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Isolation and Identification of Chlorella sp. CMS-1 and the Chemical Composition of Its Hot Water Extract (클로렐라 균주의 분리 동정 및 열수 추출물의 이화학적 조성)

  • Cha, Jae-Young;Kim, Jung-Wook;Park, Bo-Kyung;Jin, Hyun-Jin;Kim, Sung-Young;Cho, Young-Su
    • Journal of Life Science
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    • v.18 no.12
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    • pp.1723-1727
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    • 2008
  • Chlorella sp. CMS-1 strain was isolated from the outdoors cultivation pools in Culmansa Co., Ltd. This strain was found to be a rounded type of 3 ${\mu}m$. Phylogenetic analysis by the 18S rRNA sequencing using isolated strain is most similar to Chlorella sp. IFRPD 1018 gene at the level of nucleotide sequence identity at 99%. Accordingly, the isolated Chlorella strain was named as Chlorella sp. CMS-1 based on its morphological and phylogenetic properties. The concentrations of crude protein and fat were 59% and 0.01%, respectively. Major compositional amino acids (mg%) were glutamic acid 6.21, alanine 5.76, aspartic acid 5.44%, glycine 4.29%, and threonine 3.09% and major free amino acids (mg%) were ${\gamma}$-aminobutyric acid (GABA) 7.13%, L-alanine 1.44%, L-glutamic acid 0.90, L-leucine 0.26% and L-glycine 0.20%. The concentrations of major minerals were P 2.25%, K 2.25%, Na 1.09%, Mg 0.63%, and Ca 0.28%.

Cloning and Nucleotide Sequence Analysis of xylC Gene Encoding 5C-2HMS Dehydrogenase from Pseudomonas sp. S-47. (Pseudomonas sp. S-47로부터 5-Chloro-2-Hydroxymuconic Semialdehyde Dehydrogenase를 암호화하는 xylG 유전자의 클로닝 및 염기서열 분석)

  • Park, Song-Yi;Lee, Dong-Hoon;Kim, Young-Soo;Lee, Kyung;Kim, Chi-Kyung
    • Microbiology and Biotechnology Letters
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    • v.30 no.1
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    • pp.8-14
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    • 2002
  • Pseudomonas sp. S-47 is capable of degrading 4-chlorobenzoate to produce 5-chloro-2-hydroxymuconic semialdehyde (5C-2HMS) by the enzymes encoding by xylXYZLTE cluster. In this study, the resulting 5C-2HMS was confirmed to be transformed to 5-chloro-2-hydroxymuconic acid (5C-2HMA) by 5C-2HMS dehydrogenase. The xylG gene encoding 5C-2HMS dehydrogenase was cloned from the chromosomal DNA of strain S-47. The nucleotide sequence of xylG showed to be composed of 1,600 base pairs with ATG initiation and TGA termination codons. A deduced amino acid sequence of the 5C-2HMS dehydrogenase (XylG) exhibited 98%, 93%, and 89% identity with those of the dehydrogenases from P. putida mt-2, P. putida G7, and Pseudomonas sp. CF600, respectively.

The Recovery of the Deleted Certificate and the Detection of the Private-Key Encryption Password (삭제된 공인인증서의 복구 및 개인키 암호화 패스워드의 검출)

  • Choi, Youn-Sung;Lee, Young-Gyo;Lee, Yun-Ho;Park, Sang-Joon;Yang, Hyung-Kyu;Kim, Seung-Joo;Won, Dong-Ho
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.17 no.1
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    • pp.41-55
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    • 2007
  • The certificate is used to confirm and prove the user's identity in online finance and stocks business. A user's public key is stored in the certificate(for e.g., SignCert.der) and the private key, corresponding to public key, is stored in the private key file(for e.g., SignPri.key) after encryption using the password that he/she created for security. In this paper, we show that the certificate, deleted by the commercial certificate software, can be recovered without limitation using the commercial forensic tools. In addition, we explain the problem that the private key encryption password can be detected using the SignCert.der and the SignPri.key in off-line and propose the countermeasure about the problem.

First Report of Tomato Spotted Wilt Virus in Angelica acutiloba (당귀에서 발생한 토마토반점위조바이러스의 감염 첫 보고)

  • Kwak, Hae-Ryun;Hong, Su-Bin;Choi, Hyeon-Yong;Park, Gosoo;Hur, On-Sook;Byun, Hee-Seong;Choi, Hong-Soo;Kim, Mikyeong
    • Research in Plant Disease
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    • v.27 no.2
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    • pp.84-90
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    • 2021
  • In June 2019, Angelica acutiloba plants showing virus-like symptoms such as chlorotic local lesion and mosaic on the leaves were found in a greenhouse in Nonsan, South Korea. To identify the causal virus, we collected 6 symptomatic A. acutiloba leaf samples and performed reverse transcription polymerase chain reaction (RT-PCR) analysis using specific detection primers for three reported viruses including tomato spotted wilt virus (TSWV). RT-PCR results showed that five symptomatic samples were positive for TSWV. Mechanical sap inoculation of one of the collected TSWV isolate (TSWV-NS-AG28) induced yellowing, chlorosis and mosaic symptoms in A. acutiloba and necrotic local lesions and mosaic in Solanaceae species. Phylogenetic analysis based on the complete genome sequences showed that TSWV-NS-AG28 had a maximum nucleotide identity with TSWVNS-BB20 isolated from butterbur in Nonsan, South Korea. To our knowledge, this is the first report of TSWV infection in A. acutiloba.

Physiology and Growth Properties of Halophilic Bacteria Isolated from Jeotgal (Salted Seafood) (젓갈(염장발효식품)에서 분리한 호염세균의 생리 및 성장특성)

  • Jung Yoo Jeong;Park Doo Hyun
    • Korean Journal of Microbiology
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    • v.40 no.4
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    • pp.263-268
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    • 2004
  • Two species of halophilic bacteria were isolated from five salted seafoods and identified by 16S rDNA sequenc­ing homology. One was identified as Halomonas subglaciescola and other four strains were belong to Halomo­nas marina. The identity of all isolates with standard organisms was above $95\%.$ H. subglaciescola, H. marina IN, and H. marina SH-2 grew in salinity condition from $3%\;to\;18\%$ NaCl but growth of H. marina SQ and H. marina SH-l grew in salinity environment from $8\%\;to\;17\%.$ Maximum biomass of H. subglaciescola, H. marina IN, H. marina SQ, H. marina SH-1, and H. marina SH-2 growing in LB medium containing $15\%$ NaCl were about 3.2, 4.5, 4.5, 5.7, and 4.2, however the maximum biomass in LB medium containing $5\%$ NaCl were about 2.2, 1.1, 0.7, 0.2, and 2.4 as optical density at 660 nm, respectively. In scanning electron micrograph, unknown material (mucus) attached to outer membrane of all isolates was observed. When mucus isolated from halophilic bacterial cell was added to culture of E. coli, E. coli grew in medium containing $15\%$ NaCl.

A Study of Male Luxury Consumption and the Cultural Politics of Difference Focused on the Consumer Experiences of Men in their 30s (남성의 명품 소비와 차이의 문화정치 한국 사회 30대 남성의 소비 경험을 중심으로)

  • Ryoo, Woongjae;Park, Jeongeun
    • Korean journal of communication and information
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    • v.75
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    • pp.9-42
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    • 2016
  • This study critically examines the social and personal meanings that luxury consumption have in contemporary Korean society through understanding the consumption experiences of men in their 30s. Interviews with nine male consumers who are committed to luxury brands, especially those that produce suits, watches, fountain pens, and shoes, were conducted to collect qualitative data. We found that participants are satisfied with the whole process of luxury brand consumption including buying, using, and managing and try to consume the products for self-expression, congruent with their lifestyle and identity. Individuals have learned how to consume the luxury brand from their role models. Their consumption follows hybrid and postmodern patterns and combines luxury brand products with non-luxury one. In contemporary Korean society, using the luxury products is considered necessary to achieve some advantages in social contexts and can be also functioned as a sort of social signifier and self-help, as well as, a tool for self satisfaction or well-being.

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A Study on the Development of Career Program for Senior Student-Athletes in College (대학 졸업을 앞둔 학생선수의 진로준비과정을 위한 진로프로그램 개발 연구)

  • Lee, Taerim;Park, Soungjuu;Kim, Kyung Min;Lee, Bora
    • Korean Journal of School Psychology
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    • v.18 no.3
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    • pp.371-398
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    • 2021
  • Student-athletes identify themselves as athletes first and students second and experience identity foreclosure in which they rarely consider other possibilities for their career besides professional athletes. There is a gap between the career education that college student-athletes and regular students get, so we conducted this study to test the effectiveness of a career education program. The proposed career education program was based on Super and Ginsberg's career development theory and Holland's career choice theory. Data were collected from 13 college student-athletes using a pretest-posttest research design and analyzed using a t-test. The program was conducted in the form of 90-minute weekly sessions over 3 weeks. The program's effectiveness was measured in terms of participants' stress from a lack of career information, career preparedness, self-understanding, and career decision-making self-efficacy. The results showed that each variable, except stress from a lack of career information, was significantly improved after the program compared to before the program, indicating that the program was effective. Theoretical and practical implications regarding career education for student-athletes are discussed in this paper.

IMC Strategy of Sinhan Card for Building the Strong Brand Equity (신한카드 브랜드구축을 위한 IMC전략)

  • Ahn, Kwang Ho;Yoo, Chang Jo;Park, Woon Yong
    • Asia Marketing Journal
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    • v.13 no.3
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    • pp.249-264
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    • 2011
  • Brand is the major enduring asset of a company and more valuable than the totality of specific products and facilities. Thus brands are powerful assets that must be carefully developed and managed. The company should design and implement marketing activities and marketing communication programs to build and grow the brand value. Strarbucks, Samsung Galaxy, and Nike brands get a price premium and generate strong customer loyalty. A power brand has high brand equity, which is the differential effect that brand knowledge has on consumer response to the marketing of that brand. Brand equity is created when brands have strong, favorable and unique brand associations with customers and high level of brand awareness. Therefore marketers in building a strong brand should ensure to develop the right type of customer experiences with products and effective integrated marketing communication(IMC) programs to create the brand equity. Since 2007 Sinhan card acquiring the LG card has developed the new brand identity and carefully managed the advertising campaign and other marketing communication mix tools to create the high brand awareness and differential brand image. In this case study we examine how Sinhan card with the goal of being No.1 brand in the credit card market has developed and implemented the IMC Strategy to build a high level of consumer brand awareness, unique brand image and strong customer relationship.

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Does Social Exclusion Increase Materialism? The Moderated Mediation Model of the Need to Belong, the Need for Control, and a Sense of Power (사회적 배제는 물질주의를 증가시키는가? 소속과 통제의 욕구 및 권력감의 조절된 매개 효과)

  • Hyorim Chung;Hyebin Kwon;Jiyoung Park
    • Science of Emotion and Sensibility
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    • v.26 no.3
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    • pp.53-68
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    • 2023
  • Materialism can be triggered by various social events. Based on theories on functions of materialistic goal pursuit, this study investigates how social exclusion evokes desires related to one's identity, resulting in an increase in materialism. Specifically, we predicted that social exclusion would increase the desire for control and belonging, thus leading to higher levels of materialism. Moreover, based on the theory of the approach tendency of power, we further predicted that individuals with a higher sense of power would experience greater desires for belonging and control when faced with social exclusion. To examine the hypotheses, we conducted an experiment using 202 Korean women. The results indicated that social exclusion resulted in an increase in the desire for control, thus increasing materialism. This relationship is stronger for individuals with a high sense of power, and the path from social exclusion to materialism based on the need for control is significant for those with a high sense of power. Although social exclusion increases the need to belonging, the relationship between the need to belonging and materialism is not significant, and its mediating effect was not supported in this study. Based on these findings, we discussed implications and directions for future research.

A Study on Vulnerability Prevention Mechanism Due to Logout Problem Using OAuth (OAuth를 이용한 로그아웃 문제로 인한 취약점 방지 기법에 대한 연구)

  • Kim, Jinouk;Park, Jungsoo;Nguyen-Vu, Long;Jung, Souhwan
    • Journal of the Korea Institute of Information Security & Cryptology
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    • v.27 no.1
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    • pp.5-14
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    • 2017
  • Many web services which use OAuth Protocol offer users to log in using their personal profile information given by resource servers. This method reduces the inconvenience of the users to register for new membership. However, at the time a user finishes using OAuth client web service, even if he logs out of the client web service, the resource server remained in the login state may cause the problem of leaking personal information. In this paper, we propose a solution to mitigate the threat by providing an additional security behavior check: when a user requests to log out of the Web Client service, he or she can make decision whether or not to log out of the resource server via confirmation notification regarding the state of the resource server. By utilizing the proposed method, users who log in through the OAuth Protocol in the public PC environment like department stores, libraries, printing companies, etc. can prevent the leakage of personal information issues that may arise from forgetting to check the other OAuth related services. To verify our study, we implement a Client Web Service that uses OAuth 2.0 protocol and integrate it with our security behavior check. The result shows that with this additional function, users will have a better security when dealing with resource authorization in OAuth 2.0 implementation.