• Journal of fish pathology
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• v.26 no.3
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• pp.241-254
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• 2013

In order to establish bio-marker systems for the screening of endocrine-disrupting chemicals contaminated in various environment, Vitellogenin(Vtg) bio-marker have been developed to detect Scorpion fish's(Sebastiscus marmoratus) Vtg. Vtg has been induced by administration of estradiol into S. marmoratus, and purified by gel filtration and ion-exchange chromatography from serum of the fish. After immunization of the purified Vtg into BALB/c mouse, hybridomas secreting anti-Vtg antibodies have been produced. The size of induced Vtg in the serum was about 440 kDa by gel filtration using Sepharose CL-6B. By SDS-PAGE analysis, the main band of Vtg, however, was at 175 kDa, and several minor bands have been detected with the main band. Eight different monoclonal antibodies have been produced from established hybridomas and the antibodies did not cross-react with sera from different species of fishes tested in this study except with that of Sebastes hubbsi. These results suggested that the monoclonal antibody of S28 and S15 can used as capture and tracer antibodies for ELISA and ICG assays. The detection systems developed in this study can be used as Bio-marker assays to check endocrine disrupting activity of various chemicals as well as to detect known endocrine disrupting chemicals contaminated in environment.

• 한국노년학
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• v.39 no.1
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• pp.21-35
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• 2019

The purpose of this study was to empirically verify the effects of spousal bereavement and complicated grief level on death anxiety of the elderly. The sample consisted of 1,998 adults who were aged 65 or older. Dependent variable was measured with the Death Anxiety Scale-Korean version (DAS-K). Independent variable was measured with both spousal bereavement and the Inventory of Complicated Grief-Korean version (ICG-K). Multiple regression analysis was performed using SPSS 23.0, adjusting for demographics, psycho-social and health variables. The results indicated that death anxiety level was lower among the bereaved with normal grief (p<.01) than non-bereaved. In contrast, death anxiety level was higher among the bereaved with complicated grief than non-bereaved (p<.01). The study result suggests that the most risky factor for death anxiety is complicated grief rather than the bereavement. Although the bereavement can be a universal experience, the severity and duration of symptoms after the bereavement may not be general. The unhealed emotional and physical pain after the bereavement stimulates death anxiety, and senior citizens who suffer from complicated grief often fail to integrate the bereavement and loss into reality, therefore, may not accept the death phenomenon itself. Anxiety and fear of death can emerge when they cannot acknowledge the bereavement. To manage complex sorrows and mitigate death anxiety, intervention programs should be provided to increase adaptability to the bereavement.

• International Journal of Industrial Entomology and Biomaterials
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• v.18 no.1
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• pp.33-40
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• 2009

A vitellogenin cDNA was cloned from the bumblebee Bombus ignitus. The cDNA encoding B. ignitus vitellogenin (BiVg) is 5473 bases long with an open reading frame of 1773 amino acid residues. BiVg possesses two consensus (RXXR/S) cleavage sites and has the conserved DGXR and GL/ICG motif near its C-terminus. The deduced amino acid sequence of BiVg cDNA showed significant similarity with hymenopteran Vgs (51% identity to Apis mellifera Vg, and $33{\sim}36%$ to other insect Vgs). The BiVg cDNA was expressed as a 200-kDa polypeptide in baculovirus-infected insect Sf9 cells. Northern and Western blot analyses showed the expression of BiVg in fat bodies of pupae and adults. In queens, the expression of BiVg was first detected in late pupal stage fat bodies, and secreted BiVg was also observed in late pupal stage hemolymph.

• Journal of the Korean Data and Information Science Society
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• v.9 no.2
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• pp.237-246
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• 1998

We put the pseudo-random number generator into catagories like MiCG, MuCG, URG, ICG, EICG, and test uniformity and independency by 10,000 times through n empirical trial after selecting this random number generator. Here, from a fraction of data(20, 40, 60, 80, 100) with a significance level of 0.1, 0.05 and 0.01, we drive cumulative frequency with K-S, $X^{2}$, poker, run, autocorrelation test. As a result from the uniformity and independency among five random number generators based on all these data, all random number generator except EICG passed uniformity and independency test, and the URG turn out to be excellent in periodicity.

• Fire Science and Engineering
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• v.19 no.2 s.58
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• pp.69-74
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• 2005

A study of the utilization of inert gas generator(IGG) that generates a large amount of inert gas for the fire extinguishing equipment was performed. In this study, the fire extinction performance of gas generated from a 1GG which is implemented to a gas turbine was investigated. A simulated mixture gas of which composition is the same as that of the gas generated from ICG was used in all experiments. First, the extinction concentration was measured using a cup burner method. The extinction design concentration and the volume of extinction space was calculated by applying the obtained extinction concentration to a method recommended by NFPA 2001. In practical respects, the fire extinction performance of IGG-generated gas was confirmed through the fire suppression experiment within a $2m\times2m\times2m$ room.

• The Transactions of The Korean Institute of Electrical Engineers
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• v.57 no.10
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• pp.1822-1828
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• 2008

This paper presents design parameter calculation methodology and its realization to construction for the 10/350${\mu}s$ lightning impulse current generator(ICG) modelled as double exponential function waveform with characteristic parameters ${\alpha},{\beta}$. Matlab internal function, "fzero" was applied to find ${\lambda}={\alpha}/{\beta}$ which is solution of nonlinear equation linearly related with two wave parameter $T_1$ and $T_2$. The calculation results for 10/350${\mu}s$ lightning impulse current show very good accuracy with error less 0.03%. Two type of 10/350${\mu}s$ ICGs based on the calculated design circuit parameters were fabricated by considering the load variation. One is applicable to the MOV based Surge protective device(SPD) for less 15 kA and the other is to test small resistive devices such as spark gap arrester and bonding device with maximum current capability 30 kA. The tested waveforms show error within 10% in comparison with the designed estimation and the waveform tolerance recommended in the IEC 61643-1 and IEC 60060-1.

• Journal of Gastric Cancer
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• v.12 no.1
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• pp.7-12
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• 2012

More than ten years have passed since the sentinel node (SN) concept for gastric cancer surgery was first discussed. Less invasive modified surgical approaches based on the SN concept have already been put into practice for malignant melanoma and breast cancer, however the SN concept is not yet placed in a standard position in gastric cancer surgery even after two multi-institutional prospective clinical trials, the Japan Clinical Oncology Group trial (JCOG0302) and the Japanese Society for Sentinel Node Navigation Surgery (SNNS) trial. What is the problem in the clinical application of the SN concept to gastric cancer surgery? There is no doubt that we need reliable indicator(s) to determine with certainty the absence of metastasis in the lymph nodes in order to avoid unnecessary lymphadenectomy. There are several matters of debate in performing the actual procedure, such as the type of tracer, the site of injection, how to detect and harvest, how to detect metastases of SNs, and learning period. These issues have to be addressed further to establish the most suitable procedure. Novel technologies such as indocyanine green (ICG) fluorescence imaging and one-step nucleic acid amplification (OSNA) may overcome the current difficulties. Once we know what the problems are and how to tackle them, we can pursue the goal.

• The Plant Pathology Journal
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• v.18 no.5
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• pp.271-278
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• 2002

The effect of five plant population densities [5 (D$_1$), 10 (D$_2$), 20 (D$_3$), 30 (D$_4$), and 40 (D$_{5}$) plants/m$^2$] of four groundnut cultivars [ICGV 86699, ICG (FDRS) 10, ICGS 11 and TMV 2] and fungicide application (Kavach, chlorothalonil) to manage late leaf spot (LLS) and rust were studied in a field experiment during the 1995 and 1996 rainy seasons. LLS and rust severities were low in fungicide sprayed plots in all the cultivars irrespective of plant densities. Severities of LLS and rust, and percentage defoliation caused by LLS were significantly more in higher plant densities (D$_4$, D$_{5}$) than in lower plant densities (D$_1$, D$_2$, D$_3$) in fungicide sprayed and unsprayed plots in all the cultivars. All the cultivars gave significantly higher haulm and pod yields in fungicide sprayed plots than in unsprayed plots. Haulm and pod yields were significantly higher in higher plant densities than in lower plant densities. A combination of higher plant densities (D$_4$, D$_{5}$) and fungicide protection against LLS and rust gave maximum yield.yield.

• Medical Lasers
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• v.10 no.3
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• pp.123-131
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• 2021

Optical imaging modalities with properties of real-time, non-invasive, in vivo, and high resolution for image-guided surgery have been widely studied. In this review, we introduce two optical imaging systems, that could be the core of image-guided surgery and introduce the system configuration, implementation, and operation methods. First, we introduce the optical coherence tomography (OCT) system implemented by our research group. This system is implemented based on a swept-source, and the system has an axial resolution of 11 ㎛ and a lateral resolution of 22 ㎛. Second, we introduce a fluorescence imaging system. The fluorescence imaging system was implemented based on the absorption and fluorescence wavelength of indocyanine green (ICG), with a light-emitting diode (LED) light source. To confirm the performance of the two imaging systems, human malignant melanoma cells were injected into BALB/c nude mice to create a xenograft model and using this, OCT images of cancer and pathological slide images were compared. In addition, in a mouse model, an intravenous injection of indocyanine green was used with a fluorescence imaging system to detect real-time images moving along blood vessels and to detect sentinel lymph nodes, which could be very important for cancer staging. Finally, polarization-sensitive OCT to find the boundaries of cancer in real-time and real-time image-guided surgery using a developed contrast agent and fluorescence imaging system were introduced.

• Journal of Biomedical Engineering Research
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• v.36 no.1
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• pp.16-21
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• 2015

Indocyanine green(ICG) and 5-aminolevulinic acid(5-ALA) have been widely used to mark blood vessels or tumors. However, fluorescent dye detection systems were designed to use one type of dyes only. In this study, we proposed a detection system capable of detecting Indocyanine green and 5-aminolevulinic acid. Multiple filters and light sources are integrated into a single system. In this study, we performed analysis of fluorescent dyes and configured a detection system. During the analysis, it was found that Indocyanine green and 5-aminolevulinic acid have the maximum intensity at $40{\mu}M$. We designed light source for fluorescent dyes and conducted compatibility test using a commercial surgical microscope. The fluorescent dye detection system was configured based on the experimental results. The developed system successfully detects Indocyanine green and 5-aminolevulinic acid. Therefore, more efficient surgical operations can be achieved using both fluorescent dyes at the same time. We expect that the developed system can increase the survival rate of patients.