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Cloning and Nucleotide Sequence Analysis of xylC Gene Encoding 5C-2HMS Dehydrogenase from Pseudomonas sp. S-47.  

Park, Song-Yi (Department of Microbiology, Chungbuk National University)
Lee, Dong-Hoon (Department of Microbiology, Chungbuk National University)
Kim, Young-Soo (Department of Pharmacy,Chungbuk National University)
Lee, Kyung (Department of Microbiology, Changwon National University)
Kim, Chi-Kyung (Department of Microbiology, Chungbuk National University)
Publication Information
Microbiology and Biotechnology Letters / v.30, no.1, 2002 , pp. 8-14 More about this Journal
Abstract
Pseudomonas sp. S-47 is capable of degrading 4-chlorobenzoate to produce 5-chloro-2-hydroxymuconic semialdehyde (5C-2HMS) by the enzymes encoding by xylXYZLTE cluster. In this study, the resulting 5C-2HMS was confirmed to be transformed to 5-chloro-2-hydroxymuconic acid (5C-2HMA) by 5C-2HMS dehydrogenase. The xylG gene encoding 5C-2HMS dehydrogenase was cloned from the chromosomal DNA of strain S-47. The nucleotide sequence of xylG showed to be composed of 1,600 base pairs with ATG initiation and TGA termination codons. A deduced amino acid sequence of the 5C-2HMS dehydrogenase (XylG) exhibited 98％, 93％, and 89％ identity with those of the dehydrogenases from P. putida mt-2, P. putida G7, and Pseudomonas sp. CF600, respectively.
Keywords
xylG; Cloning and sequencing; 5C-2HMS; Pseudomonas sp. S-47;
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