Journal of Plant Biotechnology

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Correlative Effect of Adenine Sulphate and Benzylaminopurine on the Regeneration Potentialily in Cotyledonary Explants of Groundnut (Arachis hypogaea L.)

  • Palanivel, S. (Department of Microbilogy, J.J College of Arts and Science, India) ;
  • Jayabalan, N. (Plant Tissue Culture Unit, Department of Plant Science Bharathidasant University, India)
  • Published : 2000.01.01
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Abstract

An efficient method of shoot regeneration of peanut is described. In vitro shoot organogenesis from the callus of cotyledon explants of Arachis hypogaea L. was stimulated by addition of Adenine sulphate (Ads) along with 6 - benzylaminopurine (BAP) and - napthalene acetic acid (NAA). Ads (13 ${\mu}{\textrm}{m}$) had a stimulatory effect on shoot bud differentiation when combined with BAP (13 ${\mu}{\textrm}{m}$) and NAA (2 ${\mu}{\textrm}{m}$). Shoot organogenesis was markedly higher (92%) from callus induced on Ads, BAP and NAA combined media than from those formed by the individual supplementation of Ads or BAP with NAA. The shoots elongated on the media with GA$_3$ (1 ${\mu}{\textrm}{m}$). Elongated plantlets rooted with MS media containing IBA (9 ${\mu}{\textrm}{m}$).

Keywords

References

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