Korean Journal of Organic Agriculture (한국유기농업학회지)

Korean Association of Organic Agriculture (한국유기농업학회)
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Identification of Four Cyst Nematodes using PCR-RFLP in Korea

PCR-RFLP를 이용한 국내 분포 씨스트선충 4종의 동정

  • 고형래 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 강헌일 (부산대학교 식물생명과학과) ;
  • 박은형 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 김은화 (농촌진흥청 국립농업과학원 작물보호과) ;
  • 이재국 (국립농업과학원 작물보호과)
  • Received : 2019.05.10
  • Accepted : 2019.08.06
  • Published : 2019.08.31
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Abstract

To identify four cyst nematodes (Heterodera schachtii, H. trifolii, H. glycines, H. sojae) that are economically important plant-parasitic nematodes in Korea, restriction fragment length polymorphism (RFLP) by 8 endonucleases (PstI, VspI, AlwI, RsaI, MvaI, EcoRI, Eco72I, Hinf I) was performed based on sequence difference of mitochondrial DNA cytochrome c oxidase subunit I (COI) gene. As a result, species-specific DNA band patterns by RsaI endonuclease were observed in H. schachtii. The specific patterns was in H. trifolii by 3 endonucleases (VspI, AlwI, Hinf I), and was in H. glycines by Hinf I. While, H. sojae was not digested by 4 endonuclease (VspI, AlwI, RsaI, Hinf I). This study showed that four cyst nematodes could be distinguished using RFLP by 4 endonucleases (RsaI, VspI, AlwI, Hinf I) based on the sequence difference of COI gene.

Keywords

References

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