The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Genetic Variation of the Pleurotus ostreatus Complex Based on Isozyme Analysis

동위효소 분석에 의한 Pleurotus ostreatus Complex의 유전적 변이

  • Lee, Hee-Kyung (Chungbuk Agricultural Research and Extension Services) ;
  • Yoo, Young-Bok (Division of Applied Microbiology, National Institute of Agricultural Science and Technology, RDA) ;
  • Min, Kyung-Hee (Department of Biology, Sookmyung Women's University)
  • Published : 1999.10.30
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Abstract

Isozyme comparisons of mycelial extracts from Pleurotus ostreatus were undertaken using isoelectric focusing. Enzyme isozyme patterns were Used to describe the extent of geographical diversity and degree of intraspecific variation in these extracts. A total of 77 bands were resolved from six different enzymes. Cluster analyses were performed using the zymograms for esterase (EST), glucose phosphate isomerase (GPI), leucine aminopeptidase (LAP), malate dehydrogenase(MDH), peroxidase (POX), and phosphoglucomutase (pGM). EST gave multiple banding patterns, while less variability was observed for GPI, MDH, and PGM. Cluster analyses demonstrated that strains of P. ostreatus from geographically different origins are genetically divergent, supporting the idea that there is little or no gene flow between these geographically distant population groups.

Keywords

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