• Korean Journal of Soil Science and Fertilizer
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• v.22 no.1
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• pp.61-66
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• 1989

Plants were exposed to different sub-optimal growth temperature regimes for hardening or sprayed with abscisic acid (ABA) before being placed at $10^{\circ}C$ for three days. Comparisons were made with non-treated plants for plant survival, photosynthetic activities and fatty acids composition of phospholipids. The level of endogenous ABA of the hardened seedlings was compared with that in control and ABA sprayed-seedlings. The results of the above experiment were summarized as follows: 1. Compared to non-treated plants, the hardening and ABA treatment resulted in higher plant survival rate and better seedling growth following earlier transplanting. The low temperature hardening which gradually increased the duration of the low temperature treatment at 2-day intervals was more effective than the controled temperature hardening which gradually decreased the temperature from $25^{\circ}C$ to $20^{\circ}C$ and $15^{\circ}C$. 2. The photosynthetic activities of rice leaves after 3-day exposure to $10^{\circ}C$ were higher in hardened and ABA sprayed seedlings than in non-treated plants. The root activities after low temperature treatment were also higher in hardened and ABA sprayed seedlings than in control. 3. Fatty acids of phospholipids in rice plants such as stearic acid and oleic acid were decreased and the highly unsaturated fatty acids, linoleic acid and lenolenic acid were increased by hardening or ABA treatments. It is assumed that the increase of cold tolerance in these plants was due to the higher ratio of unsaturated to saturated fatty acids. Respectively, the ratio was 1.86, 1.97 and 1.80 in plants given controled temperature hardening, the low temperature hardening and ABA treatments. While in control plants, it was 1.17. 4. ABA content in rice seedling given the low temperature hardening treatment was 67.2 ng/g F.W which was approximately twice that of non-treated control, which was 33.9 ng/f F. W., in contrast, the ABA content of the ABA treated seedlings was 91.7 ng/g F. W.

• Korean Journal of Food Science and Technology
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• v.34 no.2
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• pp.255-262
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• 2002

This experiment was performed to improve the stability of Lactobacillus fermentum YL-3 as a poultry probiotics. The culture conditions that improve acid tolerance of L. fermentum YL-3 were investigated by changing several factors such as medium composition, temperature, anaerobic incubation and culture time. Also, L. fermentum YL-3 was encapsulated with alginate, calcium chloride and chitosan. The stable culture conditions of L. fermentum YL-3 were obtained in anaerobic incubation using MRS media without tween 80 for 20 hour at $42^{\circ}C$. The capsule after treatment with 1% chitosan was formed close membrane by a bridge bond. Immobilization of L. fermentum YL-3 in capsule was observed by confocal laser scanning microscopy, and cell viability was $2.0{\times}10^9\;CFU/g$ above the average. L. fermentum YL-3 capsule after acid treated at pH 2.0 for 3 hour survived about 40%, but those encapsulated with 1% chitosan survived about 65%. Survival rate of capsule stored at room temperature decreased about $2{\sim}3$ log cycle during 3 weeks, but viability of capsule stored at $4^{\circ}C$ during 3 weeks maintained almost $10^8\;CFU/g$ levels.

• Herbal Formula Science
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• v.31 no.1
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• pp.1-10
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• 2023

Objectives : The main aim of this study was to examine the LC-MS/MS used to identify phenolic compounds of CRE(Coptidis Rhizoma 70% EtOH Extract). Also, we investigated antioxidative activities and Anti-inflammatory activities. Methods : LC-MS/MS Analysis HPLC and LC-MS/MS were performed on a 1260 series HPLC system (Agilent Technologies, Inc., California, USA) and 3200 QTrap tandem mass system (Sciex LLC) operated in positive ion mode (spray voltage set at -4.5 kV). The solvent used was DW and Acetonitrile containing 0.1% formic acid, a gradient system was used at a flow rate of 0.5 mL/min for analysis, and a Prontosil C18 column (length, 250 mm; inner diameter, 4.6 mm; particle size, 5 ㎛; Phenomenex Co., Ltd., California, USA, Biochoff Chromatography) was used. The solvent conditions used in the mobile phases were 0-10 min at 10-15% B, 10-20 min at 20% B, 20-30 min at 25%, 30-40 min at 40%, 40-50 min at 70%, 50-60 min at 95%, and 60-70 min at 95%. The analysis was performed at a wavelength of 284 nm and a temperature of 35℃. The cell viability was measured using a 3-(4,5-dimethyethiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity. We examined the effects of CRE on the lipopolysaccharide (LPS)-induced production of nitric oxide (NO) in a RAW 264.7 cells Results : The chemical analysis CRE by Liquid chromatography-tandem mass spectrometry (LC-MS/MS) confirmed that Rosmarinic acid, Ferrulic acid, 3-O-feruloylquinic acid, and 5-O-feruloylquinic acid as phenolic components. DPPH radical scavenging activity was the inhibitory activity of CRE showed at 200 ㎍/mL a statistically significant level. MTT assay demonstrated that the CRE did not have a cytotoxic effect in RAW 264.7 and LPS-induced RAW264.7 cells. Also, CRE reduced NO production in RAW 264.7 cells stimulated with LPS. Conclusions : Based on these findings, The chemical analysis 4 major components CRE such as Rosmarinic acid, Ferrulic acid, 3-O-feruloylquinic acid, and 5-O-feruloylquinic acid. Moreover, we confirmed that CRE has effects antioxidant and anti-inflammatory. The results demonstrate that CRE can be used as an antioxidant and a powerful chemopreventive ingredient against inflammatory diseases.

• Journal of the Korean Society of Fisheries and Ocean Technology
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• v.40 no.4
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• pp.268-281
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• 2004

A study was carried out in order to estimate the deformation of the pound net according to the current by the model test in the circulating water channel. The tension of the frame rope and the variation of net shape were measured to investigate the deforming of the model pound net in the flow. The results are obtained as follows; 1. The experimental equation between tensions (R) of the frame rope and velocity (ν)was found to be R=$19.58v^{1.98}$($r^2$=0.98) in case of the upperward flow with fish court net and R=$26.90v^{1.72}$($r^2$=0.95)at the upperward flow with bag net according to the velocity from 0.0m/s to 0.6m/s, respectively. 2. As the variation of flow speed inside of the model net was gradually decreased according as which is passed through netting panels, in case of the upperward flow with fish court net, the flow speed was about 70% of initial flow speed at 0.1m/s, 60% at 0.2m/s, 50% at 0.3m/s and 40% 0.4~0.6m/s at the measurement point(h) inside of the first bag net, respectively. In case of the upperward flow with bag net, as the flow speed was steeply decreased according as which if passed through the second bag net, it was 30~60% of the initial flow speed and was 20~30% inside of the first bag net and was about 10~20% inside of the inclined passage net. 3. In case of the upperward flow with fish court net, the variation of deformed angle of fish court net was from 0$^{\circ}$ to 70$^{\circ}$and that of inclined passage net was from 0$^{\circ}$ to 63$^{\circ}$and that of the second bag net was from 0$^{\circ}$ to 47$^{\circ}$ . 4. In case of the upperward flow with fish court net, the variation of deformed angle of the second bag net was changed from 0$^{\circ}$ to 70$^{\circ}$and that of the inclined passage net was from 0$^{\circ}$ to 55$^{\circ}$ and that of the fish court net was from 0$^{\circ}$ to 50$^{\circ}$. The depth ratio of the first bag net was changed from 0% to 35% and that of the second bag net was from 0% to 20% and that of the inclined passage net was from 0% to 35%. In the flow speed 0.5m/s, the inclined passage net was raised up to the entry of the bag net and then prevented it more over 90%. 5. To be increased the opening volume of pound net, it needs to attach the added weight outside of the fish court net, inclined passage net and bag net. At the same time, it needs to adjust the tension of the twine for maintenance of the shape.

• Radiation Oncology Journal
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• v.19 no.2
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• pp.87-94
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• 2001

Purpose : To evaluate the role of LINAC-based stereotactic radiosurgery (SRS) in the management of meningiomas, we reviewed clinical response, image response, neurological deficits for patients treated at our institution. Methods and materials : Between February 1995 and December 1999, twenty-six patients were treated with SRS. Seven patients had undergone prior resection. Nineteen patients received SRS as the initial treatment. There were 7 male and 19 female patients. The median age was 51 years (range, $14\~67\;years$). At least one clinical symptom presented at the time of SRS in 17 patients and cranial neuropathy was seen in 7 patients. The median tumor volume was $4.7\;cm^3\;(range,\;0.7\~16.5\;m^3)$. The mean marginal dose was 15 Gy (range, $10\~20\;Gy$), delivered to the $80\%$ isodose surface (range, $46\~90\%$). The median clinical and imaging follow-up periods were 27 months (range, 1-71 months) and 25 months (range, $1\~52\;months$), respectively. Results : Of 14 patients who had clinical follow-up of one year or longer, thirteen patients $(93\%)$ were improved clinically at follow-up examination. Clinical symptom worsened in one patient at 4 months after SRS as a result of intratumoral edema, who underwent surgical resection at 7 months. OF 14 patients who had radiologic follow-up of one year or longer, tumor volume decreased in 7 patients $(50\%)$ at a median of 11 months (range, $6\~25\;months$), remained stable in 6 patients $(43\%)$, and increased in one patient $(7\%)$, who underwent surgical resection at 44 months. New radiation-induced neurological deficits developed in six patients $(23\%)$. Five patients $(19\%)$ had transient neurological deficits, completely resolved by conservative treatment including steroid therapy. Radiation-induced brain necrosis developed in one patient $(3.8\%)$ at 9 months after SRS who followed by surgical resection of tumor and necrotic tissue. Conclusions : LINAC-based SRS proves to be an effective and safe management strategy for small to moderate sized meningiomas, inoperable, residual, and recurrent, but long-term follow-up will be necessary to fully evaluate its efficacy. To reduce the radiation-induced neurological deficit for large size meningioma and/or in the proximity of critical and neural structure, more delicate treatment planning and optimal decision of radiation dose will be necessary.

• Journal of Ginseng Research
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• v.31 no.4
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• pp.196-202
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• 2007

Skin wrinkles are associated with collagen synthesis and matrix metalloproteinase-I (MMP-1) activity. This study was carried out to select optimum ratio of 3 herbs in skin health food for anti-wrinkle. Human dermal fibroblast cell was incubated with experimental samples, which were Korean red ginseng ethanol extracts (ER), Torilis fructus water extracts (WT), Corni fructus water extracts (WC) and their mixtures (WM1, WM3). And then we determined effects on collagen biosynthesis, MMP-1 activity and SOD activity in human dermal fibroblast cell. In control group, collagen biosynthesis was amounted at 474.8 ng/ml and 533.9 ng/ml, 539.3 ng/ml, 514.1 ng/ml in ER, WT and WC respectively. Furthermore, WM3 (KTNG0345) was increased to 561.45 ng/ml. MMP-1 activity of ER, WT, WC, WM1 were determined to 31.9 ng/ml, 32.85 ng/ml, 32.0 ng/ml, 31.3 ng/ml and WM3 (KTNG0345) was decreased to 28.85 ng/ml. In addition, the experimental samples showed a antioxidative activities. From this results, we conclude that Korean red ginseng ethanol extracts, Torilis fructus water extracts, Corni fructus water extracts and their mixtures have a anti-wrinkle effect and WM3 (KTNG0345) may be regarded as an optimum composition for synergic effect producing. The standardized components of KTNG0345, ginsenoside-$Rb_1$, torilin and loganin were identified at 10.85 mg/g, 0.128 mg/g and 3.92 mg/g respectively.

• Korean Journal of Plant Resources
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• v.27 no.1
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• pp.1-10
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• 2014

Essential oils of various plants have been known for potential biological effects such as antibacterial, antifungal, spasmolytic, antiplasmodial activities and insect-repellent property. Recently, the essential oils have attracted considerable interest in oral disease therapy. This essential oil has been known as being effective on easing sick house syndrome, giving forest aroma therapy effect and acting as repellent against pest. The essential oil of Pinus koraiensi, a native plant from Hongcheon-gun, Gangwon-do, was obtained by hydrodistillation. In light of its medicinal importance, in this study its composition, antibacterial activity and the reducing effect of offensive odor have been analyzed. The composition of essential oil was determined by GC and GC-MS. We have identified 14 compounds, of which 1R-${\alpha}$-pinene (19.38 %), 3-carene (10.21 %), camphene (9.82 %), limonene (9.00 %), bicyclo[2,2,1] heptan-2-ol (8.76 %) and ${\beta}$-phellandrene (7.98 %) were the main components. Essential oils from P. koraiensis, Chamaecyparis obtusa, Abies holophylla and Pinus densiflora were compared in terms of alleviating effect of malodors caused from formaldehyde, ammonia, trimethylamine and methylmercaptan. P. koraiensis essential oil was found to decrease the amounts of ammonia and trimethylamine by 75.17 % and 77.36 %, respectively. Antibacterial activity against Streptococcus mutans and Streptococcus sobrinus, which were known as oral cavity inducer, was investigated using the paper disc agar diffusion method. The inhibition zone was observed against S. mutans (5.97 mm) and S. sobrinus (1.40 mm), respectively. P. koraiensis essential oil shown effective deodorization and inhibitory activity against oral cavity in this study might be potential material in oral sanitary industry.

• Journal of The Korean Society of Grassland and Forage Science
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• v.38 no.4
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• pp.253-259
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• 2018

A new barley (Hordeum vulgare L.) cultivar 'Mihan' having ruminant-palatable semi-smooth awn and good silage quality was developed at National Institute of Crop Science(NICS), RDA in 2016. This cultivar was derived from a cross of the 'SB00T2064' and 'Suwon385' in 2001. And its promising line showed high yielding through the preliminary yield trials(PYT) and advanced yield trials (AYT) in Iksan from 2012 to 2013. It was designated as the 'Iksan487'. 'Iksan487' was conducted to regional yield trials (RYT) in one upland field and five paddy fields around Korea for three years from 2014 to 2016. And it was released as the name of 'Mihan'. It has growth habit of IV, erect plant type, green leaf and semi-smooth awn. Its heading date was April 23 in the paddy field and maturing date was May 24. Plant height of 'Mihan' was 96cm. Mihan's spikes per $m^2$ was 665. It has better winter hardiness and resistance to BaYMV (Barley Yellow Mosaic Virus) than that of barley cultivar 'Youngyang'. The average dry matter of 'Mihan' was about $10.9ton\;ha^{-1}$ in paddy field. And average feed quality of 'Mihan' was 10.3% of crude protein content, 26.1% of ADF (Acid Detergent Fiber), 46.9 % of NDF (Neutral Detergent Fiber) and 68.2% of TDN (Total Digestible Nutrients). 'Mihan' had grade I of silage quality. This cultivar would be suitable for the area above the daily minimum average temperature of $-8^{\circ}C$ in January in Korean peninsula.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.67 no.3
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• pp.180-188
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• 2022

This study was conducted to determine the optimal sowing date for seed yield of summer oat (Avena sativa L.) cultivars "Darkhorse (DH)" and "Highspeed (HS)" in Wanju, Jeonbuk province between 2017 and 2018. We investigated seed yield from 4 sowing dates: July 15, July 30, August 15, and August 30. We evaluated the agronomic characteristics of summer oats (DH and HS). We found the heading date of all cultivars to be within 50 days. Delayed sowing resulted in significantly increased plant height for both years and cultivars. There was no significant difference in spike length of DH and HS which ranged from 12.8 to 17.8 cm. The sowing date of July 30 produced a higher number of grains per spike, but this yield differed significantly by year and cultivars. In 2017, the first sowing resulted in the lowest DH yield at 132 kg per 10a, while the second sowing had the highest yield at 227 kg. HS yield was the lowest in the first sowing at 126 kg and the highest in the third sowing at 219 kg. In 2018, DH had the lowest yield from the first sowing at 184 kg per 10a, and the highest from the second sowing at 240 kg, but there was no significant difference between these yields. The first sowing for HS gave the lowest yield at 160 kg, and the second sowing produced the highest at 258 kg. The germination rate of harvested seeds from each sowing date in 2017 and 2018 was found to be higher than 85% and there was no significant difference between the two cultivars in the 2018 germination rate test. Thus, we found the optimal sowing date for summer cultivation of oats for the highest seed yield to be between July 30 (second sowing) and August 15 (third sowing).

• Journal of Acupuncture Research
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• v.22 no.3
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• pp.137-144
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• 2005

Objective : This study was designed to assess the heart rate variablitv of patients with Parkinson's disease according to Hoehn-Yahr stage. Methods : Subjects were voluntarily recruited by newspapers and internet. All the subjects are confirmed as idiopathic parkinson's disease by a neurologist. The patients were grouped by Hoehn-Yahr(H-Y) stage. Heart rate variability was measured for 5 minutes after 5 minutes' bed rest. Subjects were comprised of 3 groups which were H-Y stage I (n=14), H-Y stage H(n=9), H-Y stage III(n=7). We compared the heart rate variability of each groups by one-way ANOVA test. We also studied the relationship between UPDRS score and Heat rate variability components (SDNN, RMS-SD and TP) by pearson correlation analysis and simple linear regressin analysis Results: Age, duration was matched among groups. SDNN, RMS-SD, TP were significantly decreased as H-Y stage increased(p<0.05). Especially there was significant difference between H-Y stage I and III group(p<0.05). But other components(Heart rate, LF, HF, LF/HF ratio) were not showed significant difference. SDNN, RMS-SD and TP were significantly decreased as UPDRS scores increase(p<0.05). Conclusion : This study suggests that the function of the autonomic nervous system decreases as Parkinson`s disease progresses.