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http://dx.doi.org/10.5423/RPD.2021.27.2.84

First Report of Tomato Spotted Wilt Virus in Angelica acutiloba  

Kwak, Hae-Ryun (Crop Protection Division, National Institute of Agricultural Sciences)
Hong, Su-Bin (Crop Protection Division, National Institute of Agricultural Sciences)
Choi, Hyeon-Yong (Crop Protection Division, National Institute of Agricultural Sciences)
Park, Gosoo (Nonsan Agricultural Technology Center)
Hur, On-Sook (National Agrobiodiversity Center, National Institute of Agricultural Sciences)
Byun, Hee-Seong (Crop Protection Division, National Institute of Agricultural Sciences)
Choi, Hong-Soo (Crop Protection Division, National Institute of Agricultural Sciences)
Kim, Mikyeong (Department of Plant Medicine, Chungbuk National University)
Publication Information
Research in Plant Disease / v.27, no.2, 2021 , pp. 84-90 More about this Journal
Abstract
In June 2019, Angelica acutiloba plants showing virus-like symptoms such as chlorotic local lesion and mosaic on the leaves were found in a greenhouse in Nonsan, South Korea. To identify the causal virus, we collected 6 symptomatic A. acutiloba leaf samples and performed reverse transcription polymerase chain reaction (RT-PCR) analysis using specific detection primers for three reported viruses including tomato spotted wilt virus (TSWV). RT-PCR results showed that five symptomatic samples were positive for TSWV. Mechanical sap inoculation of one of the collected TSWV isolate (TSWV-NS-AG28) induced yellowing, chlorosis and mosaic symptoms in A. acutiloba and necrotic local lesions and mosaic in Solanaceae species. Phylogenetic analysis based on the complete genome sequences showed that TSWV-NS-AG28 had a maximum nucleotide identity with TSWVNS-BB20 isolated from butterbur in Nonsan, South Korea. To our knowledge, this is the first report of TSWV infection in A. acutiloba.
Keywords
Angelica acutiloba; Orthotospovirus; Tomato spotted wilt virus;
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