• Title/Summary/Keyword: IAA

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In vitro shoot regeneration from leaf tissue of "Whangkeumbae" pear(Pyrus pyrifolia Nakai) (황금배(Pyrus pyrifolia Nakai) 잎 조직으로부터 기내 신초 재분화)

  • Chun, Jae An;Do, Kyung Ran;Kim, Se Hee;Cho, Kang-Hee;Kim, Hyun Ran;Hwang, Hae Sung;Shin, Il Sheob
    • Journal of Plant Biotechnology
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    • v.39 no.4
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    • pp.288-294
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    • 2012
  • In order to establish an efficient adventitious shoot regeneration conditions from leaf explants for Asian pear 'Whangkeumbae', the effect of concentration and kinds of plant growth regulator and carbon source was investigated. Leaf explants of cultures grown on Murashige and Skoog (MS) medium containing 8 g/L plant agar were used. When the medium contained 0.25 mg/L thidiazuron (TDZ) and 0.3 mg/L indolebutyric acid (IBA), the adventitious shoot regeneration rate (ASRR) was greater as 61.1% than others treated and higher TDZ concentrations (2.5 and 5 mg/L) treatment significantly reduced the ASRR. As the effect of IBA and indoleacetic acid (IAA) concentration on the ASRR, 0.5 mg/L TDZ plus different concentration of IAA exhibited relatively high ASRR and 0.5 mg/L TDZ plus 0.3 mg/L IAA showed the highest ASRR of 76.7%. Also the effect of sucrose and sorbitol as carbon source on regeneration was examined. The highest ASRR and the most shoots per explants averaged 94.4% and 3.49 by treatment of 30 mg/L sorbitol, respectably. Sorbitol is considered better carbon source than sucrose for shoot regeneration of 'Whangkeumbae' pear.

Medium Composition and Growth Regulator on Organogenesis Platycodon grandiflorum (Jacq.) A. DC. with Yellow Green Petals ('녹색 꽃잎 도라지'의 기관분화에 미치는 배지조성 및 생장조절제의 영향)

  • Kwon, Soo Jeong;Cho, Kab Yeon;Kim, Hag Hyun
    • Korean Journal of Plant Resources
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    • v.27 no.1
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    • pp.43-50
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    • 2014
  • This study was carried out to determine the optimal medium composition and growth regulators for the micropropagation of Platycodon grandiflorum (Jacq.) A. DC. Nodes containing yellow green petals were used as plant materials to execute the study. The best performance of adventitious root development was found in 1/4 strength of MS basal salt and the growth was satisfactory in the concentration of 1/2 MS medium. The best condition for adventitious root development and growth was observed in the higher concentration (5%) of sucrose and activated charcoal free 1/4MS medium respectively. Adventitious roots were developed at the controlled culture medium at pH 4.8 with a tendency of suppression with higher levels of pH. However, it was prevailed that the development and growth depended on the concentration of agar. The lower concentration of agar (0.4%) was performed better than that of higher concentration (1.2%), whereas the agar concentration (0.4%) showed the best performance for the development and growth of adventitious roots. For the development of shoots containing node, BA combined with IAA was more effective than kinetin with IAA or NAA. The highest shoot development (3.9 shoots per explant) was performed on MS medium supplemented with 0.1 mg/L BA and 0.5 mg/L IAA.

In Vitro Propagation of Lilium Asiatic Hybrid 'Hae Hwa' via the Formation of Shoot Clusters (신초 Cluster 형성에 의한 Lilium Asiatic Hybrid 'Hae Hwa'의 기내번식)

  • Han, Bong-Hee;Yu, Hee-Ju;Yae, Byeoung-Woo;Goo, Dae-Hoe
    • Journal of Plant Biotechnology
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    • v.29 no.1
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    • pp.19-23
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    • 2002
  • This experiment was conducted to micropropagate bulblets via shoot cluster formation and massproduce normal bulblets from the sections of proliferated shoot clusters in Lilium asiatic hybrid 'Hae Hwa'. The induction of shoot clusters from the culture of bulblet sections was more effective than that of bulb scales on MS medium with 1.0 mg/L BA and 0.5 mg/L IAA. Proliferation of shoot clusters from the formed shoot cluster sections was the most favorable on medium containing 5.0 mg/L BA and 0.5 mg/L IAA. The formation and the growth of bulblets from shoot cluster sections were achieved effectively on medium with 60∼90 g/L sucrose. The leaves derived from shoot clusters grew vigorously but the bulblets from shoot clusters grew very poor in 5L air-lift bioreactor culture. By the addition of 30 mL fresh liquid medium containing doulble strength MS salts, 250 g/L sucrose and 5 g/L activated charcoal after 8 weeks in the shoot cluster culture on MS medium with 5.0 mg/L BA and 0.5 mg/L IAA, the number of bulblets was increased in light condition, but the growth of bulblets was not affected by light. Bulblet production was possible with the bulblet product at 53 to 68 mg in fresh weight by liquid medium addition after the proliferation of shoot cluster.

Resource Clustering Simulator for Desktop Virtualization Based on Intra Cloud (인트라 클라우드 기반 데스크탑 가상화를 위한 리소스 클러스터링 시뮬레이터)

  • Kim, Hyun-Woo
    • KIPS Transactions on Software and Data Engineering
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    • v.8 no.1
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    • pp.45-50
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    • 2019
  • With the gradual advancement of IT, passive work processes are automated and the overall quality of life has greatly improved. This is made possible by the formation of an organic topology between a wide variety of real-life smart devices. To serve these diverse smart devices, businesses or users are using the cloud. The services in the cloud are divided into Infrastructure as a Service (IaaS), Platform as a Service (PaaS) and Software as a Service (SaaS). SaaS runs on PaaS, and PaaS runs on IaaS. Since IaaS is the basis of all services, an algorithm is required to operate virtualization resources efficiently. Among them, desktop resource virtualization is used for resource high availability of unused state time of existing desktop PC. Clustering of hierarchical structures is important for high availability of these resources. In addition, it is very important to select a suitable algorithm because many clustering algorithms are mainly used depending on the distribution ratio and environment of the desktop PC. If various attempts are made to find an algorithm suitable for desktop resource virtualization in an operating environment, a great deal of power, time, and manpower will be incurred. Therefore, this paper proposes a resource clustering simulator for cluster selection of desktop virtualization. This provides a clustering simulation to properly select clustering algorithms and apply elements in different environments of desktop PCs.

Specificity of Auxin Action on Ethylene Production in Corn Coleoptile Segments (옥수수(Zea mays L.) 자엽초 절편에서 에틸렌 생성에 대한 오옥신의 작용 특성)

  • 윤인선
    • Journal of Plant Biology
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    • v.34 no.4
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    • pp.325-330
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    • 1991
  • The ability of several auxin analogs to induce ethylene production was tested in the corn coleoptile. The synthetic auxins 1-naphthaleneacetic acid (1-NAA) and 2, 4-dichlorophenoxyacetic acid (2, 4-D) had strong stimulatory effects on ethylene induction surpassing that of IAA. Both 2-naphthalaneacetic acid (2-NAA) and 2, 6-dichlorophenoxy acetic acid (2, 6-D), structural analogs of these auxins, respectively, were found to be inactive. Treatment with NPA, a strong inhibitor of polar auxin transport, led to drastic increase in IAA-induced ethylene production while it has bo effect on ethylene production induced by 1-NAA. A positive correlative existed between intracellular auxin level and ethylene production.

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Physiological and Biochemical Properties of Isolates of Rhizobia from Soybean (콩에서 분리한 근류균의 생리, 생화학적 특성)

  • 박기선;최재을
    • Korean Journal Plant Pathology
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    • v.12 no.3
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    • pp.351-357
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    • 1996
  • 콩으로부터 분리한 140균주의 근류균은 25균주(17.9%)가 Rhizobium fredii로 115 균주(82.1%)가 Brady-rhizobium japonicum으로 동정되었다. R. fredii에 속하는 분리 균주의 생존 pH 범위는 4..5∼9.0이었고 B. japonicum의 생육 pH 범위는 5.5∼8.0로 비교적 좁게 나타났다. B. japonicum에 속하는 98균주 중에서는 53균주(54%)가 IAA를 생산하지 않는 GT I group으로, 45균주(46%)는 IAA를 생산하는 GT II group으로 명확하게 구분되었으며, 항생물질에 대한 내성 유무에 의해 10개의 group으로 구분되었다.

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Arbitration in Singapore

  • Mardiani, Henny
    • Journal of Arbitration Studies
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    • v.16 no.3
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    • pp.217-230
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    • 2006
  • Singapore is a dual-track arbitration regime. Where seat of arbitration is Singapore, the governing procedural law for domestic arbitration is AA and for international arbitration is IAA. The parties may by agreement opt-out of and opt-into a specific regime. SIAC is a leading arbitral institution in Singapore. It offers wide range of services: administer arbitration proceedings, arrangement of logistics for arbitration hearing, appointment of arbitral tribunal for ad hoc arbitration in Singapore as well as registry and authentication of arbitral awards.

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지치 세포 배양에 의한 시코닌계 색소생산

  • Park, Su-Nam;Lee, Hyeon-Tae;Han, Gi-Tae
    • Journal of the Society of Cosmetic Scientists of Korea
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    • v.13 no.1
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    • pp.7-14
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    • 1987
  • Production of shikonin derivatives through cell suspension culture of Lithospermum erythrorhizon was investigated. Optimal concentrations of IAA and kinetin on the growth of cell suspension were 0.2 and 0.1 ppm respectively. Pigment content was markedly increased when aluminum oxide was added to the production medium and its optimal concentration was 1.5g/70ml medium. The most effective concentration of IAA was 0.5 ppm and the production of pigment did not depend on the kinetin concentration.

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