Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Production of the Isocyanide Inhibitor of Melanin Biosynthesis by Trichoderma sp. MR-93

Trichoderma sp. MR-93 균주가 생산하는 Isocyanide 계열의 Melanin 생성 저해물질

  • 이충환 (한국과학기술연구원 유전공학연구소) ;
  • 전효곤 (한국과학기술연구원 유전공학연구소) ;
  • 정명철 (한국과학기술연구원 유전공학연구소) ;
  • 이호재 (한국과학기술연구원 유전공학연구소) ;
  • 배경숙 (한국과학기술연구원 유전공학연구소) ;
  • 고영희 (한국과학기술연구원 유전공학연구소)
  • Published : 1995.05.01
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Abstract

During the screening of inhibitors of melanin biosynthesis from microbial secondary metabolites, a fungal strain MR-93 which was capable of producing high level of an inhibitor was selected from plant leaf. Based on taxonomic studies, the fungus could be classified as a strain of Trichoderma sp.. The active compound (MR-93D) was purified from the culture broth by Diaion HP-20 column chromatography, ethylacetate extraction, Sephadex LH-20 column chromatography and HPLC. The inhibitor was identified as 4-hydroxy-8-isocyano-l-oxaspiro[4-4]cyclonon-8-en-2- one by spectroscopic methods of UV, $^{1}$H-NMR, ESIMS and IR. MR-93D showed a strong tyrosinase inhibitory activity with 0.03 $\mu$g/m of IC$_{50}$ value. It also inhibited melanin biosynthesis with 35 mm inhibition zone at 30 $\mu$g/paper disc in Streptomyces bikiniensis, a bacterium used as an indicator organism in this work.

Keywords

References

  1. Ann. Rev. Phytophathol. v.24 Biosynthesis and function of fungal melanin Bell,A.A.;M.H.Weeler
  2. Physiol. Rev. v.30 Biochemistry of melanin formation Lerner,A.B.;I.B.Fitzpatrick
  3. Science v.217 Mammalian tyrosinase catalyses three reactions in the biosynthesis of melanin Korner,A.;J.Pawelek
  4. Eur. J. Biochem. v.208 Regulation of final phase of melanogenesis Aroca,P.;F.Solano;C.Salinas;J.C.Gracia-Borron;J.A.Lozano
  5. J. Antibiotics v.12 A new screening method for melanin biosynthesis inhibitors using Streptomyces bikiniensis Tomit,K.N.;N.Oda;M.Kamel;T.Miyaki;T.Oki
  6. J. Antibiotics v.44 Melanostatin, a new melanin synthesis inhibitor Ishihara,Y.;M.Oka;M.Tsumakawa;K.Yomita;M.Hatori;H.Yamamoto;H.Kamei;T.Miyaki;M.Komish;T.Oki
  7. J. Antibiotics v.46 New inhibitors of melanogenesis, OH-3084 K1 and K2 Komiyama,K.;S.Takamatsu;Y.Yakahashi;M.Shinose;M.Hayashi;H.Tanaka;Y.Iwai;S.Omura
  8. Mycol. Pap. v.116 A revision of the genus Trichoderma Rifai,M.A.
  9. Composition of soil fungi Domsch,K.H.;W.Gams;T.H.Anderson
  10. 산업미생물학회지 v.21 Streptomyces sp. 20747 균주가 생산하는 tyrosinase-inhibiting isoflavonoids 이충환;전효곤;서영배;고영희
  11. J. Chem. Soc. Perkin Trans. v.1 Isolation of new isocyanide metabolites Boyd,R.K.;A.J.McAlees;A.Taylor;J.A.Walter