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http://dx.doi.org/10.5142/JGR.2007.31.4.196

Effects of Korean red ginseng and herb extracts mixture (KTNG0345) on procollagen biosynthesis and matrix metalloproteinase-1 activity in human dermal fibroblast  

So, Seung-Ho (Health Food Research Group, KT&G Central Research Institute)
Lee, Seong-Kye (Health Food Research Group, KT&G Central Research Institute)
Hwang, Eui-Il (Health Food Research Group, KT&G Central Research Institute)
Koo, Bon-Suk (Health Food Research Group, KT&G Central Research Institute)
Han, Gyeong-Ho (Health Food Research Group, KT&G Central Research Institute)
Kim, Na-Mi (Health Food Research Group, KT&G Central Research Institute)
Publication Information
Journal of Ginseng Research / v.31, no.4, 2007 , pp. 196-202 More about this Journal
Abstract
Skin wrinkles are associated with collagen synthesis and matrix metalloproteinase-I (MMP-1) activity. This study was carried out to select optimum ratio of 3 herbs in skin health food for anti-wrinkle. Human dermal fibroblast cell was incubated with experimental samples, which were Korean red ginseng ethanol extracts (ER), Torilis fructus water extracts (WT), Corni fructus water extracts (WC) and their mixtures (WM1, WM3). And then we determined effects on collagen biosynthesis, MMP-1 activity and SOD activity in human dermal fibroblast cell. In control group, collagen biosynthesis was amounted at 474.8 ng/ml and 533.9 ng/ml, 539.3 ng/ml, 514.1 ng/ml in ER, WT and WC respectively. Furthermore, WM3 (KTNG0345) was increased to 561.45 ng/ml. MMP-1 activity of ER, WT, WC, WM1 were determined to 31.9 ng/ml, 32.85 ng/ml, 32.0 ng/ml, 31.3 ng/ml and WM3 (KTNG0345) was decreased to 28.85 ng/ml. In addition, the experimental samples showed a antioxidative activities. From this results, we conclude that Korean red ginseng ethanol extracts, Torilis fructus water extracts, Corni fructus water extracts and their mixtures have a anti-wrinkle effect and WM3 (KTNG0345) may be regarded as an optimum composition for synergic effect producing. The standardized components of KTNG0345, ginsenoside-$Rb_1$, torilin and loganin were identified at 10.85 mg/g, 0.128 mg/g and 3.92 mg/g respectively.
Keywords
human dermal fibroblast; collagen biosynthesis; matrix metalloproteinase-I (MMP-1); SOD activity; Korean red ginseng;
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1 Guilian, T., Zhang, T., Yang. F. and Ito, Y. : Separation of gallic acid from Cornus Officinalis Sieb. et Zucc by high speed counter-current chromatography. J. Chromatogr. A. 886, 309-312 (2000)   DOI   ScienceOn
2 Lee, E.B., Choi, B.C. and Cho, T.S. : Pharmacological studies on ether fraction of Corni Fructus. Yakhak Hoeji. 29, 1- 10 (1985)   과학기술학회마을
3 Shin, J.H. banghakhappyenhaesel. p.542, sengbosa, Seoul, Korea (2002)
4 Jeng, B.S. and Shin, M.K. : Dohaehyanyaksajeon. p.416, Yeonglimsa, Seoul, Korea (1990)
5 Choi, W.Y., Chun, H.J., Lee, J.H. and Seung, H.B. : Effects of methanol extract from Cornis fructus on melanogenesis, Korean. J. Pharmacogn. 34, 70-74 (2003)   과학기술학회마을
6 Council of Europea : European Pharmacopoeia. 4th Ed. Ginseng. p.1244-1245, Council of Europea, Paris, France (2001)
7 Kim, Y.J. : Preparation of standard material of medicinal herb(II), p.62-65, KFDA, Seoul, Korea (2002)
8 Loosdrecht, A.A., Nennie, E., Ossenkoppele, G.J., Beelen, R.H. and Langenhuijsen M.M. : Cell mediated cytotoxicity against U937 cells by human monocytes and macrophages in a modified colorimetric MTT assay. A methodological study. J. Immunol Method, 141, 15-22 (1991)   DOI   ScienceOn
9 Taubman, M.B., Goldberg, B. and Sherr, C. : Radioimmunoassay for human procollagen. Science, 186, 11150-11157 (1974)
10 Gross, B. and Lapiere, C. : Collagenolytic activity in amphibian tissue a tissue culture assay. Proc. Natl. Acad. Sci. USA, 54, 1197-1204 (1962)
11 Ukeda, H., Maeda, S., Ishii, T. and Sawamura M. : Spectrophotometric assay for superoxide dismutase based on tetrazolium salt 3'-1- (phenylamino)-carbonyl-3,4-tetrazolium]- bis(4-methoxy-6-nitro) benzenesulfonic acid hydrate reduction by xanthine-xanthine oxidase. Anal Biochem. 5, 206 (1997)
12 Parfitt, A.M., Simon, L.S., Villanueva, A.R. and Krane, S.M. : Procollagen type I carboxy-terminal extension peptide in serum as a marker of collagen biosynthesis in bone. Correlation with iliac bone formation Rates and comparison with total alkaline phosphatase. J. Bone Miner Res. 2, 427- 436 (1987)   DOI   ScienceOn
13 Talwar, H. S., Griffith CGM and Fisher, G. J. : Reduced type I type III procollagens in photodamaged adult human skin. J. Inves. Dermatol. 105, 285-290 (1995)   DOI   ScienceOn
14 Nagase, H. and Woessner, J.F. : Matrix metalloproteinases. J. Biol. Chem. 274, 21491-21494 (1999)   DOI
15 Choi, S. : Epidermis proliferative effect of the Panax ginseng ginsenoside Rb2. Arch. Pharm. Res. 25, 71-76 (2002)   DOI   ScienceOn
16 KFDA : Sikpoomgongeon. pp.29, KFDA, Seoul, Korea. (2002)
17 Kim, B.H., Park, K.W., Kim, J.Y., Jeong, I.Y., Yang, G.H., Cho, Y.S., Yee, S.T. and Seo K.I. : Purification and characterization of anticarciongenic compound from Corni fructus. Korean J. Food Sci. 36, 1001-1007 (2004)   과학기술학회마을
18 Tanno, O., Ota, Y., Kitamura, N., Katsube, T. and Inoue, S. : Nicotinamide increases biosynthesis of ceramides as well as other stratum corneum lipids to improve the epidermal permeability barrier. Br. J. Derm. 143, 524-531 (2000)   DOI   ScienceOn
19 Matsumura, Y. and Ananthaswamy, H. N. : Toxic effects of ultraviolet radiation on the skin. Toxicol. Appl. Pharmacol. 195, 298-308 (2004)   DOI   ScienceOn
20 Miller, C.C., Tang, W., Ziboh, V.A. and Fletcher, M.P. : Dietary supplementation with ethyl ester concentrates of fish oil(n-3) and borage oil(n-6) poly unsaturated fatty acid induces epidermal generation of local putative anti-inflammatory metabolites. J. Invest. Dermatol. 96, 98-103 (1991)   DOI   ScienceOn
21 Lee. E.H., Cho, S.Y., Kim, S.J., Shin, E.S., Chang, H.K. and Lee, T.R. : Ginsenoside F1 protects human HaCaT keratinocytes from ultraviolet-B induced apoptosis by maintaining constant levels of Bcl-2. J. Invest. Dermatol. 121, 607-613 (2003)   DOI   ScienceOn
22 Kim, N.M., Koo, B.S,, Lee, S.K., Hwang, E.L., So, S.H. and Do, J.H. : Effect of Korean red ginseng on collagen biosynthesis and MMP-1 activity in human dermal fibroblast. J. Ginseng Res. 31, 86-92 (2007)   과학기술학회마을   DOI
23 Boelsma, E. and Hendriks. H. Roza. : Nutrition skin care : health effects of micronutrients and fatty acids. Am. J. Clin. Nutr. 73, 853-864 (2001)   DOI
24 Chung, S., Kong. S., Seong, K. and Cho, Y.H. :$\gamma$-Linolenic acid in borage oil reverses epidermal hyperproliferation in guinea pigs. J. Nutr. 132, 3090-3097 (2002)   DOI
25 Yamahara, J., Mibu, H., Sawada, T., Fujimura, H. and Takino, S. : Antidiabetic principles of Corni fructus experimental diabetes induced by streptozotocin. Yakugaku Zasshi 101, 86-90 (1981)   DOI
26 Kang, S., Cho, S., Chung, J.H., Hammerberg, C., Fisher, G.J and Voorhees, J.J. : Inflammation and extracellular Matrix Degradation Mediated by activated transcription factors nuclear factor-kB and activator protein-l in Inflammatory acne lesions in vivo. Am. J. Pathol. 166, 1691-1699 (2005)   DOI   ScienceOn
27 Kim, S.J., Kang, B.Y., Cho, S.Y., Sung, D.S., Chang, H.K., Yeom, M.H., Kim, D.H., Sim, Y.C. and Lee, Y.S. : Compound K induces expression of hyaluronan synthetase 2 gene in transformed human keratinocytes and increases hyaluronan in hairless mouse skin. Biochem. Biophys. Res. Commun. 316, 348-355 (2004)   DOI   ScienceOn
28 Lee, J.H., Lee, B.S., Yang, M.S., Byun, B.S., Kim, W.G., Kim, B.H. and Lee, S.J. : Prevention of Photoaging and Wrinkle Formation in Hairless Mice Dorsal Skin by AP3-03. Korean J. Food Sci. Technol. 37, 989-996 (2005)
29 Namjo, K.J. : Yakyounginsam 2000. pp.268-271, Gonglipchulpan co. Tokyo, Japan (2000)
30 Lee, H.J., Kim, S.R., Kin, J.S., Moon, C.J., Bae, C.S., Jang, J.S., Jo, S.K. and Kim, S.H. : The Effect of Red Ginseng on Ultraviolet B-induced Skin Damages in Mouse. J. Ginseng Res. 30, 194-198 (2006)   과학기술학회마을   DOI
31 Lee, H.J., Kim, S.R., Kim, J.S., Moon, C.J., Kim, J.C., Bae, C.S., Jang, J.S., Jo, S.K. and Kim, SH. : The Effect of Red Ginseng on Ultraviolet B-induced Skin Damages in Mouse. J. Ginseng Res. 30, 194-198 (2006)   과학기술학회마을   DOI
32 Lee, E.B., Kim, S.H. and Kim, T.H. : Anti-inflammatory activities of Torilis japonica fruit. Kor. J. Pharm. 29, 384-390 (1998)
33 Itokawa, H., Minashi, S., Watanabe, K., Natsumoto, H., and Hamanaka, T. : Studies on the constituents of crude drugs having inhibitory activity against contraction of the ileum caused by histamine of barium chloride. Shoyakugaku Zasshi 37, 223-228 (1983)
34 Kim, M.S., Baek, J.H., Park, M.T., Sohn, T.K., Kim, S.E., Lee, J.J. and Kim. KW. : Anti-invasive activity of torilin, a sesquiterpene compound isolated from Torilis japonica. Oncol. Rep. 8, 459-461 (2003)
35 Kitajima, J., Suzuki, N. and Tanaka, Y. : Guaian-type sesquiterpenoid glycosides from Torilis japonica. Chem. Pharm.Bull. 46, 1743-1741 (1998)   DOI   ScienceOn
36 Fujita, S. : Miscellaneous contributions to the essential oils of plant from various territories. II. On the components of essential oils of Torilis japonica (Houtt.) Yakugaku Zasshi. 110, 771-775 (1990)   DOI
37 Kitajima, J., Suzuk,i N., Satoh, M. and Watanabe M. : Sesquiterpenoids of Torilis japonica fruit. 59, 811-815 (2002)
38 Park, J.H. : Sangyongyakyongsikmooldogam, pp.196, Shinilsangsa, Seoul, Korea (2000)
39 Kim, Y.H. : Isolation of constituents from the fruits of Cornus officinalis Siebold. 14, 287-292 (2000)