• Title/Summary/Keyword: I3A

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Molecular Cloning of Insulin-like Growth Factor-I (IGF-I) and IGF-II Genes of Marine Medaka (Oryzias dancena) and Their Expression in Response to Abrupt Transfer from Freshwater to Seawater

  • Kang, Yue-Jai;Kim, Ki-Hong
    • Fisheries and Aquatic Sciences
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    • v.13 no.3
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    • pp.224-230
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    • 2010
  • Growth hormone (GH) is known as one of the main osmoregulators in euryhaline teleosts during seawater (SW) adaptation. Many of the physiological actions of GH are mediated through insulin-like growth factor-I (IGF-I), and the GH/IGF-I axis is associated with osmoregulation of fish during SW acclimation. However, little information is available on the response of fish IGF-II to hyperosmotic stress. Here we present the first cloned IGF-I and IGF-II cDNAs of marine medaka, Oryzias dancena, and an analysis of the molecular characteristics of the genes. The marine medaka IGF-I cDNA is 1,340 bp long with a 257-bp 5' untranslated region (UTR), a 528 bp 3' UTR, and a 555-bp open reading frame (ORF) encoding a propeptide of 184 amino acid (aa) residues. The full-length marine medaka IGF-II cDNA consists of a 639 bp ORF encoding 212 aa, a 109 bp 5' UTR, and a 416 bp 3' UTR. Homology comparison of the deduced aa sequences with other IGF-Is and IGF-IIs showed that these genes in marine medaka shared high structural homology with orthologs from other teleost as well as mammalian species, suggesting high conservation of IGFs throughout vertebrates. The IGF-I mRNA level increased following transfer of marine medaka from freshwater (FW) to SW, and the expression level was higher than that of the control group, which was maintained in FW. This significantly elevated IGF-I level was maintained throughout the experiment (14 days), suggesting that in marine medaka, IGF-I is deeply involved in the adaptation to abrupt salinity change. In contrast to IGF-I, the increased level of marine medaka IGF-II mRNA was only maintained for a short period, and quickly returned a level similar to that of the control group, suggesting that marine medaka IGF-II might be a gene that responds to acute stress or one that produces a supplemental protein to assist with the osmoregulatory function of IGF-I during an early phase of salinity change.

Identification of Four Cyst Nematodes using PCR-RFLP in Korea (PCR-RFLP를 이용한 국내 분포 씨스트선충 4종의 동정)

  • Ko, Hyoung-Rai;Kang, Heonil;Park, Eun-Hyoung;Kim, Eun-Hwa;Lee, Jae-Kook
    • Korean Journal of Organic Agriculture
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    • v.27 no.3
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    • pp.353-363
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    • 2019
  • To identify four cyst nematodes (Heterodera schachtii, H. trifolii, H. glycines, H. sojae) that are economically important plant-parasitic nematodes in Korea, restriction fragment length polymorphism (RFLP) by 8 endonucleases (PstI, VspI, AlwI, RsaI, MvaI, EcoRI, Eco72I, Hinf I) was performed based on sequence difference of mitochondrial DNA cytochrome c oxidase subunit I (COI) gene. As a result, species-specific DNA band patterns by RsaI endonuclease were observed in H. schachtii. The specific patterns was in H. trifolii by 3 endonucleases (VspI, AlwI, Hinf I), and was in H. glycines by Hinf I. While, H. sojae was not digested by 4 endonuclease (VspI, AlwI, RsaI, Hinf I). This study showed that four cyst nematodes could be distinguished using RFLP by 4 endonucleases (RsaI, VspI, AlwI, Hinf I) based on the sequence difference of COI gene.

Forest Vegetation and Flora of Mt. Kasan - Phytosociological Analysis - (가산일대(架山一帶)의 삼림식생(森林植生)과 식물상(植物相) - 식물사회학적(植物社會學的) 분석(分析) -)

  • Kim, Weon Ho;Bae, Kwan Ho;Cho, Hyun Je;Hong, Sung Cheon
    • Journal of Korean Society of Forest Science
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    • v.79 no.1
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    • pp.42-55
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    • 1990
  • The forest communities of this area were studied phytosociologically by the method of Z$\ddot{u}$rich-Montpellier school. 1) Forest vegetation in this area divided into mountain forest(Pinus densiflora-Alnus hirsuta community) and plateau(Basin) forest(Weigela subsessilis-Athyrium yokoscense community). 2) Mountain forest(I. Pinus densiflora-Alnus hirsuta community) divided into I-A. Carpinus laxiflora group, I-B. Acer mono group., I-A. Carpinus laxiflora group is further subdivided into I-A-1. Styrax obassia subgroup, I-A-2. Lindera erythrocarpa subgroup, I-A-3. Typical subgroup, I-A-4. Zanthoxylum schinifolium subgroup. 3) Plateau(Basin) forest(II. Weigela subsessilis-Athyrium yokoscense community.) divided into II-A. Carpinus laxiflora group, II-B. Carpinus cordata-Actinidia arguta group, II-C. Typical group. II-A. Carpinus laxiflora group is further subdivided into II-A-1. Symplocos chinensis for. pilosa subgroup, II-A-2. Typical subgroup. II-A-3. Acer mono subgroup. The flora of vasicula plants distributed in Mt. Kasan area was 105 families 350 genera, 515 species, 87 varieties, 2 subspecies and 12 formae in total 616 taxa (This amount to some 64.7% of the whole plant (952 kinds) in Mt. Palgong nature area.).

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Antibacterial Effects of Pseudomonas aeruginosa MB I-3 against Listonella anguillarum (어류 병원세균, Listonella anguillarum에 대한 Pseudomonas aeruginosa MB I-3의 항균 효과)

  • Lee, Su-Jung;Youn, I Na;Kim, Jin-Do;Lee, Jung Sick;Kim, Eunheui
    • Journal of fish pathology
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    • v.27 no.1
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    • pp.17-24
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    • 2014
  • To study the possible use of probiotics in fish farming, The in vitro and in vivo antibacterial effects of Pseudomonas aeruginosa MB I-3 (MB I-3) against the fish pathogenic bacterium Listonella anguillarum were evaluated. The inhibitory effects of MB I-3 against vibrios were investigated by the double layer method and the co-culture. The results showed that MB I-3 inhibited the growth of pathogenic vibrios including Listonella anguillarum, Vibrio alginolyticus, Vibrio cholerae, Vibrio fluvialis, Vibrio furnissii, Vibrio harveyi, Vibrio parahaemolyticus and Vibrio vulnificus. Extracellular substances obtained from the cultural supernatant of MB I-3 by ethyl acetate extraction showed inhibitory effects on L. anguillarum. The antibacterial substance of MB I-3 was evaluated to destroy the cell membrane of L. anguillarum in electron micrographs. The probiotic effects of MB I-3 was tested by exposing olive flounder (Paralichthys olivaceus) fry to L. anguillarum with or without MB I-3. The cumulative mortality of olive flounder fry infected with L. anguillarum was 24% in the group with MB I-3, while it was 46% in the control group without MB I-3. These results indicate that MB I-3 has potential applications as a probiotic for the control of fish pathogenic vibrios in fish rearing system.

APPROXIMATION OF COMMON FIXED POINTS OF NON-SELF ASYMPTOTICALLY NONEXPANSIVE MAPPINGS

  • Kim, Jong-Kyu;Dashputre, Samir;Diwan, S.D.
    • East Asian mathematical journal
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    • v.25 no.2
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    • pp.179-196
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    • 2009
  • Let E be a uniformly convex Banach space and K a nonempty closed convex subset which is also a nonexpansive retract of E. For i = 1, 2, 3, let $T_i:K{\rightarrow}E$ be an asymptotically nonexpansive mappings with sequence ${\{k_n^{(i)}\}\subset[1,{\infty})$ such that $\sum_{n-1}^{\infty}(k_n^{(i)}-1)$ < ${\infty},\;k_{n}^{(i)}{\rightarrow}1$, as $n{\rightarrow}\infty$ and F(T)=$\bigcap_{i=3}^3F(T_i){\neq}{\phi}$ (the set of all common xed points of $T_i$, i = 1, 2, 3). Let {$a_n$},{$b_n$} and {$c_n$} are three real sequences in [0, 1] such that $\in{\leq}\;a_n,\;b_n,\;c_n\;{\leq}\;1-\in$ for $n{\in}N$ and some ${\in}{\geq}0$. Starting with arbitrary $x_1{\in}K$, define sequence {$x_n$} by setting {$$x_{n+1}=P((1-a_n)x_n+a_nT_1(PT_1)^{n-1}y_n)$$ $$y_n=P((1-b_n)x_n+a_nT_2(PT_2)^{n-1}z_n)$$ $$z_n=P((1-c_n)x_n+c_nT_3(PT_3)^{n-1}x_n)$$. Assume that one of the following conditions holds: (1) E satises the Opial property, (2) E has Frechet dierentiable norm, (3) $E^*$ has Kedec -Klee property, where $E^*$ is dual of E. Then sequence {$x_n$} converges weakly to some p${\in}$F(T).

Determinations of the Exposure Rate Using a NaI(Tl) Detector of the Environmental Radiation Monitor (환경방사선감시기의 NaI(Tl) 검출기를 이용한 조사선량률 결정방법)

  • Ji, Young-Yong;Lee, Wanno;Choi, Sang-Do;Chung, Kun Ho;Kang, Mun Ja;Choi, Geun-Sik
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.11 no.3
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    • pp.245-251
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    • 2013
  • The energy band and the G-factor method were compared to determine the exposure rate from the measured spectrum using a NaI(Tl) scintillation detector. First, G-factors of a 3"${\Phi}X3$" NaI(Tl) detector mounted to a EFRD 3300, which means the environmental radiation monitor, in Korea Atomic Energy Research Institute (KAERI) were calculated for several directions of incident photons through the MCNP modeling, and the optimum G-factor applicable to that monitor was then determined by comparing the results both the energy band method and the G-factor method. The results for these spectrometric determinations were also compared with the dose rate from a HPIC radiation monitor around a EFRD 3300. The measured value at the EFRD 3300 based on a 3"${\Phi}X3$" NaI(Tl) detector was $7.7{\mu}R/h$ and its difference was shown about $3{\mu}R/h$, when compared with the results from a HPIC radiation moditor. Since a HPIC is known to be able to measure cosmic rays with the relatively high energy, the difference between them was caused by cosmic rays which were not detected in a 3"${\Phi}X3$" NaI(Tl) detector.

Fabrication and characterization of perovskite CH3NH3Pb1-xSbxI3-3xBr3x photovoltaic devices

  • Yamanouchi, Jun;Oku, Takeo;Ohishi, Yuya;Fukaya, Misaki;Ueoka, Naoki;Tanaka, Hiroki;Suzuki, Atsushi
    • Advances in materials Research
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    • v.7 no.1
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    • pp.73-81
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    • 2018
  • $TiO_2/CH_3NH_3Pb_{1-x}Sb_xI_{3-3x}Br_{3x}-based$ photovoltaic devices were fabricated by a spin-coating method using mixture solutions with $SbBr_3$. Effects of $SbBr3$, CsI or RbBr addition to $CH_3NH_3PbI_3$ precursor solutions on the photovoltaic properties where investigated. The short-circuit current densities and photoconversion efficiencies were improved by adding a small amount of $SbBr_3$, CsI or RbBr to the perovskite phase, which would be due to the doping effect of Sb, Br and Cs/Rb atom at the Pb, I and $CH_3NH3$ sites, respectively.

Growth of Organic/Inorganic MAPbI3 Perovskite Thin Films via Chemical Vapor Deposition (화학 기상 증착법을 이용한 유/무기 MAPbI3 페로브스카이트 박막 성장)

  • Jung, Jang-Su;Eom, Jiho;Pammi, S.V.N.;Yoon, Soon-Gil
    • Journal of the Korean Institute of Electrical and Electronic Material Engineers
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    • v.33 no.4
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    • pp.315-320
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    • 2020
  • Methylammonium lead iodide (MAPbI3) thin films were grown at low temperatures on glass substrates via 3-zone chemical vapor deposition. Lead iodide (PbI2) and lead bis (dipivaloylmethanate) [Pb(dpm)2] precursors were used as lead sources. Due to the high sublimation temperature (~400℃) of the PbI2 precursor, a low substrate temperature could not be constantly maintained. Therefore, MAPbI3 thin films degraded into the PbI2 phase. In contrast, for the Pb(dpm)2 precursor, a substrate temperature of ~120℃ was maintained because the sublimation temperature of Pb(dpm)2 is as low as 130℃ at a high vapor pressure. As a result, high-quality MAPbI3 thin films were successfully grown on glass substrates using Pb(dpm)2. The rms (root-mean-square) roughness of MAPbI3 thin films formed from Pb(dpm)2 was as low as ~19.2 nm, while it was ~22.7 nm for those formed using PbI2. The grain size of the films formed from Pb(dpm)2 was as large as approximately 350 nm.

Thermal and Photochemistry of Methyl Iodide on Ice Film Grown on Cu(111)

  • Sohn, Young-Ku;White, John M.
    • Bulletin of the Korean Chemical Society
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    • v.30 no.7
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    • pp.1470-1474
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    • 2009
  • Thermal and photochemistry of methyl iodide ($CH_3I)\;adsorbed\;on\;D_2O$ ice film on Cu(111) at 100 K were studied using temperature-programmed desorption (TPD) time-of-flight mass spectrometry (TOF-MS), X-ray and ultraviolet photoelectron spectroscopies. On the basis of TPD, multilayer and monolayer $CH_3I$ molecules desorb from $D_2O$ ice layer at 120 and 130 K, respectively. Photo-irradiation at 100 K exhibits dramatic changes in the TPD and I $3d_{5/2}\;XPS\;of\;CH_3I$ on ice film, due to a dramatic dissociation of $CH_3I$. The dissociation is likely activated by solvated electrons transferred from the metal substrate during photo-irradiation. No other photo-initiated reaction products were found within our instrumental detection limit. During photo-irradiation, the $CH_3I$, $CH_3$ and I could be trapped (or solvated) in ice film by rearrangement (and self-diffusion) of water molecules. A newly appeared parent molecular desorption peak at 145 K is attributed to trapped $CH_3I$. In addition, the $CH_3$ and I may diffuse through ice and chemisorb on Cu(111), indicated by TPD and I $d_{5/2}$ XPS taken with photo-irradiation time, respectively. No molecular ejection was found during photo-irradiation at 100 K. The work functions for $CH_3I/Cu(111),\;D_2O/Cu(111)\;and\;CH_3I/D_2$O/Cu(111) were all measured to be about 3.9 eV, 1.0 eV downward shift from that of clean Cu(111).

SYSTEMS OF DERIVATIONS ON BANACH ALGEBRAS

  • Lee, Eun-Hwi
    • Communications of the Korean Mathematical Society
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    • v.12 no.2
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    • pp.251-256
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    • 1997
  • We show that a strong system of derivations ${D_0, D_1,\cdots,D_m}$ on a commutative Banach algebra A is contained in the radical of A if it satisfies one of the following conditions for separating spaces; (1) $\partial(D_i) \subseteq rad(A) and \partial(D_i) \subseteq K D_i(rad(A))$ for all i, where $K D_i(rad(A)) = {x \in rad(A))$ : for each $m \geq 1, D^m_i(x) \in rad(A)}$. (2) $(D^m_i) \subseteq rad(A)$ for all i and m. (3) $\bar{x\partial(D_i)} = \partial(D_i)$ for all i and all nonzero x in rad(A).

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