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pH-dependent Metabolic Flux Shift in Novel Hydrogen-Producing Bacterium Enterobacter sp. SNU-1453  

Shin, Jong-Hwan (School of Chemical and Biological Engineering, Seoul National University)
Yoon, Jong-Hyun (School of Chemical and Biological Engineering, Seoul National University)
Ahn, Eun-Kyoung (School of Chemical and Biological Engineering, Seoul National University)
Sim, Sang-Jun (Department of Chemical Engineering, Sungkyunkwan University)
Kim, Mi-Sun (Korea Institute of Energy Research)
Park, Tai-Hyun (School of Chemical and Biological Engineering, Seoul National University)
Publication Information
KSBB Journal / v.20, no.6, 2005 , pp. 464-469 More about this Journal
Abstract
For the biological production of hydrogen, a new fermentative hydrogen-producing bacterium, Enterobacter sp. SNU-1453, was isolated from a domestic landfill. During the culture of this bacterium, pH significantly decreased with the accumulation of various organic acids, and consequently this inhibited the production of hydrogen. It was found that the metabolic flux in this bacterium depended on the pH and affected the hydrogen production. A butanediol pathway was dominant during the fermentation when pH was not controlled. By controlling the pH at 7 this pathway can be shifted to a mixed acid pathway, which is favorable to the production of hydrogen.
Keywords
Enterobacter sp. SNU-1453; pH; metabolic flux shift;
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