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Molecular methods for diagnosis of microbial pathogens in muga silkworm, Antheraea assamensis Helfer (Lepidoptera: Saturniidae)

  • Gangavarapu Subrahmanyam;Kangayam M. Ponnuvel;Kallare P Arunkumar;Kamidi Rahul;S. Manthira Moorthy;Vankadara Sivaprasad
    • International Journal of Industrial Entomology and Biomaterials
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    • v.47 no.1
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    • pp.1-11
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    • 2023
  • The Indian golden muga silkworm, Antheraea assamensis Helfer is an economically important wild silkworm endemic to Northeastern part of India. In recent years, climate change has posed a threat to muga silk production due to the requirement that larvae be reared outdoors. Since the muga silkworm larvae are exposed to the vagaries of nature, the changing climate has increased the incidence of microbial diseases in the rearing fields. Accurate diagnosis of the disease causing pathogens and its associated epidemiology are prerequisites to manage the diseases in the rearing field. Although conventional microbial culturing methods are widely used to identify pathogenic bacteria, they would not provide meaningful information on a wide variety of silkworm pathogens. The information on use of molecular diagnostic tools in detection of microbial pathogens of wild silk moths is very limited. A wide range of molecular and immunodiagnostic techniques including denaturing gradient gel electrophoresis (DGGE), random amplified polymorphism (RAPD), 16S rRNA/ITSA gene sequencing, multiplex polymerase chain reaction (M-PCR), fluorescence in situ hybridization (FISH), immunofluorescence, and repetitive-element PCR (Rep-PCR), have been used for detecting and characterizing the pathogens of insects with economic significance. Nevertheless, the application of these molecular tools for detecting and typing entomopathogens in surveillance studies of muga silkworm rearing is very limited. Here, we discuss the possible application of these molecular techniques, their advantages and major limitations. These methods show promise in better management of diseases in muga ecosystem.

Arsenic Contamination of Groundwater a Grave Concern: Novel Clay-based Materials for Decontamination of Arsenic (V)

  • Amrita Dwivedi;Diwakar Tiwari;Seung Mok Lee
    • Applied Chemistry for Engineering
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    • v.34 no.2
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    • pp.199-205
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    • 2023
  • Arsenic is a highly toxic element, and its contamination is widespread around the world. The natural materials with high selectivity and efficiency toward pollutants are important in wastewater treatment technology. In this study, the mesoporous synthetic hectorite was synthesized by facile hydrothermal crystallization of gels comprising silica, magnesium hydroxide, and lithium fluoride. Additionally, the naturally available clay was modified using zirconium at room temperature. Both synthetic and modified natural clays were employed in the removal of arsenate from aquatic environments. The materials were fully characterized by scanning electron microscope (SEM), X-ray diffraction (XRD), and Fourier transform-infrared (FT-IR) analyses. The synthesized materials were used to remove arsenic (V) under varied physicochemical conditions. Both materials, i.e., Zr-bentonite and Zr-hectorite, showed high percentage removal of arsenic (V) at lower pH, and the efficiency decreased in an alkaline medium. The equilibrium-state sorption data agrees well with the Langmuir and Freundlich adsorption isotherms, and the maximum sorption capacity is found to be 4.608 and 2.207 mg/g for Zr-bentonite and Zr-hectorite, respectively. The kinetic data fits well with the pseudo-second order kinetic model. Furthermore, the effect of the background electrolytes study indicated that arsenic (V) is specifically sorbed at the surface of these two nanocomposites. This study demonstrated that zirconium intercalated synthetic hectorite as well as zirconium modified natural clays are effective and efficient materials for the selective removal of arsenic (V) from aqueous medium.

Protocol for management of pregnant patients requiring emergency minor oral surgical procedures: a prospective study in 52 patients

  • Ajinath Nanasaheb Jadhav;Shushma G;Uzma Hamidullah Siddiqui;Minal Sharma;Yaseer Irfan Shaikh;Pooja Raosaheb Tarte
    • Journal of the Korean Association of Oral and Maxillofacial Surgeons
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    • v.49 no.1
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    • pp.21-29
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    • 2023
  • Objectives: Dental or maxillofacial emergencies are uncommon during pregnancy, but if they occur, they are challenging to treat due to potential risks. The mother should not be denied necessary medical or dental care because of pregnancy. The aim of the study is to observe outcomes of pregnancy in patients requiring emergency minor oral surgical procedures during gestation and to determine the safety of the pregnant woman undergoing the procedure and the fetus. Materials and Methods: The study was conducted on 52 pregnant women requiring emergency oral surgical procedures. A standard treatment protocol for treatment of specific entities was followed. Close monitoring and observation were the primary goal of treatment. All patients were followed postoperatively until complete recovery from the surgical procedures and then until birth of the baby. A control group of 52 healthy pregnant patients who did not require oral surgical procedures was considered for statistical analysis. The measurements to calculate observation were fetal loss (spontaneous abortion), preterm birth, low-birth weight, or incidence of any congenital anomalies in the baby and its association with surgical procedures. Results: No fetal loss occurred in any of the cases. However, four patients experienced preterm birth and seven neonates exhibited low birth weights. No congenital abnormalities were discovered. In one instance, a patient who underwent surgery for a mandibular symphysis fracture under general anesthesia in the 31st week of pregnancy experienced labor pain on the fourth postoperative day, requiring an emergency Caesarean section. Conclusion: The results of our study demonstrate that, compared to the control group, minor emergency surgeries performed during pregnancy have no discernible negative effects on the fetus. These procedures can safely be performed by adhering to our described protocols.

Biometric identification of Black Bengal goat: unique iris pattern matching system vs deep learning approach

  • Menalsh Laishram;Satyendra Nath Mandal;Avijit Haldar;Shubhajyoti Das;Santanu Bera;Rajarshi Samanta
    • Animal Bioscience
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    • v.36 no.6
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    • pp.980-989
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    • 2023
  • Objective: Iris pattern recognition system is well developed and practiced in human, however, there is a scarcity of information on application of iris recognition system in animals at the field conditions where the major challenge is to capture a high-quality iris image from a constantly moving non-cooperative animal even when restrained properly. The aim of the study was to validate and identify Black Bengal goat biometrically to improve animal management in its traceability system. Methods: Forty-nine healthy, disease free, 3 months±6 days old female Black Bengal goats were randomly selected at the farmer's field. Eye images were captured from the left eye of an individual goat at 3, 6, 9, and 12 months of age using a specialized camera made for human iris scanning. iGoat software was used for matching the same individual goats at 3, 6, 9, and 12 months of ages. Resnet152V2 deep learning algorithm was further applied on same image sets to predict matching percentages using only captured eye images without extracting their iris features. Results: The matching threshold computed within and between goats was 55%. The accuracies of template matching of goats at 3, 6, 9, and 12 months of ages were recorded as 81.63%, 90.24%, 44.44%, and 16.66%, respectively. As the accuracies of matching the goats at 9 and 12 months of ages were low and below the minimum threshold matching percentage, this process of iris pattern matching was not acceptable. The validation accuracies of resnet152V2 deep learning model were found 82.49%, 92.68%, 77.17%, and 87.76% for identification of goat at 3, 6, 9, and 12 months of ages, respectively after training the model. Conclusion: This study strongly supported that deep learning method using eye images could be used as a signature for biometric identification of an individual goat.

Prevalence and molecular analysis of glucose-6-phosphate dehydrogenase deficiency in Chin State, Myanmar

  • Ja Moon Aung;Zin Moon;Dorene VanBik;Sylvatrie-Danne Dinzouna-Boutamba;Sanghyun Lee;Zau Ring;Dong-Il Chung;Yeonchul Hong;Youn-Kyoung Goo
    • Parasites, Hosts and Diseases
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    • v.61 no.2
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    • pp.154-162
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    • 2023
  • Glucose-6-phosphate dehydrogenase (G6PD) deficiency is caused by X-linked recessive disorderliness. It induces severe anemia when a patient with G6PD deficiency is exposed to oxidative stress that occurs with administration of an antimalarial drug, primaquine. The distribution of G6PD deficiency remains unknown while primaquine has been used for malaria treatment in Myanmar. This study aimed to investigate the prevalence of G6PD deficiency and its variants in Chin State, Myanmar. Among 322 participants, 18 (11 males and 7 females) demonstrated a G6PD deficiency. Orissa variant was dominant in the molecular analysis. This would be related to neighboring Indian and Bangladeshi population, in which Orissa variant was also reported as the main mutation type. The screening test for G6PD deficiency before primaquine treatment appears to be important in Myanmar.

A study on the convergence of K-pop and world music (케이팝과 월드뮤직의 융합에 관한 연구)

  • Park, Beom-geun;Cho, Tae-seon
    • Journal of Digital Convergence
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    • v.20 no.3
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    • pp.399-406
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    • 2022
  • The purpose of this study is to increase the development possibility of K-pop music by analyzing K-pop music and analyzing the use of world music in it. As a research method, we will first look at the world music elements Puerto Rico's reggaeton, Jamaica's toasting, and the melody of Indian music. After that, we analyze two songs, Hyo-yeon's and Hyun-A & Dawn's , with rhythm and melody elements to find out the utilization of World Music. As a result of the study, it was found that it rhythmically showed the type of Dembow rhythm derived from Puerto Rico's reggaeton, and melody followed the form of Jamaican toasting and the melody of Arabic music. This study is meaningful in presenting the possibility of development and various directions of K-pop in the future by analyzing the use of world music used in K-pop music. Through the convergence of various world music and K-pop, I hope that K-pop music will become a culture that is supported by a wider public.

The Effects of Korean Exporter's Incentives on the Improvement in the Indian and ASEAN Importers' Role Performance (수출업자의 인센티브가 수입업자의 역할수행 향상에 미치는 영향: 인도와 ASEAN)

  • Choi, Chang-Bum
    • Korea Trade Review
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    • v.43 no.5
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    • pp.93-113
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    • 2018
  • The purpose of the study is to investigate whether monetary and non-monetary incentives of exports incentives affect the formation of trust between exporters and importers. Data was collected from 110 Korean SMEs(small and medium-sized enterprises) that were engaged in exporting. 'Three hypotheses were proposed and tested using the data collected. Findings include monetary incentives are not related to trust, but non-monetary incentives are positively related to trust. The findings suggest that exporters should focus on non-monetary incentives, rather than monetary incentives, to build trust with importers. Non-monetary incentives signal that exporters intend to have long-term relationship with importers, but monetary incentives do not. Trust was also found to mediate the relationship between non-monetary incentives and importer' role performance. Non-monetary incentives affect importer's role performance through trust formed between importer and exporter.

Comparative evaluation of Emblica officinalis as an etchant and an MMP inhibitor with orthophosphoric acid and chlorhexidine on the microshear bond strength of composite resin: an ex vivo study

  • Divya Sangeetha Rajkumar;Annapoorna Ballagere Mariswamy
    • Restorative Dentistry and Endodontics
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    • v.46 no.3
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    • pp.36.1-36.11
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    • 2021
  • Objectives: This study aimed to evaluate Emblica officinalis (Indian gooseberry or amla) as an acid etchant and matrix metalloproteinase (MMP) inhibitor, and to compare its effect on the microshear bond strength of composite resin with orthophosphoric acid (OPA) and 2% chlorhexidine (CHX) as an acid etchant and MMP inhibitor, respectively. Materials and Methods: The etching effect and MMP-inhibiting action of amla on dentin samples were confirmed by scanning electron microscopy (SEM) and gelatin zymography, respectively. Dentinal slabs (3 mm thick) from 80 extracted human molars were divided into 10 and 20 samples to form 2 control groups and 3 experimental groups. Groups 1, 2, and 4 were etched with OPA and groups 3 and 5 with amla juice. An MMP inhibitor was then applied: CHX for group 2 and amla extract for groups 4 and 5. Groups 1 and 3 received no MMP inhibitor. All specimens received a standardized bonding protocol and composite resin build-up, and were subjected to microshear bond strength testing. The force at which the fracture occurred was recorded and statistically analyzed. Results: Amla juice had a similar etching effect as a self-etch adhesive in SEM and 100% amla extract was found to inhibit MMP-9 by gelatin zymography. The microshear bond strength values of amla were lower than those obtained for OPA and CHX, but the difference was not statistically significant. Conclusions: Amla has a promising role as an acid etchant and MMP inhibitor, but further studies are necessary to substantiate its efficacy.

The Betrayal of Love, Trauma Narrative and Subjectivity Formation: Toni Morrison's A Mercy (사랑의 배반, 트라우마 서사와 주체 형성 -토니 모리슨의 『자비』)

  • Koo, Eunsook
    • Journal of English Language & Literature
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    • v.57 no.5
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    • pp.813-838
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    • 2011
  • Toni Morrison's ninth novel A Mercy delves into the colonial American history of the seventeenth century when Europeans began to migrate to the New World and when the first slaves were brought to Virginia. Morrison presents a diverse group of people such as white Europeans, an American Indian, a free black man, indentured servants, and slaves from Africa in order to explore the subjects of ownership, freedom and racism. She emphasizes the fact that most of the Europeans who came to America in the early seventeenth century were the people who were thrown out from the society such as felons, prostitutes, servants and children. By portraying how these castaways tried to settle in a new environment surrounded by unknown dangers and challenges, Morrison demystifies and reconstructs the myth of the birth of America as a nation state. In continuation of Morrison's writings about love and the betrayal of love, her novel A Mercy explores the subjects of trauma, memory and subjectivity by choosing the topic of motherly love and its betrayal which she dealt with poignantly in Beloved. The female protagonist, Florens, is given away by her mother in partial payment of debt incurred by the owner of Florens's mother. The traumatic memory of Florens's separation from her mother shapes Florence's character. She has to revisit the site of the original traumatic experiences of being given up by her mother in order to reconstruct her fragmented memory and past. The recurring dream of the traumatic incident that takes hold of Florens can be explained by the trauma theory of Freud, Cathy Caruth, Suzette Henke, and Judith Herman. The paper explores the self journey of Florens in which she faces the traumatic past and comprehends its meaning which enables her to construct her subjectivity by understanding the true meaning of being free and of owning oneself. In particular, it demonstrates how the process of writing a confession, a story about one's history, enables one to reclaim the traumatic experience and to locate it in the narrative memory.

Addressing the concept of Methane and Carbon emissions by wetlands and the Status of Wetlands India: A Review

  • Farheen, Kaggalu Shaista;Kim, Lee-Hyung
    • Proceedings of the Korea Water Resources Association Conference
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    • 2022.05a
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    • pp.462-462
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    • 2022
  • Wetlands are one of the most vital natural habitats on the planet. India is incredibly blessed to have a number of multifunctional wetland ecosystems. Wetlands, in addition to their functional importance, can act as sources or sinks for greenhouse gases (GHGs) depending on their intrinsic factors. Carbon (CO2) and Methane (CH4) are the major greenhouse gases (GHG's) emitted in wetlands. It is demonstrated that, despite having 4.6 percent of its area covered by natural or man-made wetlands, being home to a large number of wetlands, and being the world's second largest cultivator of paddy, India's wetlands, including paddy fields that are intermittently flooded as typical wetlands, have been very poorly studied in terms of GHG emissions. The purpose of this paper is to examine the status of Indian wetlands and wetlands in terms of CH4 and CO2 emissions. The present study also reviews various literature to provide the equations, parameters that are required for estimating carbon and methane and some of the best strategies for conserving carbon in wetlands. The findings suggest that both non-manipulative and manipulative measures can be used to improve Carbon Sequestration (CS). Non-manipulative measures aim to improve CS by increasing the spatial extent of wetlands, whereas manipulative measures aim to change the characteristics of specific wetland components that influence CS. Uncertainty in carbon dynamics projections under changing environmental conditions is caused by a number of Knowledge gaps: i) There is a lack of knowledge on how organic matter mineralizes and partitions into carbon dioxide, methane, and dissolved organic carbon, ii) With the notable exception of methane dynamics, models that represent the dynamic interaction of processes and their controls have yet to be established. As a result, more research is needed to fully understand the importance of wetlands in terms of GHG emissions and carbon sequestration in India.

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