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http://dx.doi.org/10.5010/JPB.2002.29.1.019

In Vitro Propagation of Lilium Asiatic Hybrid 'Hae Hwa' via the Formation of Shoot Clusters  

Han, Bong-Hee (National Horticultural Research Institute, RDA)
Yu, Hee-Ju (National Horticultural Research Institute, RDA)
Yae, Byeoung-Woo (National Horticultural Research Institute, RDA)
Goo, Dae-Hoe (National Horticultural Research Institute, RDA)
Publication Information
Journal of Plant Biotechnology / v.29, no.1, 2002 , pp. 19-23 More about this Journal
Abstract
This experiment was conducted to micropropagate bulblets via shoot cluster formation and massproduce normal bulblets from the sections of proliferated shoot clusters in Lilium asiatic hybrid 'Hae Hwa'. The induction of shoot clusters from the culture of bulblet sections was more effective than that of bulb scales on MS medium with 1.0 mg/L BA and 0.5 mg/L IAA. Proliferation of shoot clusters from the formed shoot cluster sections was the most favorable on medium containing 5.0 mg/L BA and 0.5 mg/L IAA. The formation and the growth of bulblets from shoot cluster sections were achieved effectively on medium with 60∼90 g/L sucrose. The leaves derived from shoot clusters grew vigorously but the bulblets from shoot clusters grew very poor in 5L air-lift bioreactor culture. By the addition of 30 mL fresh liquid medium containing doulble strength MS salts, 250 g/L sucrose and 5 g/L activated charcoal after 8 weeks in the shoot cluster culture on MS medium with 5.0 mg/L BA and 0.5 mg/L IAA, the number of bulblets was increased in light condition, but the growth of bulblets was not affected by light. Bulblet production was possible with the bulblet product at 53 to 68 mg in fresh weight by liquid medium addition after the proliferation of shoot cluster.
Keywords
BA; bioreactor; liquid medium addition; sucrose;
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