelinus linteus mushroom at dose of 0.7g for 30days were applied on the zone. The dorsal skin was histologically examined. Furthermore, we investigated free-radical extinction effect, antioxidation and tyrosinase activity inhibition effects. The histological study indicated that chondroitin sulfate and phelinus linteus mushroom decreasd melanine pigment significantly. As a result, chondroitin sulfate and phelinus linteus mushroom have a remarkable effect on the skin whitening by melanin elimination.

• 대한치과보철학회지
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• 제37권3호
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• pp.293-300
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• 1999

The purpose of this study was to evaluate the in vitro antifungal effect of several kinds of denture lining materials containing nystatin and silver-zeolite on Candida albicans. Three commercially available tissue conditioners (Soft-Liner, Coe-Comfort, Coe-Soft) and two direct denture relining materials (Tokuso Rebase, Durabase) were selected. In terms of the zone of inhibition and some basic physical properties, I could find the following results ; 1. Nystatin or silver-zeolite included in those relining materials had definite antifungal activities against the Candida albicans. 2. As times went on, both of the antifungal agents's activities decreased gradually. 3. Antifungal agents did not affect the relining materials' basic physical properties. 4 Direct chairside relining materials showed unfavorable dark discoloration with response to silver-zeolite.

• 한국식품영양과학회지
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• 제23권6호
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• pp.994-1000
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• 1994

Hot water extract from mugwort (Artemisia asiatica nakai) leaves and tea inhibited the growth of Bacillus subtilis by adding to the nutrient broth 1.0% and 0.5% concentration, respectively. Among the important compounds that contributing mugwort like flavor to the mugwort leaves and tea were considered, authenic compound of thujone, caryophyllene and farnesol showed bactericidal effect for Escherichia coli, Enterobacter aerogenes, Vibrio paraheaemolyticus, Psudomonas aeruginosa, Bacillus subtilis and Staphylococcus aureus when teste by paper disc method ( 8 mm diameter). The mixture of caryophyllene and farnesol was more bactericidal effect for various bacteria than the mixture of thujone, caryophyllene and farnesol was more bactericidal effect for various bacteria that the mixture of thujone, caryophyllene and farnesol or each compounds . Especially, the mixture of caryophyllene and farnesol showed strong bactericidal effect (diameter of inhibition zone>40mm) for Vibrio parahaemolyticus , Enterobacter aerogenes and BAcillus subtilis.

• 동의생리병리학회지
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• 제18권2호
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• pp.575-579
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• 2004

4-Geranyloxy compound (1) has elucidated by spectroscopic analysis. This compound (1) inhibited the growth of the dermatophytic fungus Trichophyton mentagrophytes ATCC 28185, (2 mm inhibition zone at 15 ㎍/disc), cytotoxic to P388 murine leukaemia eel/lines ATCC CCL 46 P388D1, (IC/sub 50/ 1,125 ng/ml at 7.5㎍/disc) and BSC monkey kidney cell lines (100% of well at 15 ㎍/disc).

• Journal of Applied Biological Chemistry
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• 제48권4호
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• pp.222-225
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• 2005

Strains showing antifungal activity against Cryphonectria parasitica were isolated from coast soil of Taean , Korea. Of 152 strains isolated, 6 strains showed antifungal activity in vitro against C. parasitica. Ta24 strain showed highest activity with 1.6 cm clean inhibition zone. For strain identification, the morphological characteristic and 16S rDNA sequences were determined. Ta24 strain showed 99% homology with Streptomyces sampsonii and was identified as Streptomyces sp.

• 한국미생물·생명공학회지
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• 제23권2호
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• pp.209-213
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• 1995

During the screening of inhibitors of melanin biosynthesis from microbial secondary metabolites, a fungal strain MR-93 which was capable of producing high level of an inhibitor was selected from plant leaf. Based on taxonomic studies, the fungus could be classified as a strain of Trichoderma sp.. The active compound (MR-93D) was purified from the culture broth by Diaion HP-20 column chromatography, ethylacetate extraction, Sephadex LH-20 column chromatography and HPLC. The inhibitor was identified as 4-hydroxy-8-isocyano-l-oxaspiro[4-4]cyclonon-8-en-2- one by spectroscopic methods of UV, $^{1}$H-NMR, ESIMS and IR. MR-93D showed a strong tyrosinase inhibitory activity with 0.03 $\mu$g/m of IC$_{50}$ value. It also inhibited melanin biosynthesis with 35 mm inhibition zone at 30 $\mu$g/paper disc in Streptomyces bikiniensis, a bacterium used as an indicator organism in this work.

• 대한약학회:학술대회논문집
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• 대한약학회 2002년도 Proceedings of the Convention of the Pharmaceutical Society of Korea Vol.2
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• pp.400.3-401
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• 2002

Levosulpiride is the levo-enantiomer from of racemic sulpride. abenzamide derivative selectively inhibition doparninergic D2 receptos at the trigger zone both in the central nervous system and in the gastrointestinal tract. We report a rapid and sensitive HPLC method using reverse phase C 18 column with fluorescence detection for separation and quantitation of levosulpiride in plasma. Tiapride was used as an internal standard. After adding an internal standard. levosulpiride in 800 ${\mu}l$ of plasma was extracted under basic conditions with ethyl acetate and methylene chloride. (omitted)