• Korean journal of applied entomology
• /
• v.46 no.1 s.145
• /
• pp.131-139
• /
• 2007

From the course of screening of useful enzyme producing microorganism from insect guts, we isolated 9 lipase producing strains and their lipase producing activities were tested. 16S rDNA sequence analysis showed that they were Gram negative bacteria grouped on Serratia sp., Pseudomonas sp., and Burkholderia sp.. Among them, an excellent lipase producing strain, Burkholderia sp. HY-10 identified by 16S rDNA analysis and biochemical methods, was further studied its lipase producing characteristics. It was isolated from a longcorm beetle, Prionus insularis and showed cell density dependent lipase producing activity in the culture media that contained olive oil as a carbon source. Maximum lipase production was achieved in the M9 media containing 0.5% yeast extract and 0.5% olive oil when cultured at $30^{\circ}C$ for 36-42 hrs.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.36 no.1
• /
• pp.15-24
• /
• 2018

N-glycosylation is an important posttranslational modification that results in a variety of biological activities, structural stability, and protein-protein interactions. There are still many mysteries in the structure and function of N-glycans, and detailed elucidation is necessary. Baculovirus expression system (BES) is widely used to produce recombinant glycoproteins, but it is not suitable for clinical use due to differences in N-glycan structure between insects and mammals. It is necessary to develop adequate model glycoproteins for analysis to efficiently alter the insect-type N-glycosylation pathway to human type. The previous research shows the recombinant alpha 1-acid glycoprotein (${\alpha}1AGP$) secreted from silkworm cultured cells or larvae is highly glycosylated and expected to be an excellent research candidate for the glycoprotein analysis expressed by BES. Therefore, we improved the ${\alpha}1AGP$ to be a better model for studying glycosylation. The modified ${\alpha}1AGP$ (${\alpha}1AGP{\Delta}$) recombinant protein was successfully expressed and purified by using BES, however, the expression level in silkworm cultured cells and larvae were lower than that of the ${\alpha}1AGP$. Subsequently, we confirmed the detailed profile of N-glycan on the ${\alpha}1AGP{\Delta}$ by LS/MS analysis the N-glycan structure at each glycosylation site. These results indicated that the recombinant ${\alpha}1AGP{\Delta}$ could be usable as a better model glycoprotein of N-glycosylation research in BES.

• Korean journal of applied entomology
• /
• v.57 no.1
• /
• pp.33-38
• /
• 2018

Walnuts are one of the most economically important fruit trees in forestry. In walnut orchards, however, studies examining damage by insect pests are limited. This study was conducted to investigate the rate of damage caused by Conogethes punctiferalis to walnut fruits and by Acrocercops transecta to walnut leaves. In 2017, the change of damage rate (caused by C. punctiferalis) to fruits were investigated in Buyeo. In addition, we counted fruits and leaves damaged by two lepidopteran species in Gimcheon, Buyeo, Yeongdong, and Hwaseong. Damaged fruits steadily increased in abundance with fruit growth in Buyeo, and the highest number of damaged fruits was documented in late summer. The rates of damage caused by C. punctiferalis to fruits in Buyeo, Gimcheon, and Hwaseong were 22.1%, 20.5%, and 11.7%, respectively. Additionally, the rates of damage caused by A. transecta to leaves in seedlings (58.5% on average) were significantly higher than to those in older trees (11.2% on average), irrespective of the study location. In conclusion, this study showed that C. punctiferalis and A. transecta were serious pests affecting walnut fruits and leaves, respectively. Therefore, management methods for these insect pests need to be developed.

• Korean journal of applied entomology
• /
• v.54 no.4
• /
• pp.409-417
• /
• 2015

This study aimed to summarize the characteristics of visitor experience for different market segments based on insect experience orientation in order to develop Ptecticus tenebrifer (Diptera: Stratiomyidae [Walker]) experience programs using local resources in the insect industrial market. A total of 325 effective samples were collected, and the attributes of insect experience orientation were connected to education, emotion, society, and ego. The survey respondents were students and parents of school-age children. Cluster analysis showed that the respondents were classified into two or three groups that were all found to be high in the prediction validity and discriminant analysis. Based on this result, the respondents were properly subdivided into three clusters for final analysis. Those three groups were: the passive group, the moderate group, and the active-orientation group. As a group aiming for various insect experiences, the active-orientation group consisted of parents whose children range in age from either 5 to 9 or from 10 to 14. This group was willing to spend 7 ~ 8,000 won on each experience program, and they preferred emotional experience programs. The passive and moderate orientation groups were most interested in having an educational experience. In order to increase people's interest in experience programs, education-oriented programs should be developed and promotional marketing should be reinforced. The results and implications of this study may be useful for the development of insect experience programs that can meet various visitor experience orientations as basic data that can contribute to the vitalization of the 6th industrialization plan for the insect industry.

• The Korean Journal of Emergency Medical Services
• /
• v.17 no.1
• /
• pp.63-78
• /
• 2013

Purpose : This study aimed to improve quality of prehospital emergency care for the insect bite patients by figuring out its current situations and problems. Methods : This study was conducted to 219 insect bite patients who were transferred to the hospital by 119 ambulance in D-City from July 1, 2009 to June 30, 2011. This study was a descriptive study by 119 run sheets. A total of 171 emergency medical technicians(EMTs) working in D-city and C-province completed the questionnaire. All of data were analyzed by SPSS 18.0. Results : Among the 219 patients, bee sting accounted for 62.6% and wasp sting accounted for 20.1%. Some patients complained of dizziness, headache, dyspnea, and disturbance of consciousness. The 119 EMTs initially treated the patients with oxygen supply to 57 patients (31.1%). Knowledge toward insect bite was statistically significant (p=.000) between advanced EMTs and basic EMTs. The obstacle to the prehospital care, many EMTs answered that the obstacle was due to the lack of 119 EMTs. Conclusion : It is necessary to differentiate the patients' symptoms and provide the better prehospital emergency care to verify the exact symptoms and signs.

• Korean journal of applied entomology
• /
• v.56 no.2
• /
• pp.153-164
• /
• 2017

Gene silencing using double-stranded RNA (dsRNA) has been widely used in functional genomics in biological organisms. Its principle stems from RNA interference (RNAi), a post-transcriptional control of gene expression. Suppression of specific gene expression using dsRNA may give significant lethal effect. Insect pest control exploits this molecular process to develop novel insecticides using specific dsRNAs. This review explains core principles of RNAi using dsRNA. Then it illustrates various examples to control insect pests using dsRNAs. It also discusses limitations to control insect pests using dsRNAs. Finally, it provides several breakthroughs to develop dsRNA insecticides.

• Journal of Microbiology
• /
• v.44 no.1
• /
• pp.77-82
• /
• 2006

The tea looper caterpillar, Ectropis obliqua, is one of the major pests of tea bushes. E. obliqua single-nucleocapsid nucleopolyhedrovirus (EcobSNPV) has been used as a commercial pesticide for biocontrol of this insect. However only limited genetic analysis for this important virus has been done up to now. EcobSNPV was characterized in this study. Electron microscopy analysis of the occlusion body showed polyhedra of 0.7 to $1.7\;{\mu}m$ in diameter containing a single nucleocapsid per envelope of the virion. A 15.5 kb genomic fragment containing EcoRI-L, EcoRI-N and HindIII-F fragments, was sequenced. Analysis of the sequence revealed that the fragment contained eleven potential open reading frames (ORFs): lef-1, egt, 38.7k, rrl, polyhedrin, orfl629, pk-1, hoar and homologues to Spodoptera exigua multicapsid NPV (SeMNPV) ORFs 15, 28, and 29. Gene arrangement and phylogeny analysis suggest that EcobSNPV is closely related to the previously described Group II NPV. Bioassays on lethal concentration $(LC_{50}\;and\;LC_{90})$ and lethal time $(LT_{50}\;and\;LT({90})$ were conducted to test the susceptibility of E. obliqua larvae to the virus.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2004.10a
• /
• pp.826-831
• /
• 2004

Recently, studies have been carried out to develop unmanned Micro Air Vehicles(MAVs) that can search and monitor inside buildings during urban warfare or rescue operations in hazardous environments. However, existing fixed-wing and rotary-wing MAVs cannot travel at extremely low or high speeds, hover in place, or change directions instantly. This has lead researches to search for other flight methods that could overcome those drawbacks. Insect flight principles and its applications to MAVs are being studied as an alternative flight method. To take flight, insects flap and rotate their wings. These wing motions allow for high maneuverability flight such as hovering, vertical take off and landing, and quick acceleration and deceleration. This paper proposes a method for designing a mechanism that reproduces hovering insect flight, the basis for all other forms of insect flight. The design of a mechanism that can reproduce the motion that causes maximum lift is proposed, the required specifications are calculated, and a method for reproducing hovering insect flight with a single motor is presented. Also, feasibility of the design was confirmed by simulation.

• International Journal of Industrial Entomology and Biomaterials
• /
• v.31 no.2
• /
• pp.90-94
• /
• 2015

Several insects have been shown to exert a strong influence positively on peoples' emotions. Insect-mediated mental healthcare program was designed to help meet the physical, behavioral and developmental needs of adolescents. They were provided with an insectmediated mental healthcare program for a total of 10 sessions, one session per wk, at 1 h per session, accompanied by a pre- and post-test for emotion. Significant differences and correlations were found according to the individual participants' planning, subjective quality of life, self-reported depression and self-worth, respectively. The results indicated that adolescents who participated in the insect-mediated healthcare program group once showed significant improvement in their planning, subjective quality of life and self-worth. However, most of all participants' self-reported depression was not influenced. These findings suggest that pet-insects positively affect adolescent's emotional health via an insect-mediated healthcare program.

• Asian Journal of Turfgrass Science
• /
• v.24 no.2
• /
• pp.182-190
• /
• 2010

This study was conducted to know the insect fauna on the 5 green Parks areas of Daegu Metropolitan city. Materials were collected by net sweeping and pitfall trap of cup. As the result, 100 species of 93 genera belonging to 61 families in 9 orders were surveyed, among them, Yeonam Park showed highest insect occurrence as 61 species belonging to 35 families of 8 orders. The other side, lowest site was Gukchae-Bosang memorial Park as 2 species from 2 orders. There was more species in broad-leaf tree area than mixture area of broadleaf and needle-leaf trees. Turf grass areas in the Parks showed low insect diversities.