Effects of Aqueous Extracts of Pinus rigida on Protein and Isozyme patterns during Radish Germination

리기다소나무의 수용추출액이 무 종자의 발아과정에서 단백질과 동위효소 패턴에 미치는 영향

  • 김용옥 (건국대학교 이과대학 생물학과) ;
  • 이호준 (건국대학교 이과대학 생물학과)
  • Published : 1998.12.01

Abstract

Aqueous extracts of Pinus rigida changed the electrophoretic patterns of total proteins and of hydrolytic enzymes such as peroxidase, esterase and amylase during the germination of radish (Raphanus sativus var. hortensis for. acanthiformis). When the extract treatment was finished, at the late stage of radish germination, aqueous extracts of P. rigida had suppressed the expression of 24 KD and 60 KD proteins. the extract induced new isozyme bands, indicating concomitant activity of peroxidases, esterase activities were stimulated in the cathodic region. The activity of amylase was enhanced by the extract.

Keywords

References

  1. Theor. Appl. Genet. v.67 Characterization of another differentiation in cytoplasmic male sterile maize using a specific isozymic system (esterase) Abbot, A.G.;C.C. Ainsworth;R.B. Flavell
  2. Agron. J. v.76 Allelopathic effects of annual weed residues of growth and nutrient uptake of corn and soybeans Bhowmik, P.C.;J.D. Doll
  3. Plant Physiol. v.49 Tannins as gibberellin antagonists Corcoran, M.R.;T.A. Geissman;B.O. Phinney
  4. Am. J. bot. v.62 Effects of phenolic inhibitors on growth and metabolism of glucose-UL$-^{14}$C in Pauls Scarlet Rose cell-suspension cultures Danks, M.L.;J.S. Fletcher;E.L. Rice
  5. Oecologia v.9 On the variability of chlorogenic acid concentration Del Moral, R.
  6. Arch. Biochem. Biophys. v.106 Accumulation of ayapin and scopolin in sunflower plants treated with 2,4-dich-lorophenoxy acetic acid Dieterman, L.J.;C.Y. Lin;L.M. Robrbaugh;S.H. Weder
  7. Physiological-Biochemical Basis of Plant Interactions in Phytocenoses v.2 On biochemical interaction of cultivated plants and weeds Dzyubenko, N.N.;N.I. Petrenko;A.M. Grodzinsky(ed.)
  8. Plant Physiol. v.81 immunocytochemical localization and time course of appearance of an anionic peroxidase associated with suberization in wound-healing potato tuber tissue Espelie, L.E.;V.R. Franceschi;P. E. Kolattukudy
  9. Can. J. Plant Sci. v.57 Influence of seed size protein content and cultivar on early seedling vigor in wheat Evans, L.E.;G.M. Bhatt
  10. Physiol. Plant v.64 A two-step control of basic and acidic peroxidase and its significance growth and development Gaspar, T.;C. Penel;F.J. Castillo;H. Greppin
  11. Alleopathy Effect of pine allelochemicals on selected species in Korea Kil, B.S
  12. J. Chem. Ecol. v.9 Allelopathic effects of pinus densiflora on the floristic composition of undergrowth of red pine forest Kil, B.S.;Y.J. Yim
  13. Phytochemistry v.19 Isolation and characterization of two isoperoxidase from tobacco tissue cultures Kim, S.S.;S.H. Wender;E.C. Smith
  14. Fiziol. Rast. (Moscow) v.23 The rate of protein biosynthesis and adsorption of minenal sustances by the roots of oak and ash growing together in the forest Kolesnichen, M.V.;M.M. Aleikina
  15. Physiological-Biochemical Basis of Plant Interactions in Phytocenoses Naukova Dumka, Kiev. Vol.Ⅰ v.1 Influence of potatoes on an apple tree and its photosynthesis Krylov, Y. V.;A.M. Grodzinsky(ed.)
  16. Nature v.227 Cleavage of structural proteins during the assembly of the head of bacteriophage T4 Laemnli, U.K.
  17. Am. J. Bot. v.67 Effects of pine-produced chemicals on selected understory species in a Pinus ponderosa community Lodhi, M.A.K.;K.T. Killngbeck
  18. Phytopathology v.55 Peroxidase activity in virus-infected sweet potatoes Loebenstein, G.;N. Linsey
  19. Pflanzen Physiol. v.82 Mader, M.;A. Nessel;M. Bopp
  20. Vegetatio v.18 Allelopathy as a factor in ecological process Muller, C.H.
  21. Electrophoresis. Isoelectricfocusing. Ultracentrifugation Methods of protein and nucleic acid research.Ⅰ. Osterman, L.A.
  22. Allelopathy(2nd ed.) Rice, E.L.
  23. Plant and Cell Physiol. v.222 Isozymes of glucose-6-phosphate dehydrogenase and $NAD^+$ malate dehydrogenase in shoot forming foliar discs of tobacco Seeni, S.;A. Gnanam
  24. Electrophoresis and focusing in slabs using the Panta-Phorapparatus for analytical and preparative separation in gels Stegman, H.M.;W. Burgermeister;H. Francksen;M. Krogerrecklenfort
  25. Recent Adv. Phytochem v.4 Biochemical studies in relation to the possible germination regulatory role of naturally occurring coumarin and phenolics van Sumee, C.F.;J. Cottenie;J.D. Greef;J. Kint
  26. Worthington Enzyme Manual Worthington Biochemical Corporation
  27. Korea Biochem. J. v.21 Purification and characterization of an anionic isoperoxidase from Korean radish root Yoo, W.I.;S.S. Kim