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http://dx.doi.org/10.5657/fas.2006.9.2.075

Utility of Isozyme as a Genetic Marker for Estimating the Effects of Release and Stock Enhancement of Fleshy Prawn Fenneropenaeus chinensis  

Han Hyon-Sob (Biotechnology Research Center, National Fisheries Research and Development Institute)
Jang In-Kwon (Crustacean Research Center)
Jun Je-Cheon (West Sea Fisheries Research Institute)
Kim Jong-Hwa (Inland Fisheries Ecological Research Institute)
Park Young-Je (West Sea Fisheries Research Institute)
Publication Information
Fisheries and Aquatic Sciences / v.9, no.2, 2006 , pp. 75-82 More about this Journal
Abstract
We evaluated the utility of applying isozyme analysis and two tagging methods, visible implant fluorescent elastomer (VIE) injection and uropod-cutting, to monitor the effects of releasing nursery-reared fleshy prawn (Fenneropenaeus chinensis) into natural habitat in Korea. One hundred thousand farmed prawns (70 mm long) were tagged by clipping off the outer left uropod and injecting them with VIE. This marked seed population was released at Muchangpo, Korea, on 11 and 19 July 2002. Two months later, total catch and catch per unit effort (CPUE) at three locations (Hongwon, Muchangpo, and Anmyundo) were determined. Total catch and CPUE increased nearly 18% over the previous year in Hongwon and Muchangpo. The mixing rate, estimated by uropod regeneration pattern, was 0.33% at Hongwon, 0.53% at Muchangpo, and 0.21% at Anmyundo. The recapture rate was about 3.5%. Isozyme analysis confirmed that the mixing rate was highest at Muchangpo. Moreover, fleshy prawns from Muchangpo were genetically most related to the seed population, indicating that the released prawns had largely remained near the released site. We also confirmed that isozyme genes are valuable as genetic markers for qualitative analyses of a released seed population.
Keywords
Fleshy prawn; Genetic marker; Isozyme; Recapture rate; Release effect; Fenneropenaeus chinensis;
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