한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제28권1호
  • /
  • Pages.21-28
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  • 2020
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  • 1225-9306(pISSN)
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  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
권호 표지
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땅콩 속껍질 에탄올 추출물의 알파-글루코시데이즈 억제활성

α-Glucosidase Inhibitory Activity of the Ethanol Extract of Peanut (Arachis hypogaea L.) Skin

  • 하태정 (농촌진흥청 국립식량과학원 밭작물개발과) ;
  • 이명희 (농촌진흥청 국립식량과학원 밭작물개발과) ;
  • 오은영 (농촌진흥청 국립식량과학원 밭작물개발과) ;
  • 김정인 (농촌진흥청 국립식량과학원 밭작물개발과) ;
  • 송석보 (농촌진흥청 국립식량과학원 밭작물개발과) ;
  • 곽도연 (농촌진흥청 국립식량과학원 밭작물개발과)
  • 투고 : 2019.10.31
  • 심사 : 2019.12.16
  • 발행 : 2020.02.28
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초록

Background: Owing to its high efficiency in lipid and protein production, peanut (Arachis hypogaea L.) is considered one of most important crops world-wide. The kernels of peanuts are undoubtedly the most important product this plant, whereas the skin is almost completely neglected in nutraceutical terms. However, peanut skin contains potentially health-promoting phenolics and dietary fiber, and there is considerable potential for commercial exploitation. In this study, we evaluated the α-glucosidase inhibitory activity of an extract of peanut skin (PS). Methods and Results: The α-glucosidase inhibitory effects of 80% ethanol extracts of peanut (A. hypogaea L. 'Sinpalkwang') skin were evaluated and found to have a half-maximal inhibitory concentration (IC50) value of 1.2 ㎍/㎖. Progress curves for enzyme reactions were recorded spectrophotometrically, and the inhibition kinetics revealed time-dependent inhibition with enzyme isomerization. Furthermore, using ultra-high performance liquid chromatography combined with quadrupole-orbitrap mass spectrometry, we identified 26 compounds in the peanut skin extract, namely, catechin, epicatechin, and 24 proanthocyanidins. Conclusions: The results suggest that peanut skin can be utilized as an effective source of α-glucosidase inhibition in functional foods and nutraceuticals.

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참고문헌

  1. Case A and Stein RL. (2007). Kinetic analysis of the interaction of tissue transglutaminase with a nonpeptidic slow-binding inhibitor. Biochemistry. 46:1106-1115. https://doi.org/10.1021/bi061787u
  2. Caspary WF. (1978). Sucrose malabsorption in man after ingestion of $\alpha$-glucosidehydrolase inhibitor. The Lancet. 311: 1231-1233. https://doi.org/10.1016/S0140-6736(78)92466-2
  3. Chen L, Yan F, Chen W, Zhao L, Zhang J, Lu Q and Liu R. (2018). Procyanidin from peanut skin induces antiproliferative effect in human prostate carcinoma cell DU145. Chemico-Biological Interactions. 288:12-23. https://doi.org/10.1016/j.cbi.2018.04.008
  4. Copeland RA. (2000). Enzyme: A practical introduction to structure, mechanism, and data analysis. Wiley-VCH. New York. NY, USA. p.266-304.
  5. Dong XQ, Zou B, Zhang Y, Ge ZZ, Du J and Li CM. (2013). Preparation of A-type proanthocyanidin dimers from peanut skins and persimmon pulp and comparison of the antioxidant activity of A-type and B-type dimers. Fitoterapia. 91:128-139. https://doi.org/10.1016/j.fitote.2013.08.019
  6. Feldman EB. (1999). Assorted monounsaturated fatty acids promote healthy hearts. American Journal of Clinical Nutrition. 70:953-954. https://doi.org/10.1093/ajcn/70.6.953
  7. Francisco MLDL and Resurreccion AVA. (2008). Functional components in peanuts. Critical Reviews in Food Science and Nutrition. 48:715-746. https://doi.org/10.1080/10408390701640718 (cited by 2019 Oct 12).
  8. Guo S, Ai M, Liu J, Luo Z, Yu J, Li Z and Jiang A. (2019). Physicochemical, conformational properties and ACE-inhibitory activity of peanut protein marinated by aged vinegar. LWT-Food Science and Technology. 101:469-475. https://doi.org/10.1016/j.lwt.2018.11.058
  9. Ha TJ and Kubo I. (2007). Slow-binding inhibition of soybean lipoxygenas-1 by dodecyl gallate. Journal of Agricultural and Food Chemistry. 55:446-451. https://doi.org/10.1021/jf0616457
  10. Ha TJ, Lee JH, Lee MH, Lee BW, Kwon HS, Park CH, Shin KB, Kim HT, Baek IY and Jang DS. (2012). Isolation and identification of phenolic compounds from the seeds of Perilla frutescens(L.) and their inhibitory activities against $\alpha$-glucosidase and aldose reductase. Food Chemistry. 135:1397-1403. https://doi.org/10.1016/j.foodchem.2012.05.104
  11. Ha TJ, Park JE, Kang BK, Kim HS, Shin SO, Seo JH, Oh EY, Kim SU and Kwak DY. (2019). $\alpha$-Glucosidase inhibitory activity of isoflavones and saponins from soybean(Glycine max L.) and comparisons of their constituents during heat treatments. Journal of Korean Society of Food Science and Nutrition. 48:953-960.
  12. Ha TJ, Song SB, Ko JY, Park CH, Ko JM, Choe ME, Kwak DY and Lee JH. (2018). Isolation and identification of $\alpha$-glucosidase inhibitory constituents from the seed of Vigna nakashimae: Enzyme kinetic study with active phytochemical. Food Chemistry. 266:483-489. https://doi.org/10.1016/j.foodchem.2018.06.039
  13. Huang CP, Au LC, Chiou RYY, Chung PC, Chen SY, Tang WC, Chang CL, Fang WH and Lin SB. (2010). Arachidin-1, a peanut stilbenoid, induces programmed cell death in human leukemia HL-60 cells. Journal of Agricultural and Food Chemistry. 58:12123-12129. https://doi.org/10.1021/jf102993j
  14. Kim MY, Kim HJ, Kim MH, Lee JY, Lee YY, Lee BK and Lee BW. (2019). Changes in the physiological activities of peanut and defatted peanut extracts according to cultivars. Journal of the Korean Society of Food Science and Nutrition. 48:847-855.
  15. Kim TW, Choi HJ, Eom SH, Lee JM and Kim TH. (2014). Potential $\alpha$-glucosidase inhibitors from thermal transformation of (+)-catechin. Bioorganic & Medicinal Chemistry Letters. 24:1621-1624. https://doi.org/10.1016/j.bmcl.2014.01.027
  16. Knauft DA and Ozias-Akins P. (1995). Recent methodologies for germplasm enhancement and breeding. In Pattee HE and Stalker HT. (ed.). Advances in peanut science. American Peanut Research and Education Society. Still water. OK, USA. p.54-94.
  17. Lebovitz HE. (1997). Alpha-glucosidase inhibitors. Endocrinology and Metabolism Clinics of North America. 26:539-551. https://doi.org/10.1016/s0889-8529(05)70266-8
  18. Limmongkon A, Janhom P, Amthong A, Kawpanuk M, Nopprang P, Poohadsuan J, Somboon T, Saijeen S, Surangkul D, Srikummool M and Boonsong T. (2017). Antioxidant activity, total phenolic, and resveratrol content in five cultivars of peanut sprouts. Asian Pacific Journal of Tropical Biomedicine. 7:332-338. https://doi.org/10.1016/j.apjtb.2017.01.002
  19. Ostlund RE, Racette SB and Stenson WF. (2002). Effects of trace components of dietary fat on cholesterol metabolism: Phytosterols, oxysterols, and squalene. Nutrition Reviews. 60:349-359. https://doi.org/10.1301/00296640260385793
  20. Pae SB, Hwang CD, Kim SU, Lee MH, Shim KB, Park CH, Lee CK, Baek LY and Lee JK. (2016). A new large grain and high-yielding virginia type peanut cultivar 'Sinpalkwang'. Korean Journal of Breeding Science. 48:66-71. https://doi.org/10.9787/KJBS.2016.48.1.066
  21. Park JH, Baek MR, Lee BH, Yon GH, Ryu SY, Kim YS, Park SU and Hong KS. (2009). $\alpha$-Glucosidase and $\alpha$-amylase inhibitory activity of compounds from roots extract of Pueraria thunbergiana. Korean Journal of Medicinal Crop Science. 17:357-362.
  22. Ricciarelli R, Zingg JM and Azzi A. (2001). Vitamin E: Protective role of a Janus molecule. The FASEB Journal. 15:2314-2325. https://doi.org/10.1096/fj.01-0258rev
  23. Saavedra-Delgado AM. (1989). The many faces of the peanut. Allergy and Athma Proceedings. 10:291-294.
  24. Sabate J, Oda K and Ros E. (2010). Nut consumption and blood lipid levels: A pooled analysis of 25 intervention trials. Archives of Internal Medicine. 170:821-827. https://doi.org/10.1001/archinternmed.2010.79
  25. Simithy J, Fuanta NR, Alturki M, Hobrath JV, Wahba AE, Pina I, Rath J, Hamann MT, DeRuiter J, Goodwin DC and Calderon AI. (2018). Slow-binding inhibition of Mycobacterium tuberculosis shikimate kinase by manzamine alkaloids. Biochemistry. 57:4923-4933. https://doi.org/10.1021/acs.biochem.8b00231
  26. Tatsuno T, Jinno M, Arima Y, Kawabata T, Hasegawa T, Yahagi N, Takano F and Ohta T. (2012). Anti-inflammatory and anti-melanogenic proanthocyanidin oligomers from peanut skin. Biological and Pharmaceutical Bulletin. 35:909-916. https://doi.org/10.1248/bpb.35.909
  27. Van De Laar FA, Lucassen PL, Akkermans RP, Van De Lisdonk EH, Rutten GE and Van Weel C. (2005). $\alpha$-Glucosidase inhibitors for patients with type 2 diadetes. Diabetes Care. 28:154-162. https://doi.org/10.2337/diacare.28.1.154
  28. Wild S, Roglic G, Green A, Sicree R and King H. (2004). Global prevalence of diabetes: Estimates for the year 2000 and projections for 2030. Diabetes Care. 27:1047-1053. https://doi.org/10.2337/diacare.27.5.1047
  29. Woodroof JP. (1983). Peanuts: Production, processing, products. AVI Publishing Company. Westport. CT, USA. p.1-50.
  30. Yuk HJ, Curtis-Long MJ, Ryu HW, Jang KC, Seo WD, Kim JY, Kang KY and Park KH. (2011). Pterocarpan profiles for soybean leaves at different growth stages and investigation of their glycosidase inhibitions. Journal of Agricultural and Food Chemistry. 59:12683-12690. https://doi.org/10.1021/jf203326c