• Title/Summary/Keyword: Hypogaea

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Genetic Diversity Assessment and Phylogenetic Analysis of Peanut (Arachis hypogaea L.) in RDA Genebank Collection using SSRs

  • Yi, Jung-Yoon;Lee, Gi-An;Lee, Jeong-Ran;Lee, Myung-Chul;Kang, Man-Jung;Baek, Hyung-Jin;Kim, Chung-Kon
    • Korean Journal of Plant Resources
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    • v.24 no.3
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    • pp.272-279
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    • 2011
  • It is very crucial to evaluate the genetic diversity of peanut genetic resources for identification of peanut germplasm accessions and variety improvement. Cultivated peanut generally has two subspecies, hypogaea and fastigiata. In this study, we identified peanut into three plant types, virginia (var. hypogaea), spanish (var. vulgaris), and valencia (var. fastigiata). Former one belongs to ssp. hypogaea and latter two are involved in ssp. fastigiata. Twenty SSR markers were used to assess the genetic variation of three sets, hypogaea, vulgaris, and fastigiata, respectively. Out of variety-specific SSR primers tried in this study, ten pairs of SSR primers showed polymorphisms. Each accession could be identified by a specific set of polymorphic SSR primers, and allele number was evaluated among accessions, with an average of 6.7 in var. hypogaea and 5.4 in var. vulgaris and fastigiata. For evaluation of genetic diversity, gene diversity ranged from 0.336 to 0.844 and PIC (polymorphism information contents) ranged from 0.324 to 0.827 were investigated. Dendrograms based on genetic distances were constructed, which showed the existence of three different clusters. And these three different clusters might be associated with the genes involved in three plant types. The results also suggested that there were plentiful SSR polymorphisms among peanut germplasm accessions in RDA (Rural Development Administration, Korea) Genebank and SSRs might play an important role in evaluating peanut accessions and cultivar improvement.

First Report of Soft Rot Induced by Dickeya dadantii on Euphorbia hypogaea in Korea

  • Ismaila Yakubu;Ji Ho Song;Yun Ju Lee;Min A Son;Su Hyeon Han;Hyun Gi Kong
    • Research in Plant Disease
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    • v.30 no.1
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    • pp.95-98
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    • 2024
  • In a survey conducted in March 2023, Euphorbia hypogaea plants cultivated within greenhouses in Yongin, Korea exhibited water-soaked areas near the stem base, close to the soil. Subsequent isolation from diseased E. hypogaea led to the identification of a bacterial strain, designated as CBNUMPBL-103. The isolate was identified as Dickeya dadantii through sequencing of the 16s rRNA and phylogenetic analysis. The pathogenicity of the isolate was confirmed by inoculating it into healthy E. hypogaea, resulting in the manifestation of similar symptoms observed during the survey. The re-isolated strain recovered from inoculated plants showed a similar morphology with the inoculated strain. This is the first documentation of D. dadantii causing soft rot of E. hypogaea in Korea.

Correlative Effect of Adenine Sulphate and Benzylaminopurine on the Regeneration Potentialily in Cotyledonary Explants of Groundnut (Arachis hypogaea L.)

  • Palanivel, S.;Jayabalan, N.
    • Journal of Plant Biotechnology
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    • v.2 no.1
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    • pp.21-24
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    • 2000
  • An efficient method of shoot regeneration of peanut is described. In vitro shoot organogenesis from the callus of cotyledon explants of Arachis hypogaea L. was stimulated by addition of Adenine sulphate (Ads) along with 6 - benzylaminopurine (BAP) and - napthalene acetic acid (NAA). Ads (13 ${\mu}{\textrm}{m}$) had a stimulatory effect on shoot bud differentiation when combined with BAP (13 ${\mu}{\textrm}{m}$) and NAA (2 ${\mu}{\textrm}{m}$). Shoot organogenesis was markedly higher (92%) from callus induced on Ads, BAP and NAA combined media than from those formed by the individual supplementation of Ads or BAP with NAA. The shoots elongated on the media with GA$_3$ (1 ${\mu}{\textrm}{m}$). Elongated plantlets rooted with MS media containing IBA (9 ${\mu}{\textrm}{m}$).

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Identification of 3-methoxy-4-hydroxybenzoic acid and 4-hydroxybenzoic acid with Antioxidative and Antimicrobial Activity from arachis hypogaea Shell (땅콩껍질에서 항균 및 항산화활성이 있는 3-methoxy-4-hydroxybenzoic acid와 4-hydroxybenzoic acid의 동정)

  • 위지향;박근형
    • KSBB Journal
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    • v.15 no.5
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    • pp.464-468
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    • 2000
  • The methanol extract of Arachis hypogaea shell showed antioxidative and antimicrobial activity. The methanol extract was successively purified by solvent fractionation, silica gel adsorption column chromatography, Sephadex LH-20 column chromatography and octadecylsilane column chromatography. The purified active substances were isolated by high performance liquid chromatography, and were identified as 3-methoxy-4-hydroxybenzoic acid and 4-hydroxybenzoic acid by LC-MS and GC-MS. The amount of 3-methoxy-4-hydroxybenzoic acid and 4-hydroxybenzoic acid were 3.8mg and 9.8 mg per kg of shell, respectively.

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Seed Development and Germinability of Unripened Seed in Peanuts (땅콩 종실의 발육과 미숙종자의 발아력)

  • 김석동;이성우;박장환
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.42 no.3
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    • pp.333-337
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    • 1997
  • Pods and kernels of two peanut(Arachis hypogaea L.) cultivars, a Shinpung type, Shindaekwangtangkong and a Virginia bunch type, Namdaetangkong were harvested periodically after the gynophore earthing in the field condition and their developments were monitored by observation of the changes in size, weight and moisture content. Seed germinability and seedling vigor were tested to set up a method for hastening the generation advancement in breeding procedure. Pods and kernels reached the maximum in size at 30 to 40 days after gynophore earthing and at that time the kernels had one hundred percent germinability and the seedlings sprouted from those kernels exhibited practically acceptable viability enough to grow for the next generation.

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α-Glucosidase Inhibitory Activity of the Ethanol Extract of Peanut (Arachis hypogaea L.) Skin (땅콩 속껍질 에탄올 추출물의 알파-글루코시데이즈 억제활성)

  • Ha, Tae Joung;Lee, Myoung Hee;Oh, Eunyoung;Kim, Jung In;Song, Seok Bo;Kwak, Doyeon
    • Korean Journal of Medicinal Crop Science
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    • v.28 no.1
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    • pp.21-28
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    • 2020
  • Background: Owing to its high efficiency in lipid and protein production, peanut (Arachis hypogaea L.) is considered one of most important crops world-wide. The kernels of peanuts are undoubtedly the most important product this plant, whereas the skin is almost completely neglected in nutraceutical terms. However, peanut skin contains potentially health-promoting phenolics and dietary fiber, and there is considerable potential for commercial exploitation. In this study, we evaluated the α-glucosidase inhibitory activity of an extract of peanut skin (PS). Methods and Results: The α-glucosidase inhibitory effects of 80% ethanol extracts of peanut (A. hypogaea L. 'Sinpalkwang') skin were evaluated and found to have a half-maximal inhibitory concentration (IC50) value of 1.2 ㎍/㎖. Progress curves for enzyme reactions were recorded spectrophotometrically, and the inhibition kinetics revealed time-dependent inhibition with enzyme isomerization. Furthermore, using ultra-high performance liquid chromatography combined with quadrupole-orbitrap mass spectrometry, we identified 26 compounds in the peanut skin extract, namely, catechin, epicatechin, and 24 proanthocyanidins. Conclusions: The results suggest that peanut skin can be utilized as an effective source of α-glucosidase inhibition in functional foods and nutraceuticals.

The Development and Selection of SSR Markers for Identification of Peanut (Arachis hypogaea L.) Varieties in Korea

  • Han, Sang-Ik;Bae, Suk-Bok;Ha, Tae Joung;Lee, Myong-Hee;Jang, Ki-Chang;Seo, Woo-Duck;Park, Geum-Yong;Kang, Hang-Won
    • Korean Journal of Breeding Science
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    • v.43 no.2
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    • pp.133-138
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    • 2011
  • The groundnut or cultivated peanut (Arachis hypogaea L.) in Korea consists of 36 domestic varieties which have been developed and registered as cultivars for the public during last 25 years. To screen and identify of Korean peanut varieties and genetic resources, we present a simple and reliable method. A methodology based on simple sequence repeat (SSR) markers developed and widely used for prominent gene identification and variety discrimination. For identification of those 36 Korean peanut varieties, 238 unique peanut SSR markers were selected from some previously reported results, synthesized and used for polymerase chain reaction (PCR). Data were taken through acryl amide gel electrophoresis and changed into proper formats for application of data mining analysis using Biomine (all-in-one functional genomics data mining program). Consequently, twelve SSR primers were investigated and revealed the differences between those 36 varieties. These primer pairs amplified 27 alleles with an average of 2.3 allele per primer pair. In addition, those results showed genetic relationship by classification method within 36 varieties. The approach described here could be applied to monitoring of our varieties and adapting to peanut breeding program.