Browse > Article

Enzymatic Properties of Fast-migrating Cationic Peroxidase Isozyme from Rice Callus  

Yoo, Kyung-A (Department of Life Science, College of Science, Soonchunhyang University)
Lee, Mi-Young (Department of Life Science, College of Science, Soonchunhyang University)
Publication Information
Journal of Plant Biotechnology / v.4, no.1, 2002 , pp. 39-44 More about this Journal
Abstract
The fast-migrating cationic peroxidase isozyme, named RC3, was purified from rice (Oryza sativa cv. Nak-Dong) callus. Purification of the enzyme was accomplished by ammonium sulfate fractionation, CM-cellulose ionexchange chromatography, and Sephacryl S-100 gel filtration. The molecular mass of the enzyme was about 34 KDa as determined by SDS-PACE and 38 KDa by Sephacryl-100 gel filtration. The pI value of the enzyme was 8.9. Antiserum against RC3 was raised in rabbits, and anti RC3 antiserum reacted with RC3 isozyme by Ouchterlony double immunodiffusion. The optimum pHs and Km values of the enzyme for various substrates were determined. Kinetic studies with various substrates showed that RC3 had very low Km value of 0.01 mM for ferulic acid and ascorbic acid. However, the enzyme did not use esculetin as a substrate.
Keywords
fast-migrating cationic peroxidase isozyme; properties; purification; rice callus;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Heavy metal-induced changes in peroxidase activity in leaves, roots and cell suspension cultures of Hordeum vulgare L. /
[ Antje B;Ahmed A;Marina A;Andreas B;Karl-Josef D;Obinger C(ed.);Burner U(eds.;Ebermann R(ed.);Penel C(ed.);Creppin H(ed.) ] / Plant Peroxidases: Biochemistry and Physiology
2 Plant peroxidases: Their primary, secondary and tertiary structures and relation to cytochrome C peroxidase /
[ Welinder KG ] / Eur J Biochem   DOI   ScienceOn
3 Heme peroxidase nomenclature /
[ Dunford HB;Penel C(ed.);Gaspar T(ed.);Greppin H(ed.) ] / Plant peroxidase news letter: Plant Biochemistry and Physiology
4 Molecular characterization of cDNAs for two anionic peroxidases from suspension cultures of sweet potato /
[ Kim KY;Huh GH;Lee HS;Kwon SY;Hur Y;Kwak SS ] / Mol Gen Genet   DOI   ScienceOn
5 Purification and characterization of a cationic isoperoxidase from scented-geranium callus /
[ Lee SH;Kim, ES;Lee MY ] / Phytochemistry   DOI   ScienceOn
6 Molecular cloning and characterization of cDNAs for anionic and neutral peroxidases from suspension-cultured cells of sweet potato and their differential expression in response to stress /
[ Huh GH;Lee SJ;Bae YS;Liu JR;Kwak SS ] / Mol Gen Genet
7 Some molecular aspects of plant peroxidase biosynthetic studies /
[ van Huystee RB ] / Annu Rev Plant Physiol   DOI
8 Extracellular biochemical markers of photochemical oxidant air pollution damage to Norway spruce /
[ Castillo FJ;Miller PR;Greppin H ] / Experientia   DOI
9 A large family of Class III plant peroxidases /
[ Hiraga S;Sasaki K;Ito H;Ohashi Y;Matsui H ] / Plant Cell Physiol
10 Characteristics of six isoperoxidases from Korean radish root /
[ Lee MY;Kim SS ] / Phytochemistry
11 Glycoproteins of cell surfaces /
[ Grossman H;Neville DN ] / J Biol Chem
12 Environmental stress-induced extracellular isoperoxidase RC3 from rice /
[ Yi KW;Yoo KA;Lee MY ] / J Environ Biol
13 Progress and prospects in the use of peroxidase to study cell development /
[ van Huystee RB;Cairns WL ] / Phytochemistry   DOI   ScienceOn
14 Diverse expression profiles of 21 rice peroxidase genes /
[ Hiraga S;Yamamoto K;Ito H;Sasaki K;Matsui H;Honma M;Nagamura Y;Sasaki T;Ohashi Y ] / FEBS Lett   DOI
15 cDNA cloning and overexpression of an isoperoxidase gene from Korean radish, Raphanus sativus /
[ Park JH;Kim SS ] / J Biochem Mol Biol   과학기술학회마을
16 Rice cationic peroxidase accumulates in xylem vessels during incompatible in interactions with Xanthomonas ozyae pvoryzae /
[ Scott AY;Ailan G;James AG;Frank FW;Jan EL ] / Plant Physiol   DOI
17 Protein measurement with the folin phenol reagent /
[ Lowry OH;Rosebrough NJ;Farr AL;Randall RJ ] / J Biol Chem
18 Carbohydrate moieties of three radish peroxidases /
[ Kim SH;Kim SS ] / Phytochemistry   DOI   ScienceOn
19 Purification and characterization of rice peroxidase /
[ Ito H;Hiraoka N;Ohbayashi A;Ohashi Y ] / Agri Biol Chem   DOI   ScienceOn
20 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 /
[ Laemmli UK ] / Nature   DOI   ScienceOn
21 A genetic and enzymatic survey of Arabidopsis thaliana peroxidases /
[ Tognolli M;Overney S;Penel C;Greppin H;Simon P;Penel C(ed.);Gaspar T(ed.);Greppin H(ed.) ] / Plant peroxidase newsletter: Plant Biochemistry and Physiology
22 Mechanisms of insect resistance in transgenic plants (over)expressing a tobacco anionic peroxidase /
[ Dowd PF;Herms DA;Berhow MA;Lagrimini LM;Penel C(ed.);Gaspar T(ed.);Greppin H(ed.) ] / Plant peroxidase news letter: Plant Biochemistry and Physiology
23 Reaction of peroxidase from different plant species to increased temperatures and the effect of calcium and zinc ions /
[ Bakardjieva NT;Christova NV;Christov K;Obinger C(ed.);Burner U(ed.);Ebermann R(ed.);Penel C(ed.);Greppin H(ed.) ] / Plant Peroxidases: Biochemistry and Physiology
24 Chilling-enhanced photooxidation. Evidence for the role of singlet oxygen and super oxide in the breakdown of pigments and endogenous antioxidants /
[ Wise RR;Naylor AW ] / Plant Physiol
25 Purification and immunological relationships of six radish isoperoxidases /
[ Lee MY;Choi Y;Kim SS ] / Plant Physiol Biochem
26 Inactivation and cleavage of radish peroxidase by various reducing agents /
[ Lee MY;Kim SS ] / Phytochemistry   DOI   ScienceOn
27 Evidence for the chiling-induced oxidative stress in Maize seedling and a regulatory role for hydrogen peroxide /
[ Prasad TK;Anderson MD;Martin BA;Sewart CR ] / The Plant Cell   DOI   ScienceOn
28 Effects of <TEX>$Na_2SO_3$</TEX> on the activities of antioxidant enzymes in geranium seedlings /
[ Lee MY ] / Phytochemistry   DOI   ScienceOn