Browse > Article

Change of Microbial Communities in Fermentative Hydrogen Production at Difference Cultivation pHs  

Jun, Yoon-Sun (Department of Environmental Engineering, Seoul National University of Technology)
Lee, Kwan-Yong (Department of Environmental Engineering, Seoul National University of Technology)
Cho, Yoon-A (Department of Environmental Engineering, Seoul National University of Technology)
Lee, Tae-Jin (Department of Environmental Engineering, Seoul National University of Technology)
Publication Information
Journal of Korean Society of Environmental Engineers / v.30, no.12, 2008 , pp. 1239-1244 More about this Journal
Abstract
In this study, PCR-DGGE was conducted to investigate the variations of microbial community according to pH conditions from pH 3 to pH 10 during anaerobic fermentation process of hydrogen production. Maximum hydrogen yield was 1.8 mol $H_2$/mol substrate at pH 5. The microbial growth rate was not proportional to the hydrogen production rate at each pH. Variations of microbial community was observed at each condition from PCR-DGGE experiment of 16s rDNA. Klebsiella was main species of the microbial community. Streptococcus and Clostridium were mainly contributed for hydrogen production.
Keywords
Anaerobic Fermentation; Hydrogen Production; Klebsiella; Clostridium; Streptococcus; PCR-DGGE;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Levin, D. B., Pitt, L., and Love, M., "Biohydrogen production: prospects and limitations to practical application," Int. J. Hydrogen Energy, 29, 173-185(2004)   DOI   ScienceOn
2 Chen, X., Sun, Y., Xiu, Z., Li, X., Zhang, D., "Stoichiometric analysis of biological hydrogen production by fermentative bacteria," Int. J. Hydrogen Energy, 31, 539-549(2006)   DOI   ScienceOn
3 Ueno, Y., Haruta, S., Ishii, M., and Igarashi, Y., "Characterization of a microorganism isolated from the effluent of hydrogen fermentation by microflora," J. Bioscience and Bioengineering, 92(4), 397-400(2001)   DOI
4 Vreas, L., Forney, L., Daae, F. L., and Torsvik, V., "Distribution of bacterioplankton in meromictic lake selenvannet, as determined by denaturing gradient gel electrophoresis of PCR-Amplified gene fragments coding for 16S rRNA," Appl. Environ. Microbiol., 63(9), 3367-3373(1997)   PUBMED
5 Mannix Salvador Pedro, Shin Haruta, Masaru Hazaka, Rumiko Shimada, Chie Yoshida, Koichiro Hiura, Masaharu Ishii, and Yasuo Igarashi, "Denaturing gradient gel electrophoresis analyses of microbial community from fieldscale composter", J. Bioscience and Bioengineering, 91(2), 159-165(2001)   DOI
6 Chen, C.-C. and Lin, C.-Y., "Using sucrose as a substrate in an anaerobic hydrogen-producing reactor," Adv. Environ. Res., 7, 695-699(2003)   DOI   ScienceOn
7 Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A., and Smith, F., "Colorimetric method for determination of sugars and related substances," Anal. Chem., 28(3), 350-356(1956)   DOI
8 Minnan, L., Jinli, H., Xuijuan, W., Jinzao, C., Chuannan, L., Fengzhang, Z., Liangshu, X., "Isolation and characterization of a high H2-Producing strain Klebsiella oxytoca HP1 from a hot spring," Res. Microbiol., 156, 76-81 (2005)   DOI   ScienceOn
9 Nguyen, T. A. D., Kim, Y. P., Kim, M. S., Oh, Y. K., Sim, S. J., "Optimization of hydrogen production by hyperthermophilic eubacteria, Thermotoga maritima and Thermotoga neapolitana in batch fermentation," Int. J. Hydrogen Energy (accepted), (2007)
10 Felgenstein, J., "Confidence limits on phylogenetics: an approach using the bootstrap," Evolution, 39, 783-791 (1985)   DOI   ScienceOn
11 Okamoto, M., Miyahara, T., Mizuno, O., and Noike, T., "Biological hydrogen potential of materials characteristic of the organic fraction of municipal solid wastes," Water Sci. Technol., 41(3), 25-32(2000)   PUBMED
12 Heyndrix, M., De Vos, P., Thibau, B., Stevens, P., and JI De Ley, "Effect of various external factorson the fermentative production of hydrogen gas from glucoes by Clostridium butyricum strains in batch culture system," Appl. Microbiol, 9, 163-168(1987)   DOI
13 Saitou, N., Nei, M., "The neighbour-joining method: a new method for constructing phylogenetic trees," Mol. Biol. Evol., 4, 406-425(1987)   PUBMED
14 Zaborsky, O. R., "Biohydrogen," Plenum Press, Newyork, pp. 10-18(1998)
15 Auch, A. F., Henz, S. R., Holland, B. R., and Goker, M., "Genome BLAST distance phylogenies inferred from whole plastid and whole mitochondrion genome sequences," BioMed Central Ltd.(2006)
16 Fang, H. H. P., and H. Liu, "Effect of pH on hydrogen production from glucose by a mixed culture," Bioresour. Technol., 82, 87-93(2002)   DOI   ScienceOn
17 Logan, B. E., OH, S. E., Kim, I. S., Ginkel, S. V., "Biological hydrogen production measured in batch anaerobic respirometers," Environ. Sci. Technol., 36, 2530-2535(2002)   DOI   ScienceOn
18 Prescott, Harley, Klein, "Microbiology," Mc Graw Hill Higher Education, pp. 412-413(2003)
19 Van Andel, J. G., Zoutberg, G. R., Crabbendam, P. M., and Breau, A. M., "Glucose fermentation by Clostridium butyricum grown under a self generated gas atmosphere in chemostat culture," Appl. Microbiol. Biotechnol., 23, 21-26(1985)   DOI
20 Hung, C. H., Cheng, C. H., Cheng, L. H., Liang, C. M., Lin, C. Y., "Application of Clrostridium-specific PCR primers on the analysis of dark fermentation hydrogenproducing bacterial community," Int. J. Hydrogen Energy (2007)
21 Kimura, M., "A simple method for estimating evolutionary rate of base substitutions through comparative studies of nucleotide sequences," J. Mol. Evol., 16, 111-120 (1980)   DOI
22 Hollibaugh, J. T., Bano, N., and Ducklow, H., "Widespread distribution in polar oceans of a 16S rRNA gene sequence with affinity to Nitrosospira-like ammonia-oxidizing bacteria," Appl. Environ. Microbiol., 68(3), 1478-1484(2002)   DOI