• Fisheries and Aquatic Sciences
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• v.9 no.2
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• pp.75-82
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• 2006

We evaluated the utility of applying isozyme analysis and two tagging methods, visible implant fluorescent elastomer (VIE) injection and uropod-cutting, to monitor the effects of releasing nursery-reared fleshy prawn (Fenneropenaeus chinensis) into natural habitat in Korea. One hundred thousand farmed prawns (70 mm long) were tagged by clipping off the outer left uropod and injecting them with VIE. This marked seed population was released at Muchangpo, Korea, on 11 and 19 July 2002. Two months later, total catch and catch per unit effort (CPUE) at three locations (Hongwon, Muchangpo, and Anmyundo) were determined. Total catch and CPUE increased nearly 18% over the previous year in Hongwon and Muchangpo. The mixing rate, estimated by uropod regeneration pattern, was 0.33% at Hongwon, 0.53% at Muchangpo, and 0.21% at Anmyundo. The recapture rate was about 3.5%. Isozyme analysis confirmed that the mixing rate was highest at Muchangpo. Moreover, fleshy prawns from Muchangpo were genetically most related to the seed population, indicating that the released prawns had largely remained near the released site. We also confirmed that isozyme genes are valuable as genetic markers for qualitative analyses of a released seed population.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.31 no.3
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• pp.375-382
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• 1986

This study was carried out to obtain the basic information for the clarification of spring growth habits mechanism of naked barleys. The isozyme patterns and activities of peroxidase in the young spike and leaf blade were analyzed during the differentiation and development of young spike. The characteristic differences between the normal and rosetted type were in c and g isozymes in young spike, and in i isozyme in the leaf blade. In the normal type, c and i isozymes disappeared at the stage of spi-kelet differentiation, g isozyme at the stage of flolet differentiation. But, in the rosetted type, those three isozymes remained in dark stained condition until the time of final sampling. Especially, those three isozymes were higher in the rosetted type than those in the normal type even at the stage of bract differentiation(BDS), just prior to the reproductive stage. The activities of peroxidase decreased slowly after BDS in the young spike and leaf blade in the normal type, While, in the rosetted type, increased linearly, and the degree of increasing was remarkable in the young spike. It was interesting that the degree of activities in young spike was higher in the rosetted type than that in the normal type even at BDS. From the above results, the remarkable differences of the isozyme patterns and activities at BDS between the normal and rosetted type were considered to be the physiological expression of the varieties concerned with the degree of spring growth habits.

• Journal of Plant Biology
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• v.38 no.1
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• pp.47-54
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• 1995

The present study performed the isolation of cytosolic ascorbate peroxidase (APX) isozymes and analyzed the pattern of their activity development and also investigated the change in some other enzyme activities related to the ascorbate-glutathione pathway from the senescing wheat leaves. The aim of this work is to examine the possibility that in the cytoplasm of wheat leaves the ascorbate-glutathione pathway p!ays a significant role in relation to leaf senescence involving an $H_2O_2$ accumulation and then to show the effect of benzyladenine (BA) on that pathway. During the leaf senescence characterized by increases in ChI breakdown and H202 accumulation under the 4-day dark incubation of matured leaf segments; i) no significant increase of total cytosolic APX was observed, ii) a dehydroascorbate reductase (DHAR) activity was decreased rapidly, iii) a slight increase of glutathione reductase (GR) activity occurred. In the BA-treated leaves; however, i) the total activity of APX increased conspicuously, ii) the decrease of DHAR activity was relatively inhibited, iii) the GR activity increase was more enhanced, and iv) the decrease of ascorbate content and the increase of H202 content were retarded as compared with those of control leaves. Three isozymes of cytosolic APX were found by using a native-electrophoretic gel in senescing wheat leaves and two of them occurred with major activity. In the developmental patterns of cytosolic APX isozymes, only two isozyme bands ("a" and "b") appeared with almost constant activity through 4 days of incubation in the control leaves, while one additional weak isozyme band ("c") and a little increase of "b" isozyme activity were detected in the BA-treated leaves. EspeciaUy, the development of "a" isozyme activity increased remarkably compared with that of control leaves. The increased capacity for peroxide scavenging due to the enhanced activity of all 3 enzymes (APX, DHAR, GR) participating in the ascorbate-glutathione pathway in BA-treated leaves suggested that this pathway might playa significant role in the processes related to the wheat leaf senescence.scence.

• YAKHAK HOEJI
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• v.19 no.1
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• pp.36-46
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• 1975

In the rats administered mercuric chloride (1mg/kg/48 hrs i.p.), it was found that LDH$_{1}$ and LDH$_{2}$ were increased in heart, brain and kidney as well as LDH$_{5}$ increased in liver and muscle in the duration of sixteen days. After the sixteen days of administration, the LDH isozyme patterns in the above mentioned organs were found to be irregularly changed. Considerable amount of mercury accumulation in liver and kidney were found, and especially the mercury accumulation in kidney was notable.

• The Korean Journal of Ecology
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• v.21 no.6
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• pp.771-777
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• 1998

Aqueous extracts of Pinus rigida changed the electrophoretic patterns of total proteins and of hydrolytic enzymes such as peroxidase, esterase and amylase during the germination of radish (Raphanus sativus var. hortensis for. acanthiformis). When the extract treatment was finished, at the late stage of radish germination, aqueous extracts of P. rigida had suppressed the expression of 24 KD and 60 KD proteins. the extract induced new isozyme bands, indicating concomitant activity of peroxidases, esterase activities were stimulated in the cathodic region. The activity of amylase was enhanced by the extract.

• Animal cells and systems
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• v.3 no.2
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• pp.133-141
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• 1999

To examine the taxonomic position and affinities of Isopyrum mandshuricum (Ranunculaceae) and related taxa, genetic analysis were carried out on the basis of isozyme patterns and ITS sequences. Molecular data, both isozyme patterns and ITS sequences suggest that I. mandshuricum is closely related to Enemion raddeanum than to Semiaquilegia adoxoides. The estimation of genetic identities by isozyme analysis reveals that I. manshuricum is genetically distant from E. raddeanum. The phylogenetic tree based on molecular data is rather congruent with the phenogram based on quantitative morphological characteristics, but not consistent with one based on qualitative morphological characteristics. Incongruencies between molecular and qualitative morphological data provide clues to re-evaluate several morphological features.

• The Korean Journal of Mycology
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• v.27 no.5 s.92
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• pp.328-336
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• 1999

Isozyme comparisons of mycelial extracts from Pleurotus ostreatus were undertaken using isoelectric focusing. Enzyme isozyme patterns were Used to describe the extent of geographical diversity and degree of intraspecific variation in these extracts. A total of 77 bands were resolved from six different enzymes. Cluster analyses were performed using the zymograms for esterase (EST), glucose phosphate isomerase (GPI), leucine aminopeptidase (LAP), malate dehydrogenase(MDH), peroxidase (POX), and phosphoglucomutase (pGM). EST gave multiple banding patterns, while less variability was observed for GPI, MDH, and PGM. Cluster analyses demonstrated that strains of P. ostreatus from geographically different origins are genetically divergent, supporting the idea that there is little or no gene flow between these geographically distant population groups.

• Journal of Plant Biotechnology
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• v.4 no.1
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• pp.39-44
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• 2002

The fast-migrating cationic peroxidase isozyme, named RC3, was purified from rice (Oryza sativa cv. Nak-Dong) callus. Purification of the enzyme was accomplished by ammonium sulfate fractionation, CM-cellulose ionexchange chromatography, and Sephacryl S-100 gel filtration. The molecular mass of the enzyme was about 34 KDa as determined by SDS-PACE and 38 KDa by Sephacryl-100 gel filtration. The pI value of the enzyme was 8.9. Antiserum against RC3 was raised in rabbits, and anti RC3 antiserum reacted with RC3 isozyme by Ouchterlony double immunodiffusion. The optimum pHs and Km values of the enzyme for various substrates were determined. Kinetic studies with various substrates showed that RC3 had very low Km value of 0.01 mM for ferulic acid and ascorbic acid. However, the enzyme did not use esculetin as a substrate.

• Korean Journal Plant Pathology
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• v.14 no.5
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• pp.502-506
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• 1998

In this study, we calculated the genetic relationships of Pestalotiopsis species collected from various places in southern part of Korea through isozyme analyses. As a result, EST showed the largest number of band, and the number of bands were ranged from 5 to 7 on the average. All the isozymes used in this study showed distinctive band patterns for each isolates. Similarities among the compared isolates ranged from 48 to 93%. Isolates SP7, SP19 and SP23 showed more than 90% similarities, and most isolates showed similarities ranging from 65 to 82%.

• YAKHAK HOEJI
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• v.20 no.1
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• pp.92-96
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• 1976

A series of experimental groups has been studied in the state of hypolycemia caused by a single intraperitoneal injection of insulin after 24 hrs of fasting albino rats and then the variation of LDH activities and LDH isozyme patterns in the liver, muscle and serum had been reported. The total LDH activity has been elevated in the liver and the muscle above the average level for control group, but increased continusly during 20 min and decreased in the 20-45 min intervals and increased again 45 min in the serum. The change of LDH isozyme patterns had been shown that in the liver LDH$_{5}$ was increased, LDH$_{4}$ was decreased and in the muscle LDH$_{1}$ was diminished by 30 min ws restored again after 45 min and LDH$_{2}$ decreased about 94 percentage at 30 min, decreased by 45 min and increased greater again after 45 min and in the muscle LDH$_{3}$, LDH$_{4}$, and LDH$_{5}$ were increased to the greatest by 20 min, decreased in 20-45 min intervals and increased again after 45 min.