DOI QR코드

DOI QR Code

Utility of Isozyme as a Genetic Marker for Estimating the Effects of Release and Stock Enhancement of Fleshy Prawn Fenneropenaeus chinensis

  • Han Hyon-Sob (Biotechnology Research Center, National Fisheries Research and Development Institute) ;
  • Jang In-Kwon (Crustacean Research Center) ;
  • Jun Je-Cheon (West Sea Fisheries Research Institute) ;
  • Kim Jong-Hwa (Inland Fisheries Ecological Research Institute) ;
  • Park Young-Je (West Sea Fisheries Research Institute)
  • Published : 2006.06.01

Abstract

We evaluated the utility of applying isozyme analysis and two tagging methods, visible implant fluorescent elastomer (VIE) injection and uropod-cutting, to monitor the effects of releasing nursery-reared fleshy prawn (Fenneropenaeus chinensis) into natural habitat in Korea. One hundred thousand farmed prawns (70 mm long) were tagged by clipping off the outer left uropod and injecting them with VIE. This marked seed population was released at Muchangpo, Korea, on 11 and 19 July 2002. Two months later, total catch and catch per unit effort (CPUE) at three locations (Hongwon, Muchangpo, and Anmyundo) were determined. Total catch and CPUE increased nearly 18% over the previous year in Hongwon and Muchangpo. The mixing rate, estimated by uropod regeneration pattern, was 0.33% at Hongwon, 0.53% at Muchangpo, and 0.21% at Anmyundo. The recapture rate was about 3.5%. Isozyme analysis confirmed that the mixing rate was highest at Muchangpo. Moreover, fleshy prawns from Muchangpo were genetically most related to the seed population, indicating that the released prawns had largely remained near the released site. We also confirmed that isozyme genes are valuable as genetic markers for qualitative analyses of a released seed population.

Keywords

References

  1. Ariyama, H., F. Uratani, H. Ohyama, M. Sano and S. Yamochi. 1994. Survival, growth, and tag retention of the kuruma prawn Penaeus japonicus and the greasy back prawn Metapenaeus ensis injected with gold bit tags. Fish. Sci., 60, 785-786 https://doi.org/10.2331/fishsci.60.785
  2. Birt, T.P., J.M. Green and W.S. Davidson. 1991. Mitochondrial DNA variation reveals genetically distinct sympatric populations of anadromous and non-anadromous Atlantic salmon, Salmo salar. Can. J. Fish. Sci., 48, 577-582 https://doi.org/10.1139/f91-073
  3. Campton, D.E. and J.M. Johnston. 1985. Electrophoretic evidence for a genetic admixture of native and non-native rainbow trout in the Yakima River, Washington. Trans. Am. Fish. Soc., 114, 782-793 https://doi.org/10.1577/1548-8659(1985)114<782:EEFAGA>2.0.CO;2
  4. Farmer, A. 1981. A review of crustacean marking methods with particular reference to penaeid shrimp. Kuwait Bull. Mar. Sci., 2, 167-183
  5. FCDAF (Fukuoka City Division of Agriculture & Fisheries). 1987. The report on release effect of kuruma prawn in Hakada Bay. Fukuoka City Division of Agriculture & Fisheries, Fukuoka, Japan
  6. Harada, Y. 1992. Genetic difference between wild and released individuals and the resource enhancement effect of stocking: a theoretical analysis. Nippon Suisan Gakkaishi, 58, 2269-2275 https://doi.org/10.2331/suisan.58.2269
  7. Hedgecock, D., M.L. Tracey and K. Nelson. 1982. Genetics. In: L. G. Abelle and D. E. Bliss (Eds.) The Biology of Crustacea, Vol. 2. Academic Press, New York, 283-403
  8. Holthuis, L.B. 1980. FAO species catalogue. Vol. 1. Shrimps and prawn of the world. An annotated catalogue of species of interest to fisheries. FAO Fisheries Synopsis, 125, 1-261
  9. Hwang, G.L. 1996. Stock characterization of the fleshy prawn (Penaeus chinensis) in the Yellow Sea by intraspecific sequence variation of the cytochrome c oxidase subunit I gene. J. Kor. Fish. Soc., 29, 876-881
  10. Hwang, G.L., Y.C. Lee and C.S. Chang. 1997. Mitochondrial DNA analysis of the fleshy prawn (Penaeus chinensis) for stock discrimination in the Yellow Sea. J. Kor. Fish. Soc., 30, 88-94
  11. Kim, B.K. and K.H. You. 1989. Studies on the seed production and cultivation of fleshy prawn, Penaeus chinensis (Osbeck, 1765). Bull. Natl. Fish. Res. Dev. Agency, 43, 119-125
  12. Miyajima, T., Y. Hamanaka and K. Toyota. 1999. A marking method for kuruma prawn Penaeus japonicus. Fish. Sci., 65, 31-35 https://doi.org/10.2331/fishsci.65.31
  13. Morikawa, M., S. Arimoto, H. Takemoto and Y. Yoshii. 1996. Mass release and recapture of kuruma prawn by using corded wire tag. Abstract of the 8th Annual Meeting of the Japanese Society of Fisheries Science, Tokyo, Japan
  14. Sato, R., K. Naka and R. Ishida. 1982. Application of isozyme as a genetic marker for fish farming. Fish Genet. Breed. Sci., 7, 1-8
  15. Seki, S. and N. Taniguchi. 1988. Tracking the released ayu of landlocked form by isozyme markers. Nippon Suisan Gakkaishi, 54, 745-749 https://doi.org/10.2331/suisan.54.745
  16. Seki, S., N. Taniguchi, N. Murakami, A. Takamichi and I. Takahashi. 1994. Seasonal changes in the mixing rate of restocked ayu-juveniles and assessment of native stock using an allozyme marker. Fish. Sci., 60, 31-35 https://doi.org/10.2331/fishsci.60.31
  17. Sugama, K., N. Nobuhiko and S. Umeda. 1988. An experimental study on genetic drift in hatchery population of red sea bream. Nippon Suisan Gakkaishi, 54, 739-744 https://doi.org/10.2331/suisan.54.739
  18. Taniguchi, N., S. Seki and Y. Inada. 1983a. Genetic variability and differentiation of amphidromous, land-locked, and hatchery populations of ayu Plecoglossus altivelis. Nippon Suisan Gakkaishi, 49, 1655-1663 https://doi.org/10.2331/suisan.49.1655
  19. Taniguchi N., K. Sumantadinata and S. Iyama. 1983b. Genetic change in the first and second generations of hatchery stock of black sea bream. Aquaculture, 35, 309-320 https://doi.org/10.1016/0044-8486(83)90103-5
  20. Taniguchi, N. and I. Takahashi. 1989. Tracking ayu seed restocked in the population of Nakagawa river using biochemical genetic markers. Month. Kaiyo, 21, 270-276
  21. Toung, K. and S. Zhang. 1998. Studies on the technique of release and stock transfer for stock enhancement of fleshy prawn in China. Kor. Aquacult., 10, 137-143
  22. Toyoya, K., T. Miyajima, T. Joke, Y. Matsuda and N. Ohtsuki. 1997. Efficiency of uropod cutting for marking young kuruma prawn Penaeus japonicus-II conditions for regeneration. Sabaigiken, 25, 95-100
  23. Toyota, K., T. Miyajima, K. Yoshida, Y. Fujita and T. Sakaiya. 1998. Efficiency ofuropod cutting for marking young kuruma prawn Penaeus japonicus-III effecttive marking size. Saibaigiken, 26, 85-90
  24. Toyota, K., T. Yamauchi and T. Miyashima. 2003. A marking method of cutting uropods using malformed regeneration for kuruma prawn Marsupenaeus japonicus. Fish. Sci., 69, 161-169 https://doi.org/10.1046/j.1444-2906.2003.00601.x

Cited by

  1. Lack of Genetic Differentiation in the Shrimp Penaeus Chinensis in the Northwestern Pacific vol.45, pp.7, 2006, https://doi.org/10.1007/s10528-007-9098-6
  2. Genetic differentiation in seven geographic populations of the fleshy shrimp Penaeus (Fenneropenaeus) chinensis based on microsatellite DNA vol.287, pp.1, 2006, https://doi.org/10.1016/j.aquaculture.2008.10.030