Browse > Article
http://dx.doi.org/10.11625/KJOA.2019.27.3.353

Identification of Four Cyst Nematodes using PCR-RFLP in Korea  

Ko, Hyoung-Rai (농촌진흥청 국립농업과학원 작물보호과)
Kang, Heonil (부산대학교 식물생명과학과)
Park, Eun-Hyoung (농촌진흥청 국립농업과학원 작물보호과)
Kim, Eun-Hwa (농촌진흥청 국립농업과학원 작물보호과)
Lee, Jae-Kook (국립농업과학원 작물보호과)
Publication Information
Korean Journal of Organic Agriculture / v.27, no.3, 2019 , pp. 353-363 More about this Journal
Abstract
To identify four cyst nematodes (Heterodera schachtii, H. trifolii, H. glycines, H. sojae) that are economically important plant-parasitic nematodes in Korea, restriction fragment length polymorphism (RFLP) by 8 endonucleases (PstI, VspI, AlwI, RsaI, MvaI, EcoRI, Eco72I, Hinf I) was performed based on sequence difference of mitochondrial DNA cytochrome c oxidase subunit I (COI) gene. As a result, species-specific DNA band patterns by RsaI endonuclease were observed in H. schachtii. The specific patterns was in H. trifolii by 3 endonucleases (VspI, AlwI, Hinf I), and was in H. glycines by Hinf I. While, H. sojae was not digested by 4 endonuclease (VspI, AlwI, RsaI, Hinf I). This study showed that four cyst nematodes could be distinguished using RFLP by 4 endonucleases (RsaI, VspI, AlwI, Hinf I) based on the sequence difference of COI gene.
Keywords
cyst nematode; identification; PCR; RFLP;
Citations & Related Records
Times Cited By KSCI : 3  (Citation Analysis)
연도 인용수 순위
1 Amiri, S., S. A. Subbotin, and M. Moens. 2002. Identification of the beet cyst nematode Heterodera schachtii by PCR. Eur. J. Plant Pathol. 108: 497-506.   DOI
2 Barker, K. R. 1985. Nematode extraction and bioassay.: Barker, K. R. C. C. Carter, J. N. Sasser (eds). An advanced treatise on Meloidogyne Volume II: Methodology, North Carolina State University Graphics, US. pp. 19-35.
3 Blok, C. V. and T. O. Powers. 2009. Biochemical and molecular identification.: Perry R. N. M. Moens, J. L. Starr (eds). Root-knot nematodes, eds. CABI International, UK. pp. 98-118.
4 Braun-Kiewnick, A. and Kiewnick, S. 2018. Real-time PCR, a great tool for fast identification, sensitive detection and quantification of important plant-parasitic nematodes. Eur. J. Plant Pathol. 152(2): 271-283.   DOI
5 Cook, R. and G. R. Noel. 2002. Cyst nematodes: Globodera and Heterodera species.: Starr, J. L. R. Cook, J. Bridge (eds). Plant resistance to parasitic nematodes, CABI International, US. pp. 71-105.
6 Derycke, S., T. Remerie, A. Vierstraete, T. Backeljau, J. Vanfleteren, M. Vincx, and T. Moens. 2005. Mitochondrial DNA variation and cryptic speciation within the free-living marine nematode Pellioditis marina. Mar. Ecol. Prog. Ser. 300: 91-103.   DOI
7 Dropkin, V. H. 1980. Introduction.: Dropkin, V. H. (eds). Introduction to plant nematology, A Wiley-Interscience Publication, US. pp. 1-5.
8 Feng, Y., D. Wang, D. Xiao, T. J. Pereira, Y. Xuan, Y. Wang, X. Liu, L. Chen, Y. Duan, and X. Zhu. 2018. Cactodera chenopodiae (Nematoda: Heteroderidae), a new species of cyst nematode parasitizing common lambsquarter (Chenopodium album) in Liaoning, China. Zootaxa 4407(3): 361-375.   DOI
9 Iwahori, H., N. Kanzaki, and K. Futai. 2000. A simple, polymerase chain reaction fragment length polymorphism-aided diagnosis method for pine wilt disease. Forest Pathol. 30: 157-164.   DOI
10 Kang, H., G. Eun, J. Ha, Y. Kim, N. Park, D. Kim, and I. Choi. 2016. New cyst nematode, Heterodera sojae n. sp. (Nematoda: Heteroderidae) from soybean in Korea. J. Nematol. 48(4): 280-289.   DOI
11 Kim, D. G. 2001. Occurrence of root-knot nematodes on fruit vegetables under greenhouse conditions in Korea. Res. Plant Dis. 7(2): 69-79.
12 Lamberti, F., N. Greco, and A. Troccoli. 2007. Control and management of plant-parasitic nematodes.: Saxena G. K. G. Mukerji (eds). Management of nematode and insect-borne plant diseases, The Haworth Press, US. pp. 107-164.
13 Ko, H. R., E. H. Kim, S. J. Kim, J. K. Lee, and W. H. Lee. 2017. Rapid methods to distinguish Heterodera schachtii from Heterodera glycines using PCR technique. Res. Plant Dis. 23(3): 241-248.   DOI
14 Ko, H. R., M. A. Lee, E. H. Kim, S. J. Kim, and J. K. Lee. 2016. Incidence of major plant-parasitic nematodes in main producing areas of strawberry in Korea. Res. Plant Dis. 22(4): 249-256.   DOI
15 Kwon, S. B., D. G. Park, H. S. Won, Y. G. Moon, J. H. Lee, Y. B. Kim, B. G. Choi, H. T. Seo, H. R. Ko, J. K. Lee, and D. W. Lee. 2018. Spread of cyst nematodes in highland chinese cabbage field in Gangwon-do. Korean J. Appl. Entomol. 57(4): 339-345.   DOI
16 Lee, J. K., B. Y. Park, D. R. Choi, and J. W. Heo. 2008. Damage and occurrence of Pratylenchus vulnus and Pratylenchus sp. on spray chrysanthemum, Dendranthema grandiflourm Kitamura. Korean J. Appl. Entomol. 47(4): 473-478.   DOI
17 Mwamula, A. O., H. R. Ko, Y. Kim, Y. H. Kim, J. K. Lee, and D. W. Lee. 2018. Morphological and molecular characterization of Heterodera schachtii and the newly recorded cyst nematode, H. trifolii associated with Chinese cabbage in Korea. Plant Pathol. J. 34(4): 297-307.   DOI
18 Powers, T., T. Harris, R. Giggins, P. Mullin, and K. Powers. 2018. Discovery and identification of Meloidogyne species using COI DNA barcoding. J. Nematol. 50(3): 399-412.   DOI
19 Sharma, S. B. 1998. The cyst nematodes. Kluwer academic publishers, Dordrecht, The Netherlands. p. 452.
20 Subbotin, S. A. 2015. Heterodera sturhani sp. n. from China, a new species of the Heterodera avenae species complex (Tylenchida: Heteroderidae). Russ. J. Nematol. 23(2): 145-152.
21 Subbotin, S. A., F. Toumi, I. H. Elekcioglu, L. Waeyenberge, and Z. T. Maafi. 2018. DNA barcoding, phylogeny and phylogeodraphy of the cyst nematode species of the avenae group from the genus Heterodera (Tylenchida: Heteroderidae). Nematology 20(7): 671-702.   DOI
22 Subbotin, S. A., M. Mundo-Ocampo, and J. G. Baldwin. 2010a. Biology and evolution.: Subbotin, S. A., M. Mundo-Ocampo, J. G. Baldwin (eds). Systematics of cyst nematodes (Nematoda: Heteroderinae) 8A, Koninklijke Brill NV, Netherlands. pp. 43-70.
23 Subbotin, S. A., M. Mundo-Ocampo, and J. G. Baldwin. 2010b. Identification of Heterodera species.: Subbotin, S. A., M. Mundo-Ocampo, J. G. Baldwin (eds). Systematics of cyst nematodes (Nematoda: Heteroderinae) 8B, Koninklijke Brill NV, Netherlands. pp. 9-34.
24 Subbotin, S. A., L. Waeyenberge, and M. Moens. 2000. Identification of cyst forming nematodes of the genus Heterodera (Nematoda: Heteroderidae) based on the ribosomal DNARFLP. Nematology 2(2): 153-164.   DOI