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In vitro shoot regeneration from leaf tissue of "Whangkeumbae" pear(Pyrus pyrifolia Nakai)

황금배(Pyrus pyrifolia Nakai) 잎 조직으로부터 기내 신초 재분화

  • Chun, Jae An (National Institute of Horticultural & Herbal Science, RDA) ;
  • Do, Kyung Ran (National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Se Hee (National Institute of Horticultural & Herbal Science, RDA) ;
  • Cho, Kang-Hee (National Institute of Horticultural & Herbal Science, RDA) ;
  • Kim, Hyun Ran (National Institute of Horticultural & Herbal Science, RDA) ;
  • Hwang, Hae Sung (National Institute of Horticultural & Herbal Science, RDA) ;
  • Shin, Il Sheob (National Institute of Horticultural & Herbal Science, RDA)
  • 천재안 (농촌진흥청 국립원예특작과학원) ;
  • 도경란 (농촌진흥청 국립원예특작과학원) ;
  • 김세희 (농촌진흥청 국립원예특작과학원) ;
  • 조강희 (농촌진흥청 국립원예특작과학원) ;
  • 김현란 (농촌진흥청 국립원예특작과학원) ;
  • 황해성 (농촌진흥청 국립원예특작과학원) ;
  • 신일섭 (농촌진흥청 국립원예특작과학원)
  • Received : 2012.11.09
  • Accepted : 2012.11.27
  • Published : 2012.12.31

Abstract

In order to establish an efficient adventitious shoot regeneration conditions from leaf explants for Asian pear 'Whangkeumbae', the effect of concentration and kinds of plant growth regulator and carbon source was investigated. Leaf explants of cultures grown on Murashige and Skoog (MS) medium containing 8 g/L plant agar were used. When the medium contained 0.25 mg/L thidiazuron (TDZ) and 0.3 mg/L indolebutyric acid (IBA), the adventitious shoot regeneration rate (ASRR) was greater as 61.1% than others treated and higher TDZ concentrations (2.5 and 5 mg/L) treatment significantly reduced the ASRR. As the effect of IBA and indoleacetic acid (IAA) concentration on the ASRR, 0.5 mg/L TDZ plus different concentration of IAA exhibited relatively high ASRR and 0.5 mg/L TDZ plus 0.3 mg/L IAA showed the highest ASRR of 76.7%. Also the effect of sucrose and sorbitol as carbon source on regeneration was examined. The highest ASRR and the most shoots per explants averaged 94.4% and 3.49 by treatment of 30 mg/L sorbitol, respectably. Sorbitol is considered better carbon source than sucrose for shoot regeneration of 'Whangkeumbae' pear.

본 연구는 '황금배'('Whangkeumbae')의 엽 절편체로부터 형질전환 체계 확립을 위한 효율적인 재분화 체계 확립을 위해 식물 생장조절제와 주요 탄소원인 sucrose, sorbitol이 식물체 재분화에 미치는 영향을 조사하기 위해 수행되었다. 잎 절편체를 MS 배지에 TDZ 농도를 각각 0.1, 0.25, 0.5, 1, 2.5, 5 mg/L으로 하여 IBA 0.3 mg/L을 혼용 처리한 결과 TDZ 0.25 mg/L 농도에서 61.1%의 가장 양호한 재분화율을 보였으며 TDZ 2.5 mg/L 이상의 농도에서는 신초의 재분화율이 급격히 감소하였다. IBA와 IAA 농도에 의한 신초의 재분화율은 TDZ 0.5 mg/L와 IAA 혼용 처리한 구에서 전체적으로 높은 신초의 재분화율을 보였으며 특히 TDZ 0.5 mg/L + IAA 0.3 mg/L 처리구에서 76.7%의 가장 높은 재분화율을 보였다. 서로 다른 탄소원으로써 sucrose와 sorbitol의 영향에 대하여 15 g/L, 30 g/L 농도로 처리한 결과 sorbitol 30 mg/L에서 가장 높은 재분화율을 보였고 또한 절편체 당 신초의 수도 3.5개로 가장 양호하였다. 따라서 sorbitol이 sucrose보다 '황금배'의 신초의 재분화에 있어서 효과적이었다.

Keywords

References

  1. Ahmad T, Abbasi NA, Hafiz IA, Ali A (2007) Comparison of sucrose and sorbitol as main carbon energy sources in micropropagation of peach rootstock GF-677. Pak J Bot 39:1269-1275
  2. Bell RL (2003) Interaction of genotype and auxin affecting regeneration of pear. Hortscience 38:750
  3. Bell RL, Scorza R, Lomberk D (2012) Adventitious shoot regeneration of pear (Pyrus spp.) genotypes. Plant Cell Tissue Organ Cult 108:229-236 https://doi.org/10.1007/s11240-011-0034-4
  4. Brown DCW, Leung DWM, Thorpe TA (1979) Osmotic requirement for shoot formation in tabacco callus. Physiol Plant 46:36-41 https://doi.org/10.1111/j.1399-3054.1979.tb03182.x
  5. Caboni E, Tonelli MG, Lauri P, Angeli SD, Damiano C (1999) In vitro shoot regeneration from leaves of wild pear. Plant Cell Tissue Organ Cult 59:1-7 https://doi.org/10.1023/A:1006351214343
  6. Caboni E. Lauri P. D`Angeli S (2000) In vitro plant regeneration from callus of shoot apices in apple shoot culture. Plant Cell Rep 19:755-760 https://doi.org/10.1007/s002999900189
  7. Chevreau E, Leblay C (1993) The effect of mother plant pretreatment and explants choice on regeneration from in vitro pear leaves. Acta Hort 336: 263-268
  8. Chevreau E, Mourgues F, Martine N, Chevalier M (1997) Effect of gelling agents and antibiotics on adventitious bud regeneration from in vitro leaves of pear. In Vitro Cell Dev Biol Plant 33:173-179 https://doi.org/10.1007/s11627-997-0017-7
  9. Chevreau E, Skirvin RM, Abu-Qaoud HA, Korban SS, Sullivan JG (1989) Adventitious shoot regeneration from leaf tissue of three pear (Pyrus sp.). Plant Cell Rep 7:688-691
  10. Feng X, Zhao P, Hao J, Hu J, Kang D, Wang H (2011) Effects of sorbitol on expression of genes involved in regeneration of upland rice (Oryza sativa L.). Plant Cell Tissue Organ Cult 106:455-463 https://doi.org/10.1007/s11240-011-9943-5
  11. Garcia R, Pacheco G, Falca˜o G, Borges G, Mansur E (2011) Influence of type of explant, plant growth regulators, salt composition of basal medium, and light on callogenesis and regeneration in Passiflora suberosa L. (Passifloraceae) Renata. Plant Cell Tissue Organ Cult 106:47-54 https://doi.org/10.1007/s11240-010-9892-4
  12. Geng P, La H, Wang H, Stevens EJC (2008) Effect of sorbitol concentration on regeneration of embryogenic calli in upland rice varieties (Oryza sativa L.). Plant Cell Tissue Organ Cult 92:303-313 https://doi.org/10.1007/s11240-007-9321-5
  13. Hassan MU, Ahmed Z, Munir M, Malik SI and Shahzad K (2009) Effect of sorbitol in callus induction and plant regeneration in wheat. Afr J Biotechnol 8:6529-6535
  14. Hennayake CK, Dissanayake K, Matsuda N, Takasaki T, Nakanishi T (2003) An Efficient and reproducible in vitro plant regeneration from leaf discs in pear cultivars (Pyrus spp). Plant Biotechnol 20:283-289 https://doi.org/10.5511/plantbiotechnology.20.283
  15. Huetteman CA, Preece JE (1993) Thidiazuron: a potent cytokine for woody plant tissue culture. Plant Cell Tissue Organ Cult 33:105-119 https://doi.org/10.1007/BF01983223
  16. Kadota M, Imizu K, Hirano T (2001) Double-phase in vitro culture using sorbitol increases shoot proliferation and reduces hyperhydricity in Japanese pear. Sci Hort 89:207-215 https://doi.org/10.1016/S0304-4238(00)00234-X
  17. Kadota M, Niimi Y (2003) Effect of cytokinin types and their concentrations on shoot proliferation and hyperhydricity in in vitro pear cultivar shoots. Plant Cell Tissue Organ Cult 72:261-265 https://doi.org/10.1023/A:1022378511659
  18. Keulemans J, de Witte K (1994) Plant regeneration from cotyledons and embryonic axes in apple: Sites of reaction and effect of pre-culture in the light. Euphytica 77:135-139 https://doi.org/10.1007/BF02551476
  19. Kim JH, Song KJ (2010) Current status and outlook on genetic transformation of fruit trees in Korea. J Plant Biotechnol 37:408-413 https://doi.org/10.5010/JPB.2010.37.4.408
  20. Lane WD (1979) Regeneration of pear plants from shoot meristem-TIP. Plnat Sci Lett 16:337-342 https://doi.org/10.1016/0304-4211(79)90046-4
  21. Lane WD, Iketani H, Hayashi T (1998) Shoot regeneration from cultured leaves of Japanese pear (Pyrus pyrifolia). Plant Cell Tissue Organ Cult 54:9-14 https://doi.org/10.1023/A:1006032707849
  22. Leblay C, Chevreay E, Raboin LM (1991) Adventitious shoot regeneration from in vitro leaves of several pear cultivars (pyrus communis L.). Plant Cell Tissue Organ Cult 25:99-105
  23. Lee CH, Kim CS, Kim SB, Noh YM, Han DH, Ban SJ, Kang SK, Kang SJ (2002) Development of efficient regeneration system for Pyrus pyrifolia cv. Nitaka from leaf segments. J Kor Soc Hort Sci 43:271-274
  24. Lemos EEP, Baker DA (1998) Shoot regeneration in response to carbon source on intermodal explants of Annona muricata L. Plant Growth Regul 25:105-112 https://doi.org/10.1023/A:1006058301707
  25. Marino G, Bertazza G, Magnanini E, Altan AD (1993) Comparative effects of sorbitol and sucrose as main carbon energy sources in micropropagation of apricot. Plant Cell Tissue Organ Cult 34:235-244 https://doi.org/10.1007/BF00029712
  26. Mukherjee SK, Rathinasabapathi B, Gupta N (1991) Low sugar and osmotic requirements for shoot regeneration from leaf piece of Solanum melongena L. Plant Cell Tissue Organ Cult 25:13-16 https://doi.org/10.1007/BF00033906
  27. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol Plant 15:473-497 https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
  28. Ochatt SJ, Power JB (1988) Plant regeneration from mesophyll protoplasts of Williams` Bon chretien (syn. Bartlett) pear (Pyrus communis L.) Plant Cell Rep 7:587-589 https://doi.org/10.1007/BF00272764
  29. Predieri S, Fasolo F (1989) High-frequency shoot regeneration from leaves of the apple rootstock M26 (Malus pumila Mill). Plant Cell Tissue Organ Cult 17:133-142 https://doi.org/10.1007/BF00046858
  30. Saito A, Suzuki M (1999) Plant regeneration from meristem-derived callus protoplasts of apple (Malus x domestica cv. 'Fuji'). Plant Cell Rep 18:549-553 https://doi.org/10.1007/s002990050620
  31. Sotiropoulos TE, Molassiotis AN, Mouhtaridou GI, Papadakis I, Dimassi KN, Therios IN, Diamantidis G (2006) Sucrose and sorbitol effects on shoot growth and proliferation in vitro, nutritional status and peroxidase and catalase isoenzymes of M 9 and MM 106 Apple (Malus domestica Borkh.) rootstocks. Europ J Hort Sci 71:114-119
  32. Swedlund B, Locy RD (1993) Sorbitol as the primary carbon source for the growth of embryogenic callus of Maize. Plant Physiol 103:1339-1346 https://doi.org/10.1104/pp.103.4.1339
  33. Zhang P, Yu ZY, Cheng ZM, Zhang Z, TAO JM (2011) In vitro explants regeneration of the grape 'Wink' (Vitis vinifera L.) J Plant Breed Crop Sci 3:276-282

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