• Journal of Physiology & Pathology in Korean Medicine
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• v.23 no.6
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• pp.1379-1384
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• 2009

The purpose of this study is to investigate the effect of water extract from Artemisiae Argi Folium Fermented with Lactobacillus pentosus (AFL) on hydrogen peroxide production within human hepatocyte HepG2 cells treated with gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde. AFL (0~400 ug/mL) was treated with gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde. And the intracellular productions of hydrogen peroxide were measured by dihydrorhodamine 123 (DHR) assay. AFL showed the restoration of the intracellular productions of hydrogen peroxide which were reduced by gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde in HepG2 Cells. AFL could be supposed to have the hepatoprotective effect related with hepatocytologic signaling activity against gallic acid, EtOH, nicotine, acetaminophen, and acetaldehyde.

• New & Renewable Energy
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• v.5 no.4
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• pp.80-85
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• 2009

The effects of various organic wastes on anaerobic fermentative hydrogen production were studied using enriched mixed microflora in batch tests. Rotten fruit, corn powder and organic wastewater enriched with sulfate (up to 1,000 mg/L) were used for experiments. Maximum hydrogen production (547.1 mL) was observed from rotten apple with initial substrate concentration of 132.2 g COD/L. The experimental result on sulfate enriched organic wastewater indicated that hydrogen production is not adversely influenced by relatively high sulfate concentration. Residual sulfate content remained at 96-98 % after 75 hours of reaction, which showed that no major sulfate reduction was occurred at pH 5.3-5.5 in the reactor. The volatile fatty acid (VFA) fractions produced during the reaction was in the order of butyrate > acetate > propionate in all experiments. The results of this study would be useful for controlling the conditions on fermentative hydrogen production using different feedstocks.

• KSBB Journal
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• v.20 no.6
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• pp.464-469
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• 2005

For the biological production of hydrogen, a new fermentative hydrogen-producing bacterium, Enterobacter sp. SNU-1453, was isolated from a domestic landfill. During the culture of this bacterium, pH significantly decreased with the accumulation of various organic acids, and consequently this inhibited the production of hydrogen. It was found that the metabolic flux in this bacterium depended on the pH and affected the hydrogen production. A butanediol pathway was dominant during the fermentation when pH was not controlled. By controlling the pH at 7 this pathway can be shifted to a mixed acid pathway, which is favorable to the production of hydrogen.

• Journal of Korean Society of Environmental Engineers
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• v.30 no.12
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• pp.1239-1244
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• 2008

In this study, PCR-DGGE was conducted to investigate the variations of microbial community according to pH conditions from pH 3 to pH 10 during anaerobic fermentation process of hydrogen production. Maximum hydrogen yield was 1.8 mol $H_2$/mol substrate at pH 5. The microbial growth rate was not proportional to the hydrogen production rate at each pH. Variations of microbial community was observed at each condition from PCR-DGGE experiment of 16s rDNA. Klebsiella was main species of the microbial community. Streptococcus and Clostridium were mainly contributed for hydrogen production.

• Asian-Australasian Journal of Animal Sciences
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• v.23 no.6
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• pp.700-707
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• 2010

Effects of protease-resistant antimicrobial substances (PRA) produced by Lactobacillus plantarum and Leuconostoc citreum on rumen methanogenesis were examined using the in vitro continuous methane quantification system. Four different strains of lactic acid bacteria, i) Lactococcus lactis ATCC19435 (Control, non-antibacterial substances), ii) Lactococcus lactis NCIMB702054 (Nisin-Z), iii) Lactobacillus plantarum TUA1490L (PRA-1), and iv) Leuconostoc citreum JCM9698 (PRA-2) were individually cultured in GYEKP medium. An 80 ml aliquot of each supernatant was inoculated into phosphate-buffered rumen fluid. PRA-1 remarkably decreased cumulative methane production, though propionate, butyrate and ammonia N decreased. For PRA-2, there were no effects on $CH_4$ and $CO_2$ production and fermentation characteristics in mixed rumen cultures. The results suggested that PRA-1 reduced the number of methanogens or inhibited utilization of hydrogen in rumen fermentation.

• Journal of the Korea Organic Resources Recycling Association
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• v.24 no.4
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• pp.5-9
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• 2016

In the modern industrial society, huge amount of organic wastes have exceeded the society's self-cleaning capability, caused pollution of the whole environment, including water quality, soil, and the air, and become a big burden of waste treatment. Moreover, the emission of green house gases brought by the continual combustion of fossil fuels has facilitated the global warming. The simultaneous effect of initial and operational pH on $H_2$ yield was expressed using mathematical equation and optimized. The optimal initial and cultivation pH was 7.50 and 6.01, respectively. Addition of livestock wastewater to food waste substantially decreased the amount of alkali requirement and also improved the $H_2$ fermentation performance.

• Transactions of the Korean hydrogen and new energy society
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• v.29 no.6
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• pp.648-653
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• 2018

Bran of barley causes high viscosity in bioethanol production due to the large amount of ${\beta}$-glucans and fiber. High viscosity is the main cause of decreased productivity and decreased facility efficiency in ethanol production. In order to prevent high viscosity, this study investigated the possibility of bioethanol from barley by debranning. As a result, it was able to reduced the viscosity (22.8 cP to 17.5 cP). And the fermentation speed and yield were improved as the activity of the enzyme and activity of yeast was also increased was improved due to the removal of non-fermentable components. In conclusion, debranning was advantageous in two ways. Firstly, bran removal increased the starch content of the feedstock and decreased viscosity of mash, improving ethanol fermentation. Secondly, by-products produced by debranning can use valuable products. It was remarkable results to the feasibility of bioethanol production from debranned barley.

• Proceedings of the Korean Environmental Health Society Conference
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• 2005.06a
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• pp.394-398
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• 2005

This study aimed to develop a novel anaerobic two-stage process converting food waste to $H_2$ and $CH_2$. The anaerobic two-stage process was devised by combining hydrogen fermentation with methane fermentation. At the high loading rate of 12.3 kg $Vs/m^3/d$, it could remove 72.5%of VS and convert $VS_{removed}$ to $H_2$ (28.2%) and $CH_4$ (69.9%) on COD basis in 8 days.

• YAKHAK HOEJI
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• v.47 no.2
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• pp.93-97
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• 2003

Kombucha fermentation broth has been used as a popular health beverage and an alternative therapy with prophylactic and therapeutic benefit. We tried to establish optimal culture conditions for Kombucha fermentation in milk and to investigate cytotoxicity and antioxidative enzyme activity of Kombucha broth against normal human fibroblasts. The optimal conditions of Kombuch culture were established to 3$0^{\circ}C$, 20∼23 hours by DPPH radical scavenging test. There were positive effects on cell growth while no cytotoxicity against primary normal human diploid fibroblasts was found. The activites of glutathione peroxide and catalase in the cells treated by hydrogen peroxide (1 mM) alone and by hydrogen peroxide with Kombucha broth (1 mg/mι) were significantly different (p＜0.05). These results suggest that Kombucha broth could be developed as an antioxidant agent for a new cosmetic material.

• Journal of Environmental Health Sciences
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• v.26 no.2
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• pp.1-5
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• 2000

연속형 혐기성처리 반응조에서 배양된 수소발생 슬러지를 이용하여 증온 조건에서 회분식 혐기성 처리방법으로 유기성 폐수로부터 전환되는 수소가스 및 대사산물들에 대한 연구를 수행하였다. 수소발생에 대한 기질로는 sucrose를 이용하였다. 처리과정에서 발생된 누적수소가스, 휘발성지방산(VFAs) 및 solvents는 Gompertz equation을 이용한 비선형회귀분석을 통하여 계산하였다. 처리과정 중 수소가스는 반응초기에 발생하였고, 발생된 가스내 수소가스가 차지하는 비율은 약 20%이었다. 반응 전과정에서 메탄가스는 발생하지 않았다. 비수소가스발생율은 sucrose 농도가 40 g/l일 때 0.956 ml/g VSs/h이었으며, sucrose 농도가 300g/l의 경우는 0.011 ml/g VSS/h이었다. 수소가 발생하는 기간 동안 VFAs의 생성은 acetate, butyrate의 순으로 높게 생성되었으나, propionate로의 전환은 발견되지 않았다. solvents의 경우 butanol이 가장 높게 발생하였다.