한국미생물·생명공학회지 (Microbiology and Biotechnology Letters)

  • 제23권2호
  • /
  • Pages.209-213
  • /
  • 1995
  • /
  • 1598-642X(pISSN)
  • /
  • 2234-7305(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Trichoderma sp. MR-93 균주가 생산하는 Isocyanide 계열의 Melanin 생성 저해물질

Production of the Isocyanide Inhibitor of Melanin Biosynthesis by Trichoderma sp. MR-93

  • 이충환 (한국과학기술연구원 유전공학연구소) ;
  • 전효곤 (한국과학기술연구원 유전공학연구소) ;
  • 정명철 (한국과학기술연구원 유전공학연구소) ;
  • 이호재 (한국과학기술연구원 유전공학연구소) ;
  • 배경숙 (한국과학기술연구원 유전공학연구소) ;
  • 고영희 (한국과학기술연구원 유전공학연구소)
  • 발행 : 1995.05.01
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초록

During the screening of inhibitors of melanin biosynthesis from microbial secondary metabolites, a fungal strain MR-93 which was capable of producing high level of an inhibitor was selected from plant leaf. Based on taxonomic studies, the fungus could be classified as a strain of Trichoderma sp.. The active compound (MR-93D) was purified from the culture broth by Diaion HP-20 column chromatography, ethylacetate extraction, Sephadex LH-20 column chromatography and HPLC. The inhibitor was identified as 4-hydroxy-8-isocyano-l-oxaspiro[4-4]cyclonon-8-en-2- one by spectroscopic methods of UV, $^{1}$H-NMR, ESIMS and IR. MR-93D showed a strong tyrosinase inhibitory activity with 0.03 $\mu$g/m of IC$_{50}$ value. It also inhibited melanin biosynthesis with 35 mm inhibition zone at 30 $\mu$g/paper disc in Streptomyces bikiniensis, a bacterium used as an indicator organism in this work.

키워드

참고문헌

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