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Antioxidant Effect of Kombucha Broth Against Scenescence Induced Normal Human Diploid Fibroblasts with Oxygen Free Radicals  

이상은 (STC 생명과학연구원 신약개발연구소)
최진석 (한국화학시험연구원)
이강훈 (STC 생명과학연구원 신약개발연구소)
김국환 (동덕여자대학교 약학대학)
권영이 (STC 생명과학연구원 신약개발연구소)
Publication Information
YAKHAK HOEJI / v.47, no.2, 2003 , pp. 93-97 More about this Journal
Abstract
Kombucha fermentation broth has been used as a popular health beverage and an alternative therapy with prophylactic and therapeutic benefit. We tried to establish optimal culture conditions for Kombucha fermentation in milk and to investigate cytotoxicity and antioxidative enzyme activity of Kombucha broth against normal human fibroblasts. The optimal conditions of Kombuch culture were established to 3$0^{\circ}C$, 20∼23 hours by DPPH radical scavenging test. There were positive effects on cell growth while no cytotoxicity against primary normal human diploid fibroblasts was found. The activites of glutathione peroxide and catalase in the cells treated by hydrogen peroxide (1 mM) alone and by hydrogen peroxide with Kombucha broth (1 mg/mι) were significantly different (p＜0.05). These results suggest that Kombucha broth could be developed as an antioxidant agent for a new cosmetic material.
Keywords
Antioxidant activity; kombucha broth; human diploid fibroblasts;
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