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http://dx.doi.org/10.7732/kjpr.2014.27.1.043

Medium Composition and Growth Regulator on Organogenesis Platycodon grandiflorum (Jacq.) A. DC. with Yellow Green Petals  

Kwon, Soo Jeong (Department of Flower Floral Plant Coordi & Landscape Architecture, Woosong College)
Cho, Kab Yeon (Department of Food Nutrition and Cookery, Woosong College)
Kim, Hag Hyun (Department of Flower Floral Plant Coordi & Landscape Architecture, Woosong College)
Publication Information
Korean Journal of Plant Resources / v.27, no.1, 2014 , pp. 43-50 More about this Journal
Abstract
This study was carried out to determine the optimal medium composition and growth regulators for the micropropagation of Platycodon grandiflorum (Jacq.) A. DC. Nodes containing yellow green petals were used as plant materials to execute the study. The best performance of adventitious root development was found in 1/4 strength of MS basal salt and the growth was satisfactory in the concentration of 1/2 MS medium. The best condition for adventitious root development and growth was observed in the higher concentration (5%) of sucrose and activated charcoal free 1/4MS medium respectively. Adventitious roots were developed at the controlled culture medium at pH 4.8 with a tendency of suppression with higher levels of pH. However, it was prevailed that the development and growth depended on the concentration of agar. The lower concentration of agar (0.4%) was performed better than that of higher concentration (1.2%), whereas the agar concentration (0.4%) showed the best performance for the development and growth of adventitious roots. For the development of shoots containing node, BA combined with IAA was more effective than kinetin with IAA or NAA. The highest shoot development (3.9 shoots per explant) was performed on MS medium supplemented with 0.1 mg/L BA and 0.5 mg/L IAA.
Keywords
Organogenesis; Adventitious root; Medium Composition; Culture Materials; Micropropagation; Sucrose;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
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